FastQCFastQC Report
Thu 26 May 2016
SRR1512728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166445
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103800.4791259413463069No Hit
CTGTAGGACGTGGAATATGGCAAGA80890.373376660842994No Hit
GTCCTACAGTGGACATTTCTAAATT80780.3728689165891587No Hit
TATCAACGCAGAGTACTTTTTTTTT75600.34895877809037384No Hit
GTCCTAAAGTGTGTATTTCTCATTT75020.3462815811156064No Hit
CTTTAGGACGTGAAATATGGCGAGG74710.3448506654911618No Hit
GGTATCAACGCAGAGTACTTTTTTT72540.3348342561200492No Hit
GTCCTACAGTGTGCATTTCTCATTT45470.20988301110805951No Hit
CTGAAGGACCTGGAATATGGCGAGA39790.183664944182751No Hit
CTGTAGGACCTGGAATATGGCGAGA35120.16210889267902023No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34030.1570776087091987No Hit
TTTCTAAATTTTCCACCTTTTTCAG29790.13750637565227827No Hit
ATTTAGAAATGTCCACTGTAGGACG29540.13635241143901644No Hit
GAATATGGCAAGAAAACTGAAAATC28130.12984405327621978No Hit
GTCCTTCAGTGTGCATTTCTCATTT27300.12601289208819055No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27130.12522819642317254No Hit
GGAATATGGCGAGAAAACTGAAAAT25330.11691965408768742No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22510.10390293776209411No Hit
ATTCCAGGTCCTTCAGTGTGCATTT21850.10085647223908292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGG250.00601016419.0123865
GTCGTAG350.00214982616.3091431
TATACGC350.002161142416.296335
TAGGACC13150.013.9504434
GGTATCA18950.012.7016241
TAGGTCG550.00305681812.0973934
CGGGAAT550.003072190812.08929310
AATGTCC9900.011.7055058
GGCGAGG20200.011.70450719
AAATGTC9550.011.339067
TCTAGGA3700.011.3057072
AGGACCT27750.011.1676355
GACCGGG600.005881762611.08210857
CTAGGAC3100.011.0381743
GGACCTG26850.011.0042866
GATATAC4250.010.96869851
TGTAGGA46050.010.9419052
TAGAAAT10550.010.9015784
CTGTAGG45500.010.7263981
GAAATGT10550.010.7161876
Produced by FastQC (version 0.11.5)