##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512727_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200666
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.789613431212345	33.0	31.0	34.0	30.0	34.0
2	31.90948023846765	34.0	31.0	34.0	30.0	34.0
3	31.998882286997382	34.0	31.0	34.0	30.0	34.0
4	35.45661990928368	37.0	35.0	37.0	33.0	37.0
5	35.24216476522197	37.0	35.0	37.0	33.0	37.0
6	35.1012129934553	37.0	35.0	37.0	32.0	37.0
7	35.09661554503917	37.0	35.0	37.0	32.0	37.0
8	35.09930655153057	37.0	35.0	37.0	32.0	37.0
9	36.70650538950882	39.0	37.0	39.0	33.0	39.0
10	36.529142992305935	39.0	37.0	39.0	32.0	39.0
11	36.63252061772383	39.0	37.0	39.0	32.0	39.0
12	36.57426461647119	39.0	37.0	39.0	32.0	39.0
13	36.57019937268149	39.0	37.0	39.0	32.0	39.0
14	37.834352767547344	40.0	38.0	41.0	32.0	41.0
15	37.802001555803194	40.0	38.0	41.0	32.0	41.0
16	37.769796096499775	40.0	38.0	41.0	32.0	41.0
17	37.755852168713034	40.0	38.0	41.0	32.0	41.0
18	37.69854813911613	40.0	38.0	41.0	32.0	41.0
19	37.7226272751956	40.0	38.0	41.0	32.0	41.0
20	37.68024246543168	40.0	38.0	41.0	32.0	41.0
21	37.64533184082834	40.0	38.0	41.0	32.0	41.0
22	37.53762078712981	40.0	38.0	41.0	32.0	41.0
23	37.48520654370158	40.0	38.0	41.0	32.0	41.0
24	37.43775704484011	40.0	38.0	41.0	32.0	41.0
25	37.36310597618322	40.0	38.0	41.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5347996765982437
1101	2	0.48423445172057455
1101	3	0.5187973310812453
1101	4	0.4659077353344827
1101	5	0.5821314846009571
1101	6	0.6436409482665297
1101	7	0.612908520111354
1101	8	0.5585964973418598
1101	9	0.5203996017897623
1101	10	0.7915344006922354
1101	11	0.6365016262295029
1101	12	0.6486116697104123
1101	13	0.8707292630223975
1101	14	0.9059066425605877
1101	15	0.7806067141720376
1101	16	0.8623804727849134
1101	17	0.8406018374757522
1101	18	0.9414220327717757
1101	19	0.8662273133708069
1101	20	0.8376396709114857
1101	21	0.8389687250927906
1101	22	0.946752841325079
1101	23	0.9429040377786251
1101	24	0.8867288800996249
1101	25	0.7920934088756084
1102	1	0.4832972703527538
1102	2	0.401076173898268
1102	3	0.4943571248228622
1102	4	0.3593334795507417
1102	5	0.5174492410649592
1102	6	0.6917766070741251
1102	7	0.5880644577178842
1102	8	0.5846536637152298
1102	9	0.532393207656888
1102	10	0.6571885094293464
1102	11	0.5789912841341831
1102	12	0.49428488099627543
1102	13	0.7810662909483597
1102	14	0.813298438292307
1102	15	0.7717203436255886
1102	16	0.8348291922692113
1102	17	0.8329769415109354
1102	18	0.8110571188100266
1102	19	0.8269265833586132
1102	20	0.8085021417739569
1102	21	0.8038524360239307
1102	22	0.8718843632945479
1102	23	0.9506709743993227
1102	24	0.769835562613423
1102	25	0.6817907778219876
1103	1	0.37071546388824217
1103	2	0.4402099420512471
1103	3	0.4002477458297591
1103	4	0.3444805367402637
1103	5	0.49968515479733355
1103	6	0.6519122752482147
1103	7	0.5298749222786014
1103	8	0.4576161410760946
1103	9	0.6167885898277987
1103	10	0.6912694436619518
1103	11	0.5697384035466158
1103	12	0.5881581462547771
1103	13	0.5964003502156814
1103	14	0.6216490866328428
1103	15	0.6610191136119212
1103	16	0.6542160743456762
1103	17	0.7346952939370723
1103	18	0.9308390052754305
1103	19	0.8002290644972945
1103	20	0.7415458422595123
1103	21	0.6764815763477117
1103	22	0.6851295104628434
1103	23	0.7469937113186162
1103	24	0.7794578917504111
1103	25	0.8635761309925769
1104	1	0.35459863155407945
1104	2	0.34883378373744733
1104	3	0.3621707271497385
1104	4	0.2519824811839655
1104	5	0.35692690460957266
1104	6	0.5122871853761595
1104	7	0.4403543379031092
1104	8	0.4020511565648235
1104	9	0.5516777899464742
1104	10	0.6760513318704682
1104	11	0.6300168265025476
1104	12	0.6111493554982985
1104	13	0.5400288558939934
1104	14	0.6115633707434611
1104	15	0.733067768805256
1104	16	0.6778968595000165
1104	17	0.8986887402931458
1104	18	0.8230459956507659
1104	19	0.8251406624691882
1104	20	0.7907101992032466
1104	21	0.8218872100225383
1104	22	0.8536740241915552
1104	23	0.736804425403875
1104	24	0.6031529762501862
1104	25	0.6901244026427236
1105	1	0.315453100792336
1105	2	0.2783478343507042
