FastQCFastQC Report
Thu 26 May 2016
SRR1512725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697505
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86840.3219271141295382No Hit
GTCCTACAGTGGACATTTCTAAATT83310.30884094746812335No Hit
CTGTAGGACGTGGAATATGGCAAGA80580.29872048429938036No Hit
GTCCTAAAGTGTGTATTTCTCATTT78070.2894155895911222No Hit
CTTTAGGACGTGAAATATGGCGAGG72670.2693970910155866No Hit
GGTATCAACGCAGAGTACTTTTTTT58700.217608493774803No Hit
TATCAACGCAGAGTACTTTTTTTTT58690.21757142248114458No Hit
GTCCTACAGTGTGCATTTCTCATTT46390.17197373128131366No Hit
CTGAAGGACCTGGAATATGGCGAGA40480.15006459672919978No Hit
CTGTAGGACCTGGAATATGGCGAGA35840.13286351647170255No Hit
ATTTAGAAATGTCCACTGTAGGACG31390.11636679079371492No Hit
TTTCTAAATTTTCCACCTTTTTCAG30740.11395715670591898No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29470.10924910241130228No Hit
GTCCTTCAGTGTGCATTTCTCATTT29250.1084335339508175No Hit
GAATATGGCAAGAAAACTGAAAATC28750.10657996926789756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG456.732737E-414.7869865
TAGGACC12800.013.292944
TCGTATG550.003052616712.0997922
ATAGTAC2000.011.4066813
CAACACG752.0636346E-411.406684
TCCAACG4100.011.34767718
ATCCCGT2700.011.25791810
GGACCTG25100.011.0512966
CCCGTTT2850.010.99787412
AGGACCT26200.010.9934875
GGTATCA21150.010.9330571
CCACCTT12450.010.83184113
GGCGAGG20100.010.81768119
GTACTAG1158.6368163E-710.7570011
CGTTTCC2650.010.74984814
CCAACGA3100.010.72015719
ACCTTTT12600.010.62535215
AATCCCG5550.010.60702119
CCGTTTC2700.010.55214613
GTATTAG1909.094947E-1210.5174721
Produced by FastQC (version 0.11.5)