FastQCFastQC Report
Thu 26 May 2016
SRR1512727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200666
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74420.6198226650875431No Hit
GGTATCAACGCAGAGTACTTTTTTT55770.46449220682521203No Hit
TATCAACGCAGAGTACTTTTTTTTT52250.4351751444614906No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25220.2100500888673453No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19450.16199342698135868No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16940.14108836262540955No Hit
GTCCTACAGTGGACATTTCTAAATT13560.11293731978751792No Hit
GTCCTAAAGTGTGTATTTCTCATTT12460.10377573779885498No Hit
CTTTAGGACGTGAAATATGGCGAGG12450.10369245068986713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCG400.00525119714.261015
GGTATCA12200.013.9696851
ACTGTTC2750.012.4340468
TAGGACC3100.012.2670244
TATACCT752.0590414E-411.4083334
GTCCTAA3300.010.9638491
CCCGTAC1158.8864726E-710.73176316
TAAGGTA803.7439584E-410.6953124
TCCTATA909.411905E-510.5663562
TATAAGA1451.418266E-810.49348452
CCAGTAC1451.4248144E-810.4904213
CACTGTT2900.010.4816797
CCCCGTA1106.090013E-610.35652615
TTATAAG750.002624457210.1437022
GTATCAA25550.010.1361121
GAACTGT1606.157279E-910.0939716
TCTACAC1701.5352271E-910.0661753
CGATAAG856.604447E-410.05652912
TGCGTTG856.604447E-410.05652912
TAGGGCG951.6310423E-410.0073094
Produced by FastQC (version 0.11.5)