1105	3	0.22463561825924927
1105	4	0.23182045100796245
1105	5	0.2428777027634439
1105	6	0.463925129794724
1105	7	0.3799811603897396
1105	8	0.2886723757430971
1105	9	0.37424910157227487
1105	10	0.47197517204210016
1105	11	0.5410978274764204
1105	12	0.3682240615716097
1105	13	0.5033956397079109
1105	14	0.6142097695240025
1105	15	0.6295265343837215
1105	16	0.5616458538684768
1105	17	0.5190610709589123
1105	18	0.33364570489975875
1105	19	0.4702338862769011
1105	20	0.5385966087105274
1105	21	0.6056305587402733
1105	22	0.5960206389182332
1105	23	0.6590849860169925
1105	24	0.622701041909103
1105	25	0.6920015815110503
1106	1	0.29335031372920284
1106	2	0.25379808305257257
1106	3	0.20627610386512885
1106	4	0.15041155308860965
1106	5	0.19155699751339483
1106	6	0.35905159269336195
1106	7	0.275744853557903
1106	8	0.3009326861288599
1106	9	0.5223304922636203
1106	10	0.5491164478135104
1106	11	0.4385256019770125
1106	12	0.35470749722591677
1106	13	0.3980887860232656
1106	14	0.49461439253857264
1106	15	0.509206535548941
1106	16	0.5962383542144067
1106	17	0.34619324344075864
1106	18	0.6607196163283859
1106	19	0.5599012884893639
1106	20	0.5359930678950136
1106	21	0.5007861521655101
1106	22	0.4427904276490935
1106	23	0.4557284210886934
1106	24	0.5897616989481307
1106	25	0.6774727313657678
1107	1	0.5336981844580997
1107	2	0.5347700386413905
1107	3	0.41195259348721436
1107	4	0.41028783518856216
1107	5	0.5067610669718476
1107	6	0.7301004471639416
1107	7	0.514624884794685
1107	8	0.6021850496758248
1107	9	0.6321563399770795
1107	10	0.7059059285381721
1107	11	0.7746156535683184
1107	12	0.7403823237786256
1107	13	0.7066767316625899
1107	14	0.8401224783095671
1107	15	0.8443602067745886
1107	16	0.8888969875329167
1107	17	0.678069583386602
1107	18	0.8862318236191413
1107	19	0.8434907706601749
1107	20	0.9386257790234822
1107	21	0.9721271283872355
1107	22	1.0339844753068732
1107	23	0.9131375759240612
1107	24	0.81162238434964
1107	25	0.833439715823296
1108	1	0.153947015700318
1108	2	0.1568908535357849
1108	3	0.12436031665178149
1108	4	0.14211911354510676
1108	5	0.1704065399450414
1108	6	0.08524445980591366
1108	7	0.14506219568261258
1108	8	0.030548000300534284
1108	9	0.08369075549962446
1108	10	0.14580894546093504
1108	11	0.16786429097579258
1108	12	-0.00372230755755254
1108	13	-0.0652034324727282
1108	14	0.22951084756803652
1108	15	0.1402308697222594
1108	16	0.30110266261989693
1108	17	0.1682487326820592
1108	18	0.12941360446760797
1108	19	0.3125807001240446
1108	20	0.2581350814483869
1108	21	0.11513971450406757
1108	22	0.28571469677127936
1108	23	0.15585283926005644
1108	24	0.17173053756449974
1108	25	0.2375818656805464
1109	1	0.13454671819777175
1109	2	0.1471233752613017
1109	3	0.020801246738678003
1109	4	0.04915426581943194
1109	5	0.0631052011835962
1109	6	0.27379844085969296
1109	7	0.08186012882282512
1109	8	0.02390273293968903
1109	9	-0.06421498316787222
1109	10	0.036274771813396
1109	11	0.17482898212117703
1109	12	0.03183394391039229
1109	13	-0.057289864790895706
1109	14	-0.10643842041912421
1109	15	-0.1907015658841047
1109	16	-0.44518090427903445
1109	17	-0.13756196175150848
1109	18	-0.560353873806875
1109	19	-0.5413330241380763
1109	20	-0.4031379162811959
1109	21	-0.3027260961903693
1109	22	-0.10104174259300436
1109	23	-0.288700515472577
1109	24	-0.25577748717518034
1109	25	-0.2617777209474923
1110	1	0.22206972357857424
1110	2	0.26706717891125464
1110	3	0.13757838606348116
1110	4	0.15719343467542046
1110	5	0.13816498005095212
1110	6	0.2542758288533449
1110	7	0.2594299176650452
1110	8	0.13387955194529866
1110	9	0.38207395036589276
1110	10	0.47912786050528666
1110	11	0.2600552426755556
1110	12	0.3804019204203186
1110	13	0.38841406473888185
1110	14	0.3422787574001802
1110	15	0.2943572295471384
1110	16	0.33274854120347896
1110	17	0.3132755043550617
1110	18	0.4582049707961673
1110	19	0.3246797043352103
1110	20	0.3670965629893743
1110	21	0.10019330419457617
1110	22	0.3560273367702891
1110	23	0.38054518901891043
1110	24	0.3198203717438801
1110	25	0.24413497802270712
1111	1	0.015375511241064288
1111	2	0.10400842316919068
1111	3	-0.0014301604381117272
