FastQCFastQC Report
Thu 26 May 2016
SRR1512725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697505
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79690.2954211391637829No Hit
GTCCTAAAGTGTGTATTTCTCATTT73040.2707687288809474No Hit
CTGTAGGACGTGGAATATGGCAAGA68050.2522701533454062No Hit
GTATCAACGCAGAGTACTTTTTTTT66530.24663531670932956No Hit
CTTTAGGACGTGAAATATGGCGAGG59120.21916548810845576No Hit
GGTATCAACGCAGAGTACTTTTTTT43670.1618903394062291No Hit
GTCCTACAGTGTGCATTTCTCATTT43080.15970313308038353No Hit
TATCAACGCAGAGTACTTTTTTTTT38940.1443556175058063No Hit
CTGAAGGACCTGGAATATGGCGAGA32400.12011099145321324No Hit
CTGTAGGACCTGGAATATGGCGAGA29180.10817403489520871No Hit
ATTTAGAAATGTCCACTGTAGGACG28180.10446690552936881No Hit
GTCCTTCAGTGTGCATTTCTCATTT27580.10224262790986485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12800.013.1924124
TCCAACG3400.012.82081618
CCAACGA2450.012.76726419
GGCGAGG19650.011.43235719
GGTATCA18550.011.1695731
TGTAGGA42950.010.4697782
AGGACCT27550.010.4336255
AGGACGT43900.010.3726795
TTAGGAC27400.010.31782153
GACGTGA23500.010.2538797
GGACGTG43400.010.2298756
GATATAC8500.010.1662731
CATTCGT1502.5336703E-810.110641519
GTGTAGG5550.010.0850931
GGACCTG27850.010.0487256
AATGTCC11300.010.0438278
TGTAGAA12200.010.0311842
GGTCTAC2450.010.0200981
TAGAAAT11950.010.0026914
TGGCGAG44000.09.97160518
Produced by FastQC (version 0.11.5)