FastQCFastQC Report
Thu 26 May 2016
SRR1512726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815255
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT107960.38348213572127565No Hit
GTCCTAAAGTGTGTATTTCTCATTT99670.3540354248549421No Hit
CTGTAGGACGTGGAATATGGCAAGA94210.33464108935069825No Hit
GTATCAACGCAGAGTACTTTTTTTT81300.28878378690385065No Hit
CTTTAGGACGTGAAATATGGCGAGG79540.2825321329684167No Hit
GTCCTACAGTGTGCATTTCTCATTT57950.20584280997636095No Hit
GGTATCAACGCAGAGTACTTTTTTT51930.1844593118562972No Hit
TATCAACGCAGAGTACTTTTTTTTT49430.17557912160710132No Hit
CTGAAGGACCTGGAATATGGCGAGA42070.1494358415134686No Hit
CTGTAGGACCTGGAATATGGCGAGA39940.14186991942115368No Hit
GTCCTTCAGTGTGCATTTCTCATTT36830.130822962751154No Hit
ATTTAGAAATGTCCACTGTAGGACG35630.12656047143153995No Hit
GAATATGGCAAGAAAACTGAAAATC32690.11611736769848557No Hit
TTTCTAAATTTTCCACCTTTTTCAG31800.11295601996977184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG455.250533E-415.2795521
TAGGACC17500.013.6616484
GGTATCA19450.012.4739061
GTGTAGG5500.012.1442671
CCAACGT1052.8131763E-711.73229219
GGCGAGG23600.011.72464419
GCGTCGG907.703307E-611.57857218
ACAGCGC752.1624025E-411.3494298
ACCTTTT13400.011.33170815
TCCAACG2350.011.2874318
AGGACCT36100.011.1166445
GATATAC7350.011.0921531
AGCGTCG951.377196E-510.98838217
CGTCGGT951.399724E-510.97230519
GGACCTG35450.010.8922786
CCACCTT14050.010.87209813
TAGAACG1408.538336E-910.8425764
GAAATGT14800.010.8334196
TAGAAAT15700.010.8166794
CACCTTT13700.010.80647914
Produced by FastQC (version 0.11.5)