1111	4	-0.05295475481987211
1111	5	0.004871754231182024
1111	6	-0.037001634289929086
1111	7	-0.0016085633052469461
1111	8	-0.081257362607694
1111	9	-0.08118001175157019
1111	10	-0.0011486695027969063
1111	11	0.07956931331632688
1111	12	-0.07729083131170711
1111	13	-0.13758026442588545
1111	14	-0.0827383747925623
1111	15	-0.13134677912117354
1111	16	-0.10420181197918765
1111	17	-0.07096436117178939
1111	18	0.00839461649076867
1111	19	-0.13824061693444634
1111	20	-0.09061154681342032
1111	21	0.014161098849754694
1111	22	-0.015837630834774075
1111	23	-0.030104203172193422
1111	24	-0.15006933612443163
1111	25	-0.13447800812642896
1112	1	-0.1334361918677729
1112	2	-0.15901042512614083
1112	3	-0.17842227580464254
1112	4	-0.19301753578871939
1112	5	-0.2001737309608771
1112	6	-0.07872657041859554
1112	7	-0.27775879748346455
1112	8	-0.25091402793390927
1112	9	-0.2956376405996082
1112	10	-0.14067026971726904
1112	11	-0.30389776468772567
1112	12	-0.16927955554560015
1112	13	-0.1361577893191992
1112	14	-0.27201759405179615
1112	15	-0.32552363419076613
1112	16	-0.09779667266265335
1112	17	-0.20260933059157082
1112	18	-0.15878021990458535
1112	19	-0.17188685477788823
1112	20	-0.3197570814219759
1112	21	-0.3825153910978827
1112	22	-0.2623229569336871
1112	23	-0.3267022669254871
1112	24	-0.25281965473084256
1112	25	-0.3744221494696376
1113	1	-0.21681596524038227
1113	2	-0.17242938504617555
1113	3	-0.23208484690212927
1113	4	-0.14533909546638313
1113	5	-0.18321205947329133
1113	6	0.013560844773046199
1113	7	-0.2120881590877275
1113	8	-0.22029013917770612
1113	9	-0.5215175687723459
1113	10	-0.3608181701852047
1113	11	-0.30631706959091076
1113	12	-0.37901161863328525
1113	13	-0.17983354054211986
1113	14	-0.3507710549404379
1113	15	-0.40383923185195414
1113	16	-0.23947859819936923
1113	17	-0.2009861406473874
1113	18	-0.32764947129115285
1113	19	-0.21577048046232505
1113	20	-0.1290079372455608
1113	21	-0.17093014930007655
1113	22	-0.2638090381904874
1113	23	-0.5099723533481892
1113	24	-0.40482011470837875
1113	25	-0.45392307384756236
1114	1	-0.49234879146864685
1114	2	-0.5458762502213759
1114	3	-0.5451311076838508
1114	4	-0.4839228460062728
1114	5	-0.47885546158292414
1114	6	-0.3669257981598406
1114	7	-0.7279821864690987
1114	8	-0.8802327131879295
1114	9	-0.9817075251073604
1114	10	-0.7884939960246058
1114	11	-0.7884987980242357
1114	12	-0.9173164183382667
1114	13	-1.0091899590659494
1114	14	-1.4812465771481698
1114	15	-1.202298688557896
1114	16	-1.2396421804791018
1114	17	-1.3070592045291392
1114	18	-1.2877956621916695
1114	19	-1.3718337150579671
1114	20	-1.2407001959294135
1114	21	-1.3462114318999454
1114	22	-1.1401795778828685
1114	23	-1.0390849768779233
1114	24	-1.1644474340970348
1114	25	-1.2952425227761708
1115	1	-0.5396417069340487
1115	2	-0.5323556921422963
1115	3	-0.6070673766016412
1115	4	-0.3798222922137242
1115	5	-0.8157258768927704
1115	6	-0.45755280747268756
1115	7	-0.6495953060288358
1115	8	-0.8578099917919033
1115	9	-0.698088307607236
1115	10	-0.5091696091026847
1115	11	-0.6299813857080565
1115	12	-0.66684593665628
1115	13	-0.7317806849420947
1115	14	-0.9453330268609008
1115	15	-0.9851110697275658
1115	16	-0.8447514525288256
1115	17	-0.9586635436078126
1115	18	-0.7827285641060939
1115	19	-0.7635247527448996
1115	20	-0.7334041001322902
1115	21	-0.9849063896914174
1115	22	-0.8972630754181097
1115	23	-0.939373280862327
1115	24	-1.0478288180944375
1115	25	-1.2069883174788458
1116	1	-0.7827354196775644
1116	2	-0.7621451561730055
1116	3	-0.7905376172703242
1116	4	-0.7293093810004265
1116	5	-0.7129523569986276
1116	6	-0.5252177840870758
1116	7	-0.5397589397722342
1116	8	-0.5745388890655434
1116	9	-0.7615622166064355
1116	10	-0.6327294618454147
1116	11	-0.6334440276921001
1116	12	-0.7535993331373092
1116	13	-0.7736415766084335
1116	14	-1.015175419660494
1116	15	-1.082070285752934
1116	16	-1.140570865857967
1116	17	-1.1485861156315522
1116	18	-1.3833967243073246
1116	19	-1.5339488379549735
1116	20	-1.4934533605706548
1116	21	-1.4337174575686333
1116	22	-1.5539492628435951
1116	23	-1.7476663280658826
