##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512731_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96181 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.414530936463542 33.0 31.0 34.0 28.0 34.0 2 31.45558894168287 33.0 31.0 34.0 28.0 34.0 3 31.474043730050635 33.0 31.0 34.0 28.0 34.0 4 35.030723323733376 37.0 35.0 37.0 32.0 37.0 5 34.81505702789532 37.0 35.0 37.0 32.0 37.0 6 34.699223339328974 37.0 35.0 37.0 32.0 37.0 7 34.68570715629906 37.0 35.0 37.0 32.0 37.0 8 34.689366922781005 37.0 35.0 37.0 32.0 37.0 9 36.15929341553945 39.0 37.0 39.0 32.0 39.0 10 36.0216466869756 39.0 37.0 39.0 31.0 39.0 11 36.16126885767459 39.0 37.0 39.0 32.0 39.0 12 36.05923207286262 39.0 37.0 39.0 31.0 39.0 13 36.04480094821223 39.0 37.0 39.0 31.0 39.0 14 36.9891454653206 40.0 37.0 41.0 31.0 41.0 15 36.87273993824144 40.0 37.0 41.0 31.0 41.0 16 36.740302138676036 40.0 37.0 41.0 30.0 41.0 17 36.96534658612408 40.0 37.0 41.0 31.0 41.0 18 36.93803349933979 40.0 37.0 41.0 31.0 41.0 19 36.952755741778525 40.0 37.0 41.0 31.0 41.0 20 37.043147815057026 40.0 37.0 41.0 31.0 41.0 21 36.997348748713364 40.0 37.0 41.0 31.0 41.0 22 36.85947328474439 40.0 37.0 41.0 31.0 41.0 23 36.7437539638806 40.0 37.0 41.0 31.0 41.0 24 36.60728210353396 40.0 36.0 41.0 30.0 41.0 25 36.48207026335763 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37947131914699384 1101 2 0.4464580195666876 1101 3 0.548106865283426 1101 4 0.2907482370884651 1101 5 -0.10861670472959872 1101 6 0.10468497812048128 1101 7 0.022243720500242148 1101 8 0.4131880304955544 1101 9 0.1819921357155323 1101 10 0.5713321864904444 1101 11 0.14009813092032886 1101 12 0.18167370702787622 1101 13 -0.06719349354554538 1101 14 -0.7080207758883432 1101 15 -0.15068427150154662 1101 16 -0.05507199812625885 1101 17 0.31532184232444394 1101 18 0.8473598239283788 1101 19 0.4500138715342601 1101 20 0.4936526798531986 1101 21 1.2919261947017233 1101 22 1.3614758469453605 1101 23 0.5771048907995819 1101 24 0.6245617387499749 1101 25 0.3247767040892384 1102 1 0.7793079204541868 1102 2 1.1041377581287755 1102 3 0.9353617672442063 1102 4 0.6141142501603625 1102 5 0.9510238181462185 1102 6 1.1131817101466268 1102 7 1.2541064655982836 1102 8 0.7973383572929365 1102 9 1.4038875605521355 1102 10 1.4265609446603804 1102 11 1.575719045953008 1102 12 0.9713795893808168 1102 13 1.428067944362951 1102 14 0.9169792241116568 1102 15 1.695067362485382 1102 16 0.9382286554685066 1102 17 1.4215309926512418 1102 18 1.7359219154316463 1102 19 1.9521380545407894 1102 20 2.0686526798532014 1102 21 2.051730116270356 1102 22 2.1008549319126857 1102 23 1.8042290738061126 1102 24 1.8623068367891946 1102 25 1.8380119982068877 1103 1 0.19489969933441031 1103 2 0.45566150015570983 1103 3 -0.10149152829087527 1103 4 0.22063444860118153 1103 5 0.04315705698392236 1103 6 0.6421682445194108 1103 7 0.8088903068456261 1103 8 0.7988975351809628 