FastQCFastQC Report
Thu 26 May 2016
SRR1512728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166445
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80680.37240733090385403No Hit
GTATCAACGCAGAGTACTTTTTTTT80640.3722226966297321No Hit
GTCCTAAAGTGTGTATTTCTCATTT75260.3473893867603378No Hit
CTGTAGGACGTGGAATATGGCAAGA66750.3081084449409055No Hit
CTTTAGGACGTGAAATATGGCGAGG61900.2857215392036262No Hit
GGTATCAACGCAGAGTACTTTTTTT51610.23822437218576978No Hit
TATCAACGCAGAGTACTTTTTTTTT48640.22451527733221938No Hit
GTCCTACAGTGTGCATTTCTCATTT42840.19774330758454517No Hit
CTGAAGGACCTGGAATATGGCGAGA32090.148122846414287No Hit
CTGTAGGACCTGGAATATGGCGAGA27960.12905935761120177No Hit
GTCCTTCAGTGTGCATTTCTCATTT26680.12315106083930125No Hit
GAATATGGCAAGAAAACTGAAAATC26320.12148935237220423No Hit
ATTTAGAAATGTCCACTGTAGGACG25520.11779666688976642No Hit
TTTCTAAATTTTCCACCTTTTTCAG23500.10847263604661092No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23400.10801105036130619No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23050.10639550046273964No Hit
GGAATATGGCGAGAAAACTGAAAAT21920.10117958221879622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA400.005329194514.2300836
TAGGACC13350.014.1412554
GTGTAGG4800.013.4992521
GGCGAGG18600.012.4829119
GGTATCA17150.012.3078251
GCCGGTT1052.783927E-711.74137611
ACCTTTT8550.011.43402215
GTCTTAG2250.011.3448271
AAATGTC9850.011.2684927
CCTAGAC1604.7293724E-1111.2654833
ATAGGAC3800.010.9846263
CACCTTT9500.010.89007414
GACGTTA700.001509314310.8419677
TGTCGAG2100.010.82864518
AATGTCC10200.010.7558628
TGTAGGA45650.010.7232162
CGCCCTA2500.010.63080516
TAGAAAT11250.010.5408024
TTAGGAC26850.010.4936923
TGGCGAG44100.010.46339418
Produced by FastQC (version 0.11.5)