1116	24	-1.719422838715694
1116	25	-1.5295524805099348
1201	1	0.5003188111364061
1201	2	0.6376224564279482
1201	3	0.6434558563709629
1201	4	0.5597177562944964
1201	5	0.5260902269291208
1201	6	0.6899044069083473
1201	7	0.6627476672591754
1201	8	0.6206415113462
1201	9	0.8086330363368646
1201	10	0.8105976553126055
1201	11	0.9189544529095599
1201	12	0.7695112059689393
1201	13	0.815144042889095
1201	14	1.131715179406207
1201	15	1.1131138668773133
1201	16	0.9519763526849943
1201	17	0.9332235043062411
1201	18	0.989508756057937
1201	19	0.9110473910654306
1201	20	0.8950313310032385
1201	21	0.9162400798253572
1201	22	1.0653031201278367
1201	23	0.8523296695677232
1201	24	0.9588350562647108
1201	25	1.020717866832932
1202	1	0.5178259986284921
1202	2	0.5318175103882297
1202	3	0.6764713695958662
1202	4	0.5946472123150457
1202	5	0.5199703458483285
1202	6	0.6342988163010403
1202	7	0.6696587644530112
1202	8	0.7092617986499121
1202	9	0.8003673183674422
1202	10	0.9820719056782039
1202	11	0.8736681494402063
1202	12	0.7777997867362672
1202	13	0.7778980955241508
1202	14	0.8198804970317113
1202	15	0.9333698158998018
1202	16	0.8672035476564872
1202	17	0.8431342822374361
1202	18	0.8838491520905905
1202	19	0.8926714172531334
1202	20	0.9919516304732312
1202	21	0.91491485407564
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2214	22	-1.5176327907294578
2214	23	-1.2911163929167202
2214	24	-1.3938716065496806
2214	25	-1.4141179798614232
2215	1	-0.2536396974696231
2215	2	-0.36475868946556744
2215	3	-0.27948786086722777
2215	4	-0.2140184680955528
2215	5	-0.23017254555355748
2215	6	-0.24658665390971635
2215	7	-0.4336975279442683
2215	8	-0.6067220432096434
2215	9	-0.6506879563344512
2215	10	-0.606854123188107
2215	11	-0.5693569177942948
2215	12	-0.5617294720865189
2215	13	-0.4888533094406853
2215	14	-0.45086672373855663
2215	15	-0.6435604074479784
2215	16	-0.6986219724585752
2215	17	-0.7088855817569524
2215	18	-0.7594341162102793
2215	19	-0.5411828830226142
2215	20	-0.597578110547758
2215	21	-0.5134308138878225
2215	22	-0.3067597122496011
2215	23	-0.6134442870517844
2215	24	-0.5099841604320119
2215	25	-0.4549577613480622
2216	1	-0.9900034807000786
2216	2	-0.9792788241718107
2216	3	-1.2465544458759439
2216	4	-0.9156056577843259
2216	5	-1.076661473998577
2216	6	-7.291767292751
2216	7	-3.2294219411746177
2216	8	-1.8126205083045477
2216	9	-1.686153355246752
2216	10	-1.4164838866297274
2216	11	-1.3500759483558085
2216	12	-1.3434560090110352
2216	13	-1.3769701684294517
2216	14	-1.5180817910990285
2216	15	-1.5329153537020659
2216	16	-1.560533385332036
2216	17	-1.5729339068143418
2216	18	-1.6623331203063927
2216	19	-1.6851667041740797
2216	20	-1.6878277694495765
2216	21	-1.533498695730529
2216	22	-1.5903786281081338
2216	23	-1.8395237214382973
2216	24	-1.792481013810466
2216	25	-1.861189907850651
2301	1	0.44718732051997634
2301	2	0.36763135784475764
2301	3	0.2852225094382419
2301	4	0.36555212503569123
2301	5	0.41967541163351996
2301	6	0.6477235134286374
2301	7	0.6105874510289269
2301	8	0.3717565065245978
2301	9	0.41058091668698893
2301	10	0.6337248806668043
2301	11	0.4568654480137795
2301	12	0.5488939947086493
2301	13	0.5152845183731074
2301	14	0.686417167389294
2301	15	0.6628679188764224
2301	16	0.7931799447775703
2301	17	0.8108197508754813
2301	18	0.8466444933919632
2301	19	0.7464499947298506
2301	20	0.7592232956201102
2301	21	0.7811726701376429
2301	22	0.8397982428932096
2301	23	0.844117880753835
2301	24	0.9631884054227839
2301	25	1.0188518146112884
2302	1	0.1837751325423973
2302	2	0.24021159217995347
2302	3	0.21831280490437166
2302	4	0.1744622753158751
2302	5	0.23551054917809466
2302	6	0.37183042886419315
2302	7	0.1732546058939235
2302	8	0.2900098177828738
2302	9	0.21320286014088197
2302	10	0.3385214293168346
2302	11	0.3346115636531053
2302	12	0.2223768653148639
2302	13	0.07907399876027199
2302	14	0.29457432480141676
2302	15	0.27509251133694335
2302	16	0.23947547457889584
2302	17	0.32552937899571077
2302	18	0.3688601709263537
2302	19	0.3346122463854826
2302	20	0.31684396551312943
2302	21	0.25705461104441696
2302	22	0.4465704028524513