1103 9 0.8182036484330695 1103 10 0.46256377952926897 1103 11 0.48514498500332337 1103 12 0.8307700925700416 1103 13 0.6826391704437498 1103 14 0.6880635614610568 1103 15 0.38479096276886793 1103 16 0.38953978232888886 1103 17 0.7567542385761143 1103 18 0.4273464370475182 1103 19 -0.024049046805771468 1103 20 0.14576111358813648 1103 21 0.40538000996276935 1103 22 0.7167301976816418 1103 23 0.336475707399309 1103 24 0.4172324214809038 1103 25 0.2570056197518866 1104 1 0.37494572613825383 1104 2 0.06646426176394016 1104 3 -0.0970242208588985 1104 4 -0.04523803007757721 1104 5 -0.535426941933089 1104 6 0.44113941008053104 1104 7 0.6013635706875107 1104 8 0.07532910415348226 1104 9 0.2715676663022961 1104 10 0.2919938593993123 1104 11 0.5536436989602791 1104 12 0.6718422463537195 1104 13 0.16673284852686265 1104 14 0.13046237017907458 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2.389506696639131 1107 15 2.5001093792921054 1107 16 2.527498209443948 1107 17 2.2515245285271277 1107 18 2.3824636090321647 1107 19 2.35950715602754 1107 20 2.517004328204848 1107 21 2.4466880994636284 1107 22 2.635437995907509 1107 23 2.6555542192489057 1107 24 2.102125841314077 1107 25 1.999227253539786 1108 1 0.42915631341961813 1108 2 0.22724267019669853 1108 3 0.031783841228442355 1108 4 0.026000241670367075 1108 5 0.3646078084433739 1108 6 0.2530240388306808 1108 7 0.32336055898822025 1108 8 0.25325103164102813 1108 9 0.6582235217407302 1108 10 0.8087319573953735 1108 11 0.4737178330967282 1108 12 0.3118283462031357 1108 13 0.5953208248966106 1108 14 -0.2685877861976209 1108 15 0.2158678476278908 1108 16 0.4665488495255161 1108 17 0.29842608058331876 1108 18 0.8768558147645749 1108 19 0.510294513000467 1108 20 0.5490650509872168 1108 21 0.35979561050928766 1108 22 0.423474701469992 1108 23 -0.3922536429919461 1108 24 -0.0794760619373065 1108 25 -0.8491923680757125 1109 1 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-1.597337486056226 2314 25 -1.3951845362208388 2315 1 -0.7441736673028707 2315 2 -0.5572682676961875 2315 3 -1.1287224030092595 2315 4 -0.3247666680605832 2315 5 -0.32779014646401095 2315 6 0.2740616718874236 2315 7 -0.6718619705566695 2315 8 -0.8324081754665045 2315 9 -1.265839842604251 2315 10 -0.48219084873990425 2315 11 -0.173587505936041 2315 12 -0.29901734988269624 2315 13 -0.1203777753883628 2315 14 -0.6114760604411913 2315 15 -0.3918766834256644 2315 16 -0.913134328749571 2315 17 -0.7219410303042864 2315 18 -0.52730142268409 2315 19 -0.629593174536204 2315 20 -1.0175261819354162 2315 21 -0.4255534800950258 2315 22 -0.36447266253973254 2315 23 -0.8734371146204722 2315 24 -0.7402756596240039 2315 25 -1.2900606942847475 2316 1 -1.6871057417849045 2316 2 -1.464742697279192 2316 3 -1.5868393712757154 2316 4 -1.2951836625531143 2316 5 -1.9434733355539677 2316 6 -4.542985272775574 2316 7 -2.9823261719728755 2316 8 -2.4512384928019415 