##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512724_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2196988 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.745903482404092 33.0 31.0 34.0 30.0 34.0 2 31.885079481544732 34.0 31.0 34.0 30.0 34.0 3 31.98417879387598 34.0 31.0 34.0 30.0 34.0 4 35.42568097777502 37.0 35.0 37.0 33.0 37.0 5 35.213416732362674 37.0 35.0 37.0 33.0 37.0 6 35.06522657383654 37.0 35.0 37.0 32.0 37.0 7 35.06906956251013 37.0 35.0 37.0 32.0 37.0 8 35.0686612762564 37.0 35.0 37.0 32.0 37.0 9 36.67901963961569 39.0 37.0 39.0 33.0 39.0 10 36.48643643023995 39.0 37.0 39.0 32.0 39.0 11 36.59321762340077 39.0 37.0 39.0 32.0 39.0 12 36.54515363761659 39.0 37.0 39.0 32.0 39.0 13 36.54638259289536 39.0 37.0 39.0 32.0 39.0 14 37.80807678512582 40.0 38.0 41.0 32.0 41.0 15 37.800794087177536 40.0 38.0 41.0 32.0 41.0 16 37.76953401657178 40.0 38.0 41.0 32.0 41.0 17 37.74939599123891 40.0 38.0 41.0 32.0 41.0 18 37.690267311428194 40.0 38.0 41.0 32.0 41.0 19 37.720234247979505 40.0 38.0 41.0 32.0 41.0 20 37.685937292329314 40.0 38.0 41.0 32.0 41.0 21 37.64063709041652 40.0 38.0 41.0 32.0 41.0 22 37.546379861883636 40.0 38.0 41.0 32.0 41.0 23 37.493713666164766 40.0 38.0 41.0 32.0 41.0 24 37.465658892993495 40.0 38.0 41.0 32.0 41.0 25 37.399742738694975 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7210511769416392 1101 2 0.6752523767931713 1101 3 0.7012693813614206 1101 4 0.5511387723157881 1101 5 0.6614940608658841 1101 6 0.7897265831322855 1101 7 0.6645481421696502 1101 8 0.7429747282419115 1101 9 0.8674312650880296 1101 10 1.065732407204905 1101 11 1.0145300626511116 1101 12 1.009389160157042 1101 13 0.9000785678146599 1101 14 0.995999852208115 1101 15 1.0735648675916423 1101 16 1.1775702654511804 1101 17 1.1483689797996632 1101 18 1.167685904303525 1101 19 1.1418089445225732 1101 20 1.2443295204309166 1101 21 1.2211917176694769 1101 22 1.2374870116312948 1101 23 1.2573283556430752 1101 24 1.2981712028116803 1101 25 1.308552750485326 1102 1 0.4660038961665727 1102 2 0.5336886289985792 1102 3 0.47850419115496123 1102 4 0.3888693724953143 1102 5 0.4755455822080066 1102 6 0.559192178823622 1102 7 0.5233016630784277 1102 8 0.4383155212589074 1102 9 0.42839135113749194 1102 10 0.6660338124731169 1102 11 0.5820676705998551 1102 12 0.5498796111428916 1102 13 0.641075101073703 1102 14 0.7749835090007622 1102 15 0.8473754968670306 1102 16 0.813376747018637 1102 17 0.8457739539538736 1102 18 0.8265040396921393 1102 19 0.7808829068217662 1102 20 0.8079244843706235 1102 21 0.8681451642909508 1102 22 0.8568264736673825 1102 23 0.8820381708533986 1102 24 0.8281027965839272 1102 25 0.7971020586847928 1103 1 0.315157370680307 1103 2 0.3270504351052601 1103 3 0.3765051796962382 1103 4 0.3246629205231031 1103 5 0.4150252753425221 1103 6 0.5104630112867596 1103 7 0.39737403543826133 1103 8 0.4492329850239969 1103 9 0.5207712294494513 1103 10 0.679163311380151 1103 11 0.6409990017890266 1103 12 0.5698940911841106 1103 13 0.6032338106792636 1103 14 0.6782404740398107 1103 15 0.5489404551672976 1103 16 0.5922028194466904 1103 17 0.5677693454022901 1103 18 0.6140494638598852 1103 19 0.7349627280489486 1103 20 0.7420925085411554 1103 21 0.6654872413414807 1103 22 0.7287425033514694 1103 23 0.6910321560670525 1103 24 0.7223201782026294 1103 25 0.7630809233938649 1104 1 0.280220359312775 1104 2 0.35534070043730637 1104 3 0.3083487231933475 1104 4 0.21331858042482565 1104 5 0.24220987842002017 1104 6 0.3687041360741645 1104 7 0.2682238105748951 1104 8 0.18779486782614896 1104 9 0.3243258520890606 1104 10 0.4351159724313973 1104 11 0.3452440121078695 