2302	23	0.24426274830350536
2302	24	0.2883603361410749
2302	25	0.2350228538369592
2303	1	0.09392501191984692
2303	2	0.20750607793190312
2303	3	0.09908992153047791
2303	4	0.15199469751042471
2303	5	0.0808740060171047
2303	6	0.3545668151898198
2303	7	0.1767234811082048
2303	8	0.1772492287905294
2303	9	0.2409792629316172
2303	10	0.26030695565319206
2303	11	0.3397569099153728
2303	12	0.22665662104768813
2303	13	0.2308760423094398
2303	14	0.32087746515604465
2303	15	0.21818758691491524
2303	16	0.17851875280274498
2303	17	0.33653390690042073
2303	18	0.507487522805512
2303	19	0.29409948332151004
2303	20	0.25360896256920995
2303	21	0.26425874712124653
2303	22	0.3627416399760577
2303	23	0.3490721490465205
2303	24	0.24297642817894172
2303	25	0.33749665816269925
2304	1	0.15665458936224752
2304	2	0.18212242233581577
2304	3	0.1841784146441512
2304	4	0.09189487950344244
2304	5	0.1765300153631273
2304	6	0.06769971777983841
2304	7	0.08306462663109926
2304	8	-0.10898844297035026
2304	9	-0.01862702814595707
2304	10	0.2851064851734151
2304	11	0.01648326075659412
2304	12	-0.037889707700053066
2304	13	-0.17959213275354102
2304	14	-0.21199969352756654
2304	15	0.021286236669737946
2304	16	-0.056535964313340514
2304	17	0.01406372911946363
2304	18	0.2586855773080501
2304	19	0.19506110584096348
2304	20	0.1740033072751217
2304	21	0.2181239133388857
2304	22	0.06704668889165077
2304	23	0.061636820147775495
2304	24	0.06092539065976865
2304	25	0.015233904743375604
2305	1	0.27646724101847653
2305	2	0.35495409097754305
2305	3	0.3022643014451205
2305	4	0.2601387709316896
2305	5	0.3614123583460227
2305	6	0.3451276281686475
2305	7	0.34310920650808185
2305	8	0.32235008194358983
2305	9	0.12775297298264832
2305	10	0.43790542428519785
2305	11	0.3240025881083213
2305	12	0.10126158173056155
2305	13	0.24373047883722876
2305	14	0.37719391857107354
2305	15	0.47291707014414897
2305	16	0.5310465648893015
2305	17	0.51435434477321
2305	18	0.6056891324121594
2305	19	0.48283267629409465
2305	20	0.18409303133132227
2305	21	0.3810464346412985
2305	22	0.4866477272077745
2305	23	0.7295054505862382
2305	24	0.6691134161662333
2305	25	0.6340618489903136
2306	1	0.2805426670964799
2306	2	0.33487565201212277
2306	3	0.19173666835624914
2306	4	0.20648883255893224
2306	5	0.25403456209843966
2306	6	0.3252250983821483
2306	7	0.21600006725884668
2306	8	0.20145325208672915
2306	9	0.04201318634041229
2306	10	0.39361763214609624
2306	11	0.46781483516508615
2306	12	0.40348771829275876
2306	13	0.32833925210091763
2306	14	0.4737692383952137
2306	15	0.3943635866701669
2306	16	0.2793512254200081
2306	17	0.3314241464798826
2306	18	0.4059613784300069
2306	19	0.3800615754568142
2306	20	0.40740324067505185
2306	21	0.3905608780835479
2306	22	0.46213023782135565
2306	23	0.2937559830361707
2306	24	0.36148722199083494
2306	25	0.46694458722328847
2307	1	0.25509684322618753
2307	2	0.2498304840197818
2307	3	0.25537923524256456
2307	4	0.19173667091150293
2307	5	0.2525170805068129
2307	6	0.2939588669305593
2307	7	0.2797648474277068
2307	8	0.2785443722733092
2307	9	0.24594641715037824
2307	10	0.3838303851021294
2307	11	0.24430711026718654
2307	12	0.44880338501713624
2307	13	0.37158747746221366
2307	14	0.541714731271199
2307	15	0.5354537291504826
2307	16	0.6026410032973644
2307	17	0.5030777940371394
2307	18	0.50152707316159
2307	19	0.38188459025480626
2307	20	0.44586477913659195
2307	21	0.657128416198816
2307	22	0.5348573253769899
2307	23	0.46613272584849597
2307	24	0.3328842216547443
2307	25	0.5077841924743893
2308	1	-0.022133289425632086
2308	2	-0.10449373928034333
2308	3	-0.061781181315446076
2308	4	-0.028812191203627435
2308	5	0.1520350658681764
2308	6	0.15684113192125437
2308	7	0.08306462663109926
2308	8	0.15995095096904066
2308	9	0.324807315288389
2308	10	0.22450042456735275
2308	11	0.11825093752426596
2308	12	0.07170625189590396
2308	13	0.17823615007473848
2308	14	0.07360636707849721
2308	15	0.10360946899297119
2308	16	0.16013070235332805
2308	17	0.14891221396794663
2308	18	0.13494820357067283
2308	19	0.23571767149752532
2308	20	0.4209730042448143
2308	21	0.3845380547530297