2316 9 -2.5676494413454023 2316 10 -1.725621218527472 2316 11 -2.7267477472158745 2316 12 -2.212681131681798 2316 13 -2.575347615409342 2316 14 -2.4572143242754407 2316 15 -2.3284620492793238 2316 16 -2.526666980205114 2316 17 -1.945072422908531 2316 18 -2.616260247756209 2316 19 -2.51996820731879 2316 20 -2.6603795782113195 2316 21 -3.150546923577842 2316 22 -2.7902266620152147 2316 23 -3.5840062203115295 2316 24 -3.2348468634005627 2316 25 -3.3179652313946306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1194.0 3 0.0 4 24.0 5 215.0 6 270.0 7 201.0 8 151.0 9 133.0 10 133.0 11 125.0 12 115.0 13 107.0 14 114.0 15 108.0 16 119.0 17 115.0 18 113.0 19 136.0 20 202.0 21 235.0 22 309.0 23 373.0 24 463.0 25 492.0 26 635.0 27 709.0 28 786.0 29 1033.0 30 1337.0 31 1697.0 32 2108.0 33 2880.0 34 3793.0 35 5331.0 36 8057.0 37 15747.0 38 46621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.21915653740795 17.960816173483735 13.042527263069086 17.777500026039224 2 18.75630426870483 22.744241667966516 36.41865543596943 22.080798627359226 3 22.617772740977948 30.107817552323223 27.84333704161945 19.431072665079384 4 13.540377837157799 19.564561910604187 33.19747554039863 33.69758471183938 5 12.95727741062601 39.32767861789041 30.4480407972108 17.26700317427278 6 28.031327356312275 34.16990826451439 19.47766937782123 18.321095001352113 7 25.153393373406267 35.473907527194825 19.754986584579544 19.61771251481936 8 29.33743773204239 28.74673710702289 21.863202886945306 20.052622273989414 9 30.19717548201918 12.629214417937145 19.498117681316167 37.675492418727515 10 22.950768526799642 27.45481395203727 25.37282389400778 24.221593627155308 11 33.49973479766622 25.020540180753592 18.54024315413976 22.939481867440435 12 25.62398602271309 29.131827447065184 26.615083822122383 18.62910270809934 13 34.81271967223343 20.815048977809205 25.019237568371356 19.352993781586008 14 24.16195308600898 27.0639244718017 28.328273165862583 20.445849276326733 15 30.939904346017883 24.51861093782491 26.715533374922025 17.825951341235182 16 20.2597186554517 27.71441344964182 27.603164866241777 24.422703028664706 17 21.898295921231846 27.63955458978384 26.275459810149616 24.186689678834696 18 21.486572192012975 20.79308803193978 33.627223672035015 24.09311610401223 19 21.623813435085932 26.039446460319603 34.036867988480054 18.29987211611441 20 24.794917915180754 19.60470363169441 34.85407720859629 20.746301244528546 21 25.548705045695097 22.802840477849056 33.90898410288934 17.739470373566505 22 24.49444276936193 23.74377475800834 31.219263679936788 20.542518792692942 23 23.685551200341024 23.564945259458728 33.69376488079766 19.055738659402586 24 23.226000977324006 24.601532527214314 31.937700793295974 20.234765702165706 25 22.213326956467494 24.091036691238394 33.314272049573205 20.38136430272091 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 860.0 1 860.0 2 645.5 