FastQCFastQC Report
Thu 26 May 2016
SRR1512730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT90440.2844916206459633No Hit
CTGTAGGACGTGGAATATGGCAAGA79160.2490088090483686No Hit
GTCCTAAAGTGTGTATTTCTCATTT76980.2421513153176278No Hit
GTATCAACGCAGAGTACTTTTTTTT65910.20732908797849894No Hit
CTTTAGGACGTGAAATATGGCGAGG65300.2054102479896219No Hit
GTCCTACAGTGTGCATTTCTCATTT49080.15438797812144936No Hit
GGTATCAACGCAGAGTACTTTTTTT39740.12500770681634876No Hit
TATCAACGCAGAGTACTTTTTTTTT36660.11531913769218284No Hit
CTGTAGGACCTGGAATATGGCGAGA36290.1141552511415525No Hit
CTGAAGGACCTGGAATATGGCGAGA33970.1068573679051678No Hit
GTACATGGGAAGCAGTGGTATCAAC33850.10647989118604444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG603.0838753E-413.0988971
TAGGACC17000.013.0030794
GTGTAGG6950.011.8738211
GGCGAGG20650.011.7493219
GTCCTAT4850.011.5459351
AGGACGT48700.011.0457015
TGTAGGA54200.010.9750742
GGACGTG48600.010.892746
TCCAACG2350.010.88506218
ACCGTCC700.001549268610.8083458
AGGACCT32850.010.8012865
GTCCTAC46200.010.7385441
TGGCGAG51100.010.73476818
GTAGGAC53850.010.694053
AATACGG1252.2740824E-710.62922314
GGACCTG32950.010.5957496
TTAGGAC27450.010.5067993
GACGTGA26100.010.50501257
CTGTAGG48050.010.4477681
CCAACGA1553.814421E-910.39465419
Produced by FastQC (version 0.11.5)