2308	22	0.20088507273003842
2308	23	0.3111317696427207
2308	24	0.5791072088415916
2308	25	0.3119510764605451
2309	1	-0.30901460255694246
2309	2	-0.2755417190783227
2309	3	-0.32410441363867903
2309	4	-0.19353441342585143
2309	5	-0.5972957422126299
2309	6	-0.41655533272520984
2309	7	-0.5094606258941567
2309	8	-0.39824349347540533
2309	9	-0.7423517756207048
2309	10	-0.43363088856396104
2309	11	-0.6023172442939142
2309	12	-0.8702886975990438
2309	13	-0.8736577893191964
2309	14	-1.1124289864568624
2309	15	-1.4525647734312699
2309	16	-1.3749450552224332
2309	17	-1.1559110183552832
2309	18	-1.5142689681464958
2309	19	-1.3485500052701482
2309	20	-1.229670935149123
2309	21	-1.0379619452469697
2309	22	-1.4472209878760225
2309	23	-0.9452571192461647
2309	24	-0.9720039022695204
2309	25	-0.7482635700040987
2310	1	-0.24183544444606042
2310	2	-0.18208432688119558
2310	3	-0.17038778119514575
2310	4	-0.1622253785182508
2310	5	-0.05003958204836323
2310	6	0.01945339417417813
2310	7	-0.18691442466212038
2310	8	-0.20364133741790624
2310	9	-0.3667177920478082
2310	10	-3.0947910528350064
2310	11	-1.383639112877077
2310	12	-0.7828194984204018
2310	13	-0.25422760451426996
2310	14	-0.21704397618992033
2310	15	-0.291278839139693
2310	16	-0.11027873078710826
2310	17	-0.26051574114789133
2310	18	-0.13584494350583753
2310	19	-0.2534216685145054
2310	20	-0.1154435224181185
2310	21	0.0038912375045683234
2310	22	-0.08302694270148692
2310	23	-0.12190496318866906
2310	24	0.05834517370583825
2310	25	0.07334063944148994
2311	1	-0.20509614926444897
2311	2	-0.18517227038000783
2311	3	-0.1972437704533796
2311	4	-0.2590742296586228
2311	5	-0.29559398111003077
2311	6	-0.21343320408814748
2311	7	-0.33178834411774005
2311	8	-0.2062009973038883
2311	9	-0.2729930466773638
2311	10	-0.15294941842613952
2311	11	-0.2475258966675753
2311	12	-0.2985390804474335
2311	13	-0.44779638043711856
2311	14	-0.2984971334706401
2311	15	-0.3267933339213158
2311	16	-0.23871611188398845
2311	17	-0.2828750306064336
2311	18	-0.4399083249611948
2311	19	-0.39415107724564535
2311	20	-0.30747147113687134
2311	21	-0.38900712135723126
2311	22	-0.4443304825161434
2311	23	-0.5249240411450913
2311	24	-0.41944647501071586
2311	25	-0.4687019314129799
2312	1	-0.5667986934660334
2312	2	-0.4230701281692326
2312	3	-0.20674645909322464
2312	4	-0.1033071406985755
2312	5	-0.15036392403081322
2312	6	-0.10183942363430276
2312	7	-0.12045494407597346
2312	8	-0.013896902566308711
2312	9	-0.1418120028934311
2312	10	-0.05311952492759531
2312	11	-0.18714678974846066
2312	12	-0.31883983396267723
2312	13	-0.3414134711373791
2312	14	-0.3850426228204924
2312	15	-0.2837011370676379
2312	16	-0.31406437340424986
2312	17	-0.48113829108255857
2312	18	-0.3684451045101298
2312	19	-0.2599420507246961
2312	20	-0.22722775333094347
2312	21	-0.2880471725196969
2312	22	-0.29793121514875054
2312	23	-0.38315484651889165
2312	24	-0.3871175386331558
2312	25	-0.31675788818591855
2313	1	-0.612351636304723
2313	2	-0.3385101915472397
2313	3	-0.9606230637274891
2313	4	-0.6857591025910352
2313	5	-0.4346869500279098
2313	6	-0.36753002189039563
2313	7	-0.22274659392257234
2313	8	-0.33622528743632785
2313	9	-0.4259596797059615
2313	10	-0.2174053112993093
2313	11	-0.5313736386100771
2313	12	-0.4315187153610367
2313	13	-0.44699260281831243
2313	14	-0.37029310723838904
2313	15	-0.5913014519392661
2313	16	-0.4202692115279376
2313	17	-0.3431801124938261
2313	18	-1.6970709219653273
2313	19	-1.0407391704566535
2313	20	-0.6325417166766556
2313	21	-0.5584015544832042
2313	22	-0.5697565118547061
2313	23	-0.4658565863864865
2313	24	-0.4849346305110842
2313	25	-0.6211476730236356
2314	1	-1.0115731425018453
2314	2	-1.0081897907036677
2314	3	-0.7968592345753187
2314	4	-0.598141851442378
2314	5	-0.6984011141134587
2314	6	-0.7372130462238289
2314	7	-0.9628490556462168
2314	8	-1.2214769645497796
2314	9	-1.0849722990366857
2314	10	-0.8455179409055589
2314	11	-1.0026168310707746
2314	12	-1.07957933120786
2314	13	-1.1492431887682315
2314	14	-1.5254524024348228
2314	15	-1.4611288230180506
2314	16	-1.726232934010305
2314	17	-1.7369922855729172