3 431.0 4 431.0 5 431.0 6 763.0 7 1095.0 8 1095.0 9 1095.0 10 988.0 11 881.0 12 881.0 13 881.0 14 544.5 15 208.0 16 208.0 17 208.0 18 533.5 19 859.0 20 859.0 21 859.0 22 1436.0 23 2013.0 24 2013.0 25 2013.0 26 3490.5 27 4968.0 28 4968.0 29 4968.0 30 5178.0 31 5388.0 32 5388.0 33 5388.0 34 5781.0 35 6174.0 36 6174.0 37 6174.0 38 5193.0 39 4212.0 40 4212.0 41 4212.0 42 5666.0 43 7120.0 44 7120.0 45 7120.0 46 12752.0 47 18384.0 48 18384.0 49 18384.0 50 17220.5 51 16057.0 52 16057.0 53 16057.0 54 13162.0 55 10267.0 56 10267.0 57 10267.0 58 8333.5 59 6400.0 60 6400.0 61 6400.0 62 6363.0 63 6326.0 64 6326.0 65 6326.0 66 4315.5 67 2305.0 68 2305.0 69 2305.0 70 1884.0 71 1463.0 72 1463.0 73 1463.0 74 1013.0 75 563.0 76 563.0 77 563.0 78 370.5 79 178.0 80 178.0 81 178.0 82 100.0 83 22.0 84 22.0 85 22.0 86 14.5 87 7.0 88 7.0 89 7.0 90 3.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17882949854960958 2 0.016635302190661356 3 0.0 4 0.0020794127738326695 5 0.09981181314396816 6 0.03638972354207172 7 0.023913246899075702 8 0.02495295328599204 9 0.023913246899075702 10 0.023913246899075702 11 0.029111778833657374 12 0.02599265967290837 13 0.015595595803745022 14 0.005198531934581674 15 0.0010397063869163347 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 96181.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.765411047920068 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25191815856778 18.04202493215916 2 9.526854219948849 4.337655046214949 3 3.297405918889295 2.252004034060781 4 1.374680306905371 1.2518064898472672 5 0.8494702228717574 0.9669269398321914 6 0.6804895871392035 0.9294975099032033 7 0.49780781877968583 0.7932959732171634 8 0.37906466934599925 0.6903650409124463 9 0.28315674095725246 0.5801561638993149 >10 2.5164413591523567 12.483754587704432 >50 0.6941907197661673 10.992815628866408 >100 0.5845816587504566 28.65742714257494 >500 0.03653635367190354 6.008463209989499 >1k 0.02740226525392766 12.01380730081825 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3238 3.3665692808350927 No Hit GGTATCAACGCAGAGTACTTTTTTT 2618 2.721951320946965 No Hit TATCAACGCAGAGTACTTTTTTTTT 2329 2.4214761751281437 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1221 1.2694814984248448 No Hit GTACTGGTTCACTATCGGTCAGTCA 1132 1.176947629989291 No Hit GTACATGGGAAGCAGTGGTATCAAC 1017 1.0573813954939126 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 975 1.0137137272434265 No Hit GGATACCACGTGTCCCGCCCTACTC 802 0.8338445223069005 No Hit CCCATGTACTCTGCGTTGATACCAC 765 0.7953753859909962 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 751 0.7808194965741674 No Hit ATCATTAACTGAATCCATAGGTTAA 729 0.7579459560620081 No Hit ATACAGGGTGACAGCCCCGTACACA 623 0.6477370790488766 No Hit GGTTAATGAGGCGAACCGGGGGAAC 617 0.6414988407273786 No Hit GGTATCAACGCAGAGTACATGGGGG 517 0.5375282020357451 No Hit GTACATGGGGGTTAAGCGACTAAGC 496 0.5156943679105022 