FastQCFastQC Report
Thu 26 May 2016
SRR1512729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623754
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87050.3317765308790382No Hit
GTCCTACAGTGGACATTTCTAAATT77340.29476848820430573No Hit
GTCCTAAAGTGTGTATTTCTCATTT75940.28943262211320114No Hit
CTGTAGGACGTGGAATATGGCAAGA71040.27075709079433513No Hit
CTTTAGGACGTGAAATATGGCGAGG62290.23740792772493155No Hit
GGTATCAACGCAGAGTACTTTTTTT57640.2196852296366199No Hit
TATCAACGCAGAGTACTTTTTTTTT53660.204516124606194No Hit
GTCCTACAGTGTGCATTTCTCATTT38540.1468887708222646No Hit
CTGAAGGACCTGGAATATGGCGAGA32280.12302982672918268No Hit
GTCCTTCAGTGTGCATTTCTCATTT28760.10961393484297688No Hit
ATTTAGAAATGTCCACTGTAGGACG27840.10610750855453675No Hit
CTGTAGGACCTGGAATATGGCGAGA26570.10126711574332044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG350.002191612516.2623885
TAGGACC10850.012.5028044
GTTTAGG3450.012.2409671
TCTTACG701.1061482E-412.1967912
ATATCGA550.003102528612.0735916
GTCCTAA23750.011.5373731
CTAGGAC3900.011.432323
GTGTAGG3450.011.1022721
GGCGAGG19000.011.07582319
GGACGTA600.00594050311.0674596
GGTATCA20450.011.0458941
TCCAACG1900.010.97204918
ATCCTAC3150.010.9124891
AGGACCT24400.010.8860255
CCGGACC700.001509935410.8415924
GTCTAGG2400.010.6396771
AAATGTC10500.010.3898597
GGACCTG24800.010.3661796
ATGTCGA2750.010.35375317
TAGGACA12400.010.3279284
Produced by FastQC (version 0.11.5)