2314	18	-1.7550885273751433
2314	19	-1.3463530355731805
2314	20	-1.736051789143616
2314	21	-1.5889467937318216
2314	22	-1.494707213771342
2314	23	-1.3745890751690268
2314	24	-1.5237394394596038
2314	25	-1.369293184329166
2315	1	-0.6195633830447491
2315	2	-0.4249929385905169
2315	3	-0.49761051119965316
2315	4	-0.5620100231819478
2315	5	-0.5379054983101952
2315	6	-0.5478967961398453
2315	7	-0.725863064918542
2315	8	-0.7326642251827167
2315	9	-0.8453700683036303
2315	10	-0.7532650349054251
2315	11	-0.7884148052695252
2315	12	-0.9471484536966059
2315	13	-0.9791455941972487
2315	14	-1.1861789864568593
2315	15	-1.3499428222117587
2315	16	-1.1995792015638926
2315	17	-1.1070390671357728
2315	18	-0.9834153096099101
2315	19	-0.933428054050637
2315	20	-0.8072928863686357
2315	21	-0.7961021891494084
2315	22	-0.8871600122662713
2315	23	-1.036598582660801
2315	24	-0.8986197559280598
2315	25	-0.8276538139065366
2316	1	-1.0990682835385392
2316	2	-0.9801506147838452
2316	3	-0.9956381559699672
2316	4	-0.7139178490086735
2316	5	-0.8792052514150868
2316	6	-4.5140936762834905
2316	7	-2.0958409939923115
2316	8	-1.364401468935526
2316	9	-1.490641652921319
2316	10	-1.0855634039013822
2316	11	-1.1039046676067983
2316	12	-1.3363776546542567
2316	13	-1.2281823291964997
2316	14	-1.492659047806555
2316	15	-1.4832933010386355
2316	16	-1.5063407607439032
2316	17	-1.597828196269397
2316	18	-1.6873824650790112
2316	19	-1.5964181034296558
2316	20	-1.6181141866828668
2316	21	-1.9165355648788704
2316	22	-1.8833406197778615
2316	23	-1.8734626407185573
2316	24	-1.6884456200609321
2316	25	-1.9159552877955122
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9840.0
3	0.0
4	208.0
5	1641.0
6	2187.0
7	1836.0
8	1419.0
9	1281.0
10	1140.0
11	1122.0
12	1065.0
13	1094.0
14	1107.0
15	1081.0
16	1077.0
17	1194.0
18	1271.0
19	1522.0
20	1853.0
21	2426.0
22	3121.0
23	3978.0
24	4895.0
25	5730.0
26	6679.0
27	7575.0
28	8836.0
29	10813.0
30	13702.0
31	18211.0
32	23882.0
33	31503.0
34	42458.0
35	57754.0
36	86243.0
37	157198.0
38	683724.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.66427457827034	18.669133551271134	12.984176848265744	24.682415022192785
2	16.191079916233647	20.71934439666734	38.95227083788012	24.137304849218896
3	18.98321431605459	24.867948288699772	30.13685737748883	26.011980017756812
4	13.230797677539078	17.134604284608905	36.805058707021274	32.82953933083074
5	13.62690849002412	37.525001188051455	34.850593150320606	13.99749717160382
6	32.115188812400746	36.39985802200659	17.559528273117667	13.925424892475
7	27.682916872855838	31.892341759181043	21.972518107158386	18.45222326080473
8	26.00175950440626	35.335047295011904	20.41566625400512	18.247526946576713
9	26.827502553413407	14.408499492649668	19.93970112150249	38.82429683243443
10	16.3371450755878	28.181094136093026	33.26412991137408	22.217630876945098
11	34.48522936414239	22.16399375798269	23.94170229110029	19.409074586774622
12	23.49712480400178	24.673863960155497	30.949222922252233	20.87978831359049
13	29.5876408893628	21.50923434493215	25.690722336535014	23.212402429170034
14	21.90853161811376	21.750436884550865	27.271924607636862	29.06910688969852
15	23.94351650877786	29.587867743209006	23.758947356146837	22.7096683918663
16	22.780490689525642	27.240706391690413	26.8736512368018	23.105151681982147
17	22.15866405696914	27.767292716445258	27.349768875192602	22.724274351392996
18	22.126292223173564	26.28947464860525	29.36190910877829	22.222324019442897
19	23.667248293639723	26.13856603270715	28.06384848270319	22.130337190949938
20	24.01877293000129	25.975105462389593	28.27483103113691	21.731290576472205
21	23.62355559331238	25.72480606596672	27.88927145434284	22.76236688637806
22	23.852984069581787	26.46121838090582	27.653147146691943	22.03265040282045
23	23.25051263215582	26.277749182537026	28.04251973488047	22.42921845042668
24	23.479552181872393	25.900875014367024	28.239993470290653	22.379579333469923
25	23.162228296628705	26.106094450913076	28.39140943443056	22.340267818027662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	591.0
1	591.0
2	679.5
3	768.0
4	768.0
5	768.0