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 490 0.5094561295890041 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 489 0.5084164232020878 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 480 0.4990590657198407 No Hit GTATTTAGCCTTGGAGGATGGTCCC 476 0.4949002401721754 No Hit GAGTACATGGGAAGCAGTGGTATCA 472 0.4907414146245101 No Hit CCCATATTCAGACAGGATACCACGT 449 0.46682816772543434 No Hit AGCGTACACGGTGGATGCCCTGGCA 423 0.4397958016656096 No Hit GTGGTATCAACGCAGAGTACTTTTT 422 0.4387560952786933 No Hit GTTAATGATAGTGTGTCGAAACACA 399 0.4148428483796176 No Hit TATTCAGACAGGATACCACGTGTCC 397 0.412763435605785 No Hit GCCTTGGAGGATGGTCCCCCCATAT 395 0.41068402283195227 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 387 0.4023663717366216 No Hit CGGTACTGGTTCACTATCGGTCAGT 383 0.3982075461889562 No Hit CTATCGGTCAGTCAGGAGTATTTAG 371 0.38573106954596026 No Hit GTATCAACGCAGAGTACATGGGGGT 367 0.38157224399829487 No Hit GTGGTATCAACGCAGAGTACATGGG 365 0.3794928312244622 No Hit GTTCACTATCGGTCAGTCAGGAGTA 361 0.37533400567679687 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 357 0.37117518012913153 No Hit TCACGGTACTGGTTCACTATCGGTC 356 0.3701354737422152 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 352 0.3659766481945499 No Hit ATCAACGCAGAGTACTTTTTTTTTT 339 0.3524604651646375 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 335 0.3483016396169722 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 333 0.34622222684313947 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 322 0.3347854565870598 No Hit TATCAACGCAGAGTACATGGGAAGC 318 0.3306266310393945 No Hit GGTATCAACGCAGAGTACATGGGAA 314 0.3264678054917291 No Hit GGTTCACTATCGGTCAGTCAGGAGT 311 0.32334868633098013 No Hit CATCTAAGTACCCCGAGGAAAAGAA 308 0.32022956717023116 No Hit GTTATAACCGGCGATTTCCGAATGG 304 0.31607074162256577 No Hit CATGTACTCTGCGTTGATACCACTG 303 0.31503103523564946 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 301 0.3129516224618168 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 301 0.3129516224618168 No Hit ACGCAGAGTACATGGGGGTTAAGCG 280 0.29111778833657376 No Hit GGTATCAACGCAGAGTACATGGGGA 277 0.2879986691758248 No Hit GATATGAACCGTTATAACCGGCGAT 276 0.2869589627889084 No Hit GTGCTAATCTGCGATAAGCGTCGGT 270 0.28072072446741037 No Hit GCGTTGATACCACTGCTTCCCATGT 268 0.2786413116935777 No Hit GTACTTAGATGTTTCAGTTCCCCCG 263 0.27344277975899606 No Hit ACGCAGAGTACATGGGAAGCAGTGG 263 0.27344277975899606 No Hit TCATTAACCTATGGATTCAGTTAAT 260 0.2703236605982471 No Hit GTATCAACGCAGAGTACATGGGAAG 257 0.26720454143749806 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 250 0.2599265967290837 No Hit CACTATCATTAACTGAATCCATAGG 247 0.2568074775683347 No Hit TATCAACGCAGAGTACATGGGGGTT 246 0.25576777118141836 No Hit GGAGTATTTAGCCTTGGAGGATGGT 235 0.2443310009253387 No Hit GAGTACATGGGGGTTAAGCGACTAA 228 0.23705305621692435 No Hit GGTGATATGAACCGTTATAACCGGC 228 0.23705305621692435 