FastQCFastQC Report
Thu 26 May 2016
SRR1512727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200666
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58170.48448111298229485No Hit
GGTATCAACGCAGAGTACTTTTTTT43640.3634649436229559No Hit
TATCAACGCAGAGTACTTTTTTTTT37150.30941160988984445No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18830.15682962622411228No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17780.14808447978038855No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13510.11252088424257871No Hit
GTCCTACAGTGGACATTTCTAAATT12990.10818995457521077No Hit
GTCCTAAAGTGTGTATTTCTCATTT12100.10077740187529255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC551.1412516E-515.5261183
GAACCGC400.005321909714.2322766
GGTATCA10500.013.8376871
TAGGACC2900.012.7599724
GATCAAC1258.385541E-1012.5661141
CTAGATA550.003096455212.075874
CCCGTTT658.1365975E-411.67238412
GTGCGCC907.6028246E-611.5908411
GCTCAAG1052.3350913E-611.2197461
TGCGCCA855.4134456E-511.15742612
GTCCTAA3450.011.0977911
CGAAATC600.005927121311.07001113
GTACCGT600.005928970411.0695476
TTAATCA951.37822935E-510.9863184
GTTATAT906.812584E-510.9080871
TTTACAC1158.95041E-710.7257733
GTCTAGG650.00834083610.5724531
GACCGTG909.63065E-510.5424267
TAACACC909.63065E-510.5424264
GATATAT854.8702973E-410.3947641
Produced by FastQC (version 0.11.5)