6	1687.5
7	2607.0
8	2607.0
9	2607.0
10	3271.5
11	3936.0
12	3936.0
13	3936.0
14	4855.5
15	5775.0
16	5775.0
17	5775.0
18	9758.0
19	13741.0
20	13741.0
21	13741.0
22	21168.0
23	28595.0
24	28595.0
25	28595.0
26	40809.0
27	53023.0
28	53023.0
29	53023.0
30	66956.5
31	80890.0
32	80890.0
33	80890.0
34	90824.5
35	100759.0
36	100759.0
37	100759.0
38	108622.5
39	116486.0
40	116486.0
41	116486.0
42	125661.0
43	134836.0
44	134836.0
45	134836.0
46	144508.5
47	154181.0
48	154181.0
49	154181.0
50	153083.0
51	151985.0
52	151985.0
53	151985.0
54	139774.0
55	127563.0
56	127563.0
57	127563.0
58	111544.5
59	95526.0
60	95526.0
61	95526.0
62	80271.0
63	65016.0
64	65016.0
65	65016.0
66	50800.0
67	36584.0
68	36584.0
69	36584.0
70	27046.0
71	17508.0
72	17508.0
73	17508.0
74	12210.5
75	6913.0
76	6913.0
77	6913.0
78	4690.5
79	2468.0
80	2468.0
81	2468.0
82	1574.5
83	681.0
84	681.0
85	681.0
86	435.0
87	189.0
88	189.0
89	189.0
90	116.0
91	43.0
92	43.0
93	43.0
94	22.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15433101295447693
2	0.015324828053763496
3	0.0
4	0.0035813456864773386
5	0.10186013429213453
6	0.03997781231416564
7	0.025985578004207664
8	0.027318171748013185
9	0.025985578004207664
10	0.028317617055867324
11	0.03439757601198001
12	0.03331484359513803
13	0.02132149990088834
14	0.00999445307854141
15	0.0026651874876110425
16	0.002415326160647507
17	0.0013325937438055213
18	0.0018323163977325917
19	9.161581988662959E-4
20	8.328710898784508E-5
21	0.0
22	5.830097629149156E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1200666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.36740253908614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79325790402342	50.0734994633522
2	14.41709375608237	18.559817544829986
3	4.111166640392303	7.938753541421812
4	1.5586884893779203	4.013149177153148
5	0.7109729882811906	2.2881742265556184
6	0.39944285360240145	1.5426659369512217
7	0.24072194494538865	1.0846252431204093
8	0.15588238152575037	0.8026995200335502
9	0.10139449731479734	0.5873850381518865
>10	0.4523439260874934	4.990136123393285
>50	0.03155503529196599	1.396375904552743
>100	0.023979836720949145	3.247476090213054
>500	0.0019443035299996918	0.823151740552062
>1k	0.0011665821179998148	1.1299355384448182
>5k	3.8886070599993835E-4	1.522154911274257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7442	0.6198226650875431	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5577	0.46449220682521203	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5225	0.4351751444614906	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2522	0.2100500888673453	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1945	0.16199342698135868	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1694	0.14108836262540955	No Hit
GTCCTACAGTGGACATTTCTAAATT	1356	0.11293731978751792	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1246	0.10377573779885498	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1245	0.10369245068986713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.328710898784508E-5	0.0	0.0	0.0
6	0.0	8.328710898784508E-5	0.0	0.0	0.0
7	0.0	8.328710898784508E-5	0.0	0.0	0.0
8	0.0	8.328710898784508E-5	0.0	0.0	0.0
9	0.0	8.328710898784508E-5	0.0	0.0	0.0
10	0.0	8.328710898784508E-5	0.0	0.0	0.0
11	0.0	8.328710898784508E-5	0.0	0.0	0.0
12	0.0	8.328710898784508E-5	0.0	0.0	0.0
13	0.0	8.328710898784508E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAACCG	40	0.005251197	14.26101	5
GGTATCA	1220	0.0	13.969685	1
ACTGTTC	275	0.0	12.434046	8
TAGGACC	310	0.0	12.267024	4
TATACCT	75	2.0590414E-4	11.408333	4
GTCCTAA	330	0.0	10.963849	1
CCCGTAC	115	8.8864726E-7	10.731763	16
TAAGGTA	80	3.7439584E-4	10.695312	4
TCCTATA	90	9.411905E-5	10.566356	2
TATAAGA	145	1.418266E-8	10.4934845	2
CCAGTAC	145	1.4248144E-8	10.490421	3
CACTGTT	290	0.0	10.481679	7
CCCCGTA	110	6.090013E-6	10.356526	15
TTATAAG	75	0.0026244572	10.143702	2
GTATCAA	2555	0.0	10.136112	1
GAACTGT	160	6.157279E-9	10.093971	6
TCTACAC	170	1.5352271E-9	10.066175	3
CGATAAG	85	6.604447E-4	10.056529	12
TGCGTTG	85	6.604447E-4	10.056529	12
TAGGGCG	95	1.6310423E-4	10.007309	4
>>END_MODULE