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 220 0.22873540512159365 No Hit CACACACACTGATTCAGGCTCTGGG 213 0.22145746041317932 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 212 0.22041775402626296 No Hit TCGGTAAGGTGATATGAACCGTTAT 210 0.2183383412524303 No Hit ATCCATAGGTTAATGAGGCGAACCG 199 0.20690157099635065 No Hit GGGTGACAGCCCCGTACACAAAAAT 198 0.2058618646094343 No Hit GCCCTACTCATCGAGCTCACAGCAT 196 0.20378245183560162 No Hit CCTATGGATTCAGTTAATGATAGTG 189 0.19650450712718728 No Hit GTGGTATCCTGTCTGAATATGGGGG 188 0.19546480074027092 No Hit CTATCATTAACTGAATCCATAGGTT 186 0.19338538796643828 No Hit GGGTACTTAGATGTTTCAGTTCCCC 185 0.19234568157952195 No Hit GGGAAACCCAGTGTGTTTCGACACA 181 0.1881868560318566 No Hit CCGTTATAACCGGCGATTTCCGAAT 178 0.1850677368711076 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 178 0.1850677368711076 No Hit ACTCTGCGTTGATACCACTGCTTCC 176 0.18298832409727495 No Hit AGCTCACAGCATGTGCATTTTTGTG 173 0.17986920493652592 No Hit GTCCCGCCCTACTCATCGAGCTCAC 171 0.17778979216269325 No Hit TTTGTGTACGGGGCTGTCACCCTGT 167 0.17363096661502792 No Hit TAAGTACCCCGAGGAAAAGAAATCA 166 0.17259126022811158 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 166 0.17259126022811158 No Hit ATTTAGCCTTGGAGGATGGTCCCCC 161 0.1673927282935299 No Hit GGGTTTCCCCATTCGGAAATCGCCG 160 0.16635302190661358 No Hit GCGTACACGGTGGATGCCCTGGCAG 157 0.16323390274586455 No Hit ATGATAGTGTGTCGAAACACACTGG 156 0.16219419635894822 No Hit CATTAACTGAATCCATAGGTTAATG 155 0.1611544899720319 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 152 0.15803537081128288 No Hit GGGAAGCAGTGGTATCAACGCAGAG 152 0.15803537081128288 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 150 0.15595595803745022 No Hit GGTATCAACGCAGAGTACATGGGCA 148 0.15387654526361755 No Hit GTCCCCCCATATTCAGACAGGATAC 148 0.15387654526361755 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 142 0.14763830694211955 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 141 0.1465986005552032 No Hit GAATATGGGGGGACCATCCTCCAAG 141 0.1465986005552032 No Hit GGAGGATGGTCCCCCCATATTCAGA 141 0.1465986005552032 No Hit GAGTAGGGCGGGACACGTGGTATCC 139 0.14451918778137054 No Hit TTCCAGACGCTTCCACTAACACACA 139 0.14451918778137054 No Hit CCCTGGCAGTCAGAGGCGATGAAGG 135 0.14036036223370518 No Hit CAACGCAGAGTACTTTTTTTTTTTT 135 0.14036036223370518 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 135 0.14036036223370518 No Hit GTACATGGGCATCTAAGTACCCCGA 134 0.13932065584678885 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 133 0.13828094945987252 No Hit CTACTGGGGGAATCTCGGTTGATTT 133 0.13828094945987252 No Hit GCTTCCCATGTACTCTGCGTTGATA 133 0.13828094945987252 No Hit GTGCATTTTTGTGTACGGGGCTGTC 131 0.13620153668603988 No Hit TAATGATAGTGTGTCGAAACACACT 129 0.13412212391220718 No Hit GTCAGAGGCGATGAAGGACGTGCTA 128 0.13308241752529085 No Hit CGTGGTATCCTGTCTGAATATGGGG 127 0.1320427111383745 No Hit