FastQCFastQC Report
Thu 26 May 2016
SRR1512726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815255
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA111050.3944580508692818No Hit
GTCCTACAGTGGACATTTCTAAATT110770.3934634695613719No Hit
GTATCAACGCAGAGTACTTTTTTTT102780.3650823815249418No Hit
GTCCTAAAGTGTGTATTTCTCATTT100960.3586176030235272No Hit
CTTTAGGACGTGAAATATGGCGAGG96270.3419583661160357No Hit
TATCAACGCAGAGTACTTTTTTTTT69740.2477217871915688No Hit
GGTATCAACGCAGAGTACTTTTTTT68220.24232263152005767No Hit
GTCCTACAGTGTGCATTTCTCATTT60350.214367792615589No Hit
CTGAAGGACCTGGAATATGGCGAGA51910.18438827033430363No Hit
CTGTAGGACCTGGAATATGGCGAGA47690.16939850919366095No Hit
ATTTAGAAATGTCCACTGTAGGACG40190.1427579384460733No Hit
TTTCTAAATTTTCCACCTTTTTCAG39570.1405556512642727No Hit
GAATATGGCAAGAAAACTGAAAATC37360.1327055630839835No Hit
GTCCTTCAGTGTGCATTTCTCATTT35050.1245002672937265No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33930.12052194206208673No Hit
GGAATATGGCGAGAAAACTGAAAAT32890.11682778291842125No Hit
ATTCCAGGTCCTTCAGTGTGCATTT28660.10180250101678179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2050.014.823029518
CCAACGA1450.014.4077319
AACACCG655.424422E-513.1618725
TAGGACC15750.012.8547724
ACCGTGC550.003073875312.0886148
GGTATCA22550.012.0268071
TAAGACG951.0337844E-612.0066814
CCACCTT13150.011.91640813
GTCGAGG2000.011.395205519
ACCTTTT13650.011.3395515
TAGAAAT15150.011.2306724
TAGTACG855.296077E-511.1826934
AAATGTC14450.011.1088157
GGCGAGG24750.011.04989519
CACCTTT14750.010.94512914
GGACCTG32450.010.8602296
TGTCCAC16000.010.80419910
TTCCACC14000.010.78723711
AGGACCT34750.010.6957315
TGTAGGA58450.010.636842
Produced by FastQC (version 0.11.5)