FastQCFastQC Report
Thu 26 May 2016
SRR1512724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196988
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71000.3231697214550102No Hit
GTATCAACGCAGAGTACTTTTTTTT70190.3194828556186925No Hit
GTCCTAAAGTGTGTATTTCTCATTT68120.31006086514810277No Hit
CTGTAGGACGTGGAATATGGCAAGA63900.29085274930950916No Hit
CTTTAGGACGTGAAATATGGCGAGG57160.2601743842023716No Hit
GGTATCAACGCAGAGTACTTTTTTT46880.21338304988466028No Hit
TATCAACGCAGAGTACTTTTTTTTT41650.18957773096621375No Hit
GTCCTACAGTGTGCATTTCTCATTT36200.16477104107987844No Hit
CTGAAGGACCTGGAATATGGCGAGA30900.1406471041261946No Hit
GTCCTTCAGTGTGCATTTCTCATTT25850.11766108872692978No Hit
ATTTAGAAATGTCCACTGTAGGACG25750.11720592010516216No Hit
CTGTAGGACCTGGAATATGGCGAGA23890.10873978374028442No Hit
TTTCTAAATTTTCCACCTTTTTCAG23540.10714669356409776No Hit
GAATATGGCAAGAAAACTGAAAATC23370.1063729069070928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10900.012.7060114
TCCAACG2250.012.63469318
GGCGAGG20250.011.70280219
CCAACGA1552.7284841E-1111.6197519
GTCCACG600.00457763811.4612141
GTGCGCC1354.8639777E-911.23931611
GTGTAGG4750.010.9614011
CTAATAC1303.340938E-810.94512753
AATGTCC10250.010.7992488
CGTGCGC803.836719E-410.66783810
AGGACCT24100.010.6668235
AAATGTC10150.010.6542217
GGACCTG23300.010.585236
GCGACTA1355.777838E-810.54457916
TAAGCGA1355.8107616E-810.54047613
TTAGGAC28050.010.4833913
GTTCTAA3200.010.437891
TAGAAAT11050.010.3873794
GTACTAA2001.2732926E-1110.3150921
CACCTTT10150.010.28344714
Produced by FastQC (version 0.11.5)