FastQCFastQC Report
Thu 26 May 2016
SRR1512724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196988
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85220.3878946994703658No Hit
CTGTAGGACGTGGAATATGGCAAGA75110.3418771518096594No Hit
GTCCTACAGTGGACATTTCTAAATT71510.3254910814260251No Hit
GTCCTAAAGTGTGTATTTCTCATTT71200.32408005869854545No Hit
CTTTAGGACGTGAAATATGGCGAGG69300.31543185488496067No Hit
TATCAACGCAGAGTACTTTTTTTTT60820.2768335557590665No Hit
GGTATCAACGCAGAGTACTTTTTTT60420.275012881271996No Hit
GTCCTACAGTGTGCATTTCTCATTT38370.1746482001722358No Hit
CTGAAGGACCTGGAATATGGCGAGA37330.16991444650585255No Hit
TTTCTAAATTTTCCACCTTTTTCAG31100.14155744136972984No Hit
ATTTAGAAATGTCCACTGTAGGACG29800.13564024928675078No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29380.13372854107532678No Hit
CTGTAGGACCTGGAATATGGCGAGA28750.13086097875819075No Hit
GAATATGGCAAGAAAACTGAAAATC26990.12285001101508064No Hit
GTCCTTCAGTGTGCATTTCTCATTT25520.11615903227509662No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25110.11429284092584939No Hit
GGAATATGGCGAGAAAACTGAAAAT24950.1135645711310212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC250.00603949718.9963810
TAGGACC12800.013.3680914
CGAATGG853.9679326E-612.28785919
AAGTGCG550.003073522612.0886067
CACCTTT9750.011.68715314
GGTATCA20400.011.3344061
ACCTTTT10500.011.12366615
CCTAATC600.00584850811.0905643
AAATGTC9850.010.9928297
CCACCTT10050.010.86634913
TCCAACG2450.010.46477218
ATAGGAC3000.010.4568183
TTTCCAC10250.010.378509510
TAGAAAT11000.010.3703984
TTCCACC10900.010.36958411
AGGACCT26500.010.2956265
AATGTCC11200.010.2614378
GAAATGT10500.010.2234926
AGGACTG4000.010.2193955
TGTAGGA42200.010.1833872
Produced by FastQC (version 0.11.5)