FastQCFastQC Report
Thu 26 May 2016
SRR1512729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623754
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110750.42210512113559423No Hit
CTGTAGGACGTGGAATATGGCAAGA83320.31756025907916674No Hit
GTCCTACAGTGGACATTTCTAAATT81280.3097851399178429No Hit
GTCCTAAAGTGTGTATTTCTCATTT77850.2967122679946367No Hit
TATCAACGCAGAGTACTTTTTTTTT77630.2958737747517488No Hit
GGTATCAACGCAGAGTACTTTTTTT75740.28867035552875764No Hit
CTTTAGGACGTGAAATATGGCGAGG74020.28211486290254345No Hit
CTGAAGGACCTGGAATATGGCGAGA41560.15839899624736162No Hit
GTCCTACAGTGTGCATTTCTCATTT40500.154358983349811No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35620.13575967868938935No Hit
ATTTAGAAATGTCCACTGTAGGACG31540.1202094403667417No Hit
CTGTAGGACCTGGAATATGGCGAGA31090.1184943405517438No Hit
TTTCTAAATTTTCCACCTTTTTCAG29540.11258677452230657No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28800.10976638815986559No Hit
GTCCTTCAGTGTGCATTTCTCATTT28710.10942336819686602No Hit
GAATATGGCAAGAAAACTGAAAATC27110.10332523552131793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC500.001493991713.3071174
CGTGCGC802.0038751E-613.06076910
TAGGACC11750.012.6195154
GCGTGCG853.9520837E-612.2924899
GTGCGCC1052.7271017E-711.76034611
TTACACG657.9907046E-411.6985654
GGTATCA23800.011.6772941
CCACCTT10500.011.66899213
GGCGAGG20150.011.35623219
AATGTCC12650.011.3384198
TATGTCG2100.011.303516
ATGTCGA2450.011.2389117
AAATGTC12000.011.1610217
GTCGAGG1855.456968E-1210.7780419
AGGACGT47700.010.6811735
GGACGTG46800.010.676756
TAGAAAT12750.010.66060454
TGTAGGA45700.010.6506472
ACCTTTT11100.010.6069615
GACGTGA25600.010.4634577
Produced by FastQC (version 0.11.5)