FastQCFastQC Report
Thu 26 May 2016
SRR1512730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT92140.28983920750021075No Hit
CTGTAGGACGTGGAATATGGCAAGA92000.28939881799456685No Hit
GTATCAACGCAGAGTACTTTTTTTT85180.26794555779105655No Hit
GTCCTAAAGTGTGTATTTCTCATTT79770.2509276490372456No Hit
CTTTAGGACGTGAAATATGGCGAGG75670.23803052780053124No Hit
TATCAACGCAGAGTACTTTTTTTTT55980.17609288947104187No Hit
GGTATCAACGCAGAGTACTTTTTTT53300.1676625760772871No Hit
GTCCTACAGTGTGCATTTCTCATTT52430.16492586986364283No Hit
CTGTAGGACCTGGAATATGGCGAGA43080.13551414216528196No Hit
CTGAAGGACCTGGAATATGGCGAGA41910.1318337441538293No Hit
GTACATGGGAAGCAGTGGTATCAAC36980.11632574227651177No Hit
ATTTAGAAATGTCCACTGTAGGACG32490.1022018217026465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT1600.013.65536
TAGGACC16250.013.6293844
GTACGAC751.4712381E-512.6737633
TACGACC550.0030564512.0978734
AATCCCG3200.011.86875819
GGCGAGG21300.011.81303519
GTCCTAT5400.011.1038791
TCCAACG1900.010.99421818
TGTAGGA50950.010.8601182
AGGACCT31650.010.7526915
ACCGTGC1605.456968E-1010.6860848
CTGTAGG50400.010.6695431
TAGAACG1252.2248605E-710.6461284
GTAGGAC50550.010.5865413
ACCGTCA909.534138E-510.5541578
AGGACGT50400.010.48702055
CACCTTT12500.010.48314714
GGACGTG49250.010.4348076
TTAGGAC29800.010.4303363
ACCTTTT13000.010.37163215
Produced by FastQC (version 0.11.5)