GTCTGAATATGGGGGGACCATCCTC 123 0.12788388559070918 No Hit CGGTGGATGCCCTGGCAGTCAGAGG 122 0.12684417920379285 No Hit CGTCTGGAAAGGCGCGCGATACAGG 121 0.1258044728168765 No Hit AAGTACCCCGAGGAAAAGAAATCAA 121 0.1258044728168765 No Hit GTGTTTCGACACACTATCATTAACT 119 0.12372506004304384 No Hit GTATCGCGCGCCTTTCCAGACGCTT 118 0.12268535365612752 No Hit CAGTGGTATCAACGCAGAGTACATG 118 0.12268535365612752 No Hit CATTAACCTATGGATTCAGTTAATG 116 0.12060594088229484 No Hit GCTCTGGGCTGCTCCCCGTTCGCTC 116 0.12060594088229484 No Hit GTACGGGGCTGTCACCCTGTATCGC 115 0.11956623449537851 No Hit GCAGTGGTATCAACGCAGAGTACAT 114 0.11852652810846218 No Hit AACGCAGAGTACTTTTTTTTTTTTT 113 0.11748682172154584 No Hit CTACTCATCGAGCTCACAGCATGTG 112 0.1164471153346295 No Hit CTGTCACCCTGTATCGCGCGCCTTT 112 0.1164471153346295 No Hit GTATCAACGCAGAGTACATGGGGAA 112 0.1164471153346295 No Hit CTGTGAGCTCGATGAGTAGGGCGGG 110 0.11436770256079683 No Hit CCACGTGTCCCGCCCTACTCATCGA 110 0.11436770256079683 No Hit AGGTTAATGAGGCGAACCGGGGGAA 110 0.11436770256079683 No Hit TCAACGCAGAGTACTTTTTTTTTTT 109 0.1133279961738805 No Hit GAGCAGCCCAGAGCCTGAATCAGTG 109 0.1133279961738805 No Hit CACTGATTCAGGCTCTGGGCTGCTC 107 0.11124858340004784 No Hit TATTTAGCCTTGGAGGATGGTCCCC 106 0.11020887701313148 No Hit ATTCAGACAGGATACCACGTGTCCC 106 0.11020887701313148 No Hit AACACACACACTGATTCAGGCTCTG 106 0.11020887701313148 No Hit CAGGGTGACAGCCCCGTACACAAAA 105 0.10916917062621515 No Hit CTAACACACACACTGATTCAGGCTC 105 0.10916917062621515 No Hit ACCCAGTGTGTTTCGACACACTATC 104 0.10812946423929883 No Hit CATGGGAAGCAGTGGTATCAACGCA 104 0.10812946423929883 No Hit GCAGAGTACATGGGAAGCAGTGGTA 103 0.10708975785238249 No Hit GGGGAAACCCAGTGTGTTTCGACAC 102 0.10605005146546616 No Hit ATACCACTGCTTCCCATGTACTCTG 102 0.10605005146546616 No Hit ACATGGGAAGCAGTGGTATCAACGC 101 0.10501034507854982 No Hit AATCTGCGATAAGCGTCGGTAAGGT 101 0.10501034507854982 No Hit AAAATGCACATGCTGTGAGCTCGAT 100 0.10397063869163348 No Hit GATGAAGGACGTGCTAATCTGCGAT 100 0.10397063869163348 No Hit GACTAAGCGTACACGGTGGATGCCC 100 0.10397063869163348 No Hit GGGGTTAAGCGACTAAGCGTACACG 98 0.10189122591780081 No Hit AACCCAGTGTGTTTCGACACACTAT 97 0.10085151953088448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATGG 65 3.3036886E-6 14.610984 19 GATAAGC 40 0.00524206 14.245709 13 GGGGCTG 75 1.4492314E-5 12.662853 10 CCGAATG 75 1.4492314E-5 12.662853 18 TTTCCGA 80 2.8100912E-5 11.871425 15 CGGGGCT 80 2.8100912E-5 11.871425 9 TTCCGAA 80 2.8100912E-5 11.871425 16 ATTAACC 90 7.199358E-6 11.6196995 3 CCATGTA 225 0.0 11.408433 2 AATCCAT 185 0.0 11.293896 12 GAATCCA 185 0.0 11.293896 11 TGAATCC 190 0.0 10.996689 10 TACTCTG 245 0.0 10.853874 7 TTAACTG 195 1.8189894E-12 10.725877 5 ACTGAAT 195 1.8189894E-12 10.714722 8 TAACTGA 195 1.8189894E-12 10.714722 6 GGTATCA 790 0.0 10.595622 1 TACGGGG 90 9.331538E-5 10.552377 7 TCCGAAT 90 9.331538E-5 10.552377 17 GGGCTGT 90 9.331538E-5 10.552377 11 >>END_MODULE