1104 12 0.22758667035684965 1104 13 0.17902100052579328 1104 14 0.24643138270293719 1104 15 0.3446164569688932 1104 16 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-1.6359565536309546 2314 19 -1.5976293395609034 2314 20 -1.7180589985059953 2314 21 -1.5870073429167633 2314 22 -1.6347660949743315 2314 23 -1.5912744150329488 2314 24 -1.7213320375712158 2314 25 -1.685215458088308 2315 1 -0.7671845226283907 2315 2 -0.6466075163743525 2315 3 -0.7143446347344913 2315 4 -0.5001730800673059 2315 5 -0.6128085933696994 2315 6 -0.5536456931977938 2315 7 -0.7832847620972245 2315 8 -0.7055195360246884 2315 9 -0.9917360556089889 2315 10 -0.9008361408138867 2315 11 -0.8191600978005695 2315 12 -1.084666887988611 2315 13 -1.0134832871056219 2315 14 -1.1978889953340257 2315 15 -1.3322010583292752 2315 16 -1.31286792360644 2315 17 -1.2721813444527967 2315 18 -1.1128146741750413 2315 19 -1.1333314041883682 2315 20 -1.1603731128305839 2315 21 -1.12087433037469 2315 22 -1.116860288632914 2315 23 -1.082498202274131 2315 24 -1.1540852864661701 2315 25 -1.21969438333943 2316 1 -1.299816792767846 2316 2 -1.1760449454350663 2316 3 -1.183946561918944 2316 4 -0.9972228824002158 2316 5 -1.1609476963916663 2316 6 -4.5068454882392075 2316 7 -2.3589674991709018 2316 8 -1.7635078606104457 2316 9 -1.788387176838171 2316 10 -1.5774580588979248 2316 11 -1.6188178626522998 2316 12 -1.6922253865846386 2316 13 -1.681503505648422 2316 14 -1.8800030384768007 2316 15 -1.9135685007802934 2316 16 -2.036844545152647 2316 17 -2.099098654182775 2316 18 -2.285503703711022 2316 19 -2.2264567383816285 2316 20 -2.238721559955863 2316 21 -2.492768574295063 2316 22 -2.4220979686058612 2316 23 -2.5847552436082424 2316 24 -2.441474755324336 2316 25 -2.522575809910677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18949.0 3 1.0 4 383.0 5 3303.0 6 4223.0 7 3353.0 8 2740.0 9 2338.0 10 2236.0 11 2061.0 12 1948.0 13 1911.0 14 1957.0 15 1996.0 16 2018.0 17 2173.0 18 2355.0 19 2785.0 20 3459.0 21 4468.0 22 5878.0 23 7293.0 24 8811.0 25 10569.0 26 11953.0 27 13771.0 28 15957.0 29 19652.0 30 25213.0 31 32610.0 32 42961.0 33 56839.0 34 76383.0 35 104856.0 36 157786.0 37 284981.0 38 1256818.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41622830551056 20.093663939474478 13.449432368080227 25.040675386934737 2 14.227534387825013 21.120213417340615 42.24934957332465 22.402902621509718 3 18.346208536414398 25.957492712750362 30.69083672737402 25.005462023461213 4 12.201963023637214 17.97245867731917 39.07216339989176 30.75341489915186 5 12.189906701482562 38.86684925115604 36.487570753008384 12.455673294353018 6 30.436857438707086 38.17427669307416 18.584313786204053 12.804552082014704 7 26.788003729712408 32.11263257962062 23.34284579970279 17.756517890964187 8 24.008107783253386 38.638677331351914 21.163372196786753 16.189842688607943 9 26.297151395755524 14.751396483988183 21.20306372811257 37.748388392143724 10 15.203819701493623 29.25639295538795 35.22638387690414 20.313403466214293 11 33.034101174345075 21.982135311944518 26.620097182154616 18.363666331555788 12 23.622394644066162 24.82897318345048 32.92994149687678 18.61869067560658 13 29.15127891349715 22.643943780980372 26.24690879159701 21.95786851392546 14 19.74071862066203 23.793224943598858 28.450438918336246 28.01561751740287 15 21.89673073675519 33.479371318989294 23.574536498192693 21.04936144606282 16 20.359485588212937 28.381727666543615 30.334691752123543 20.924094993119905 17 20.45224474635768 29.83997829720449 29.144651316915436 20.563125639522394 18 20.300693014099252 27.21107912195455 32.66147000921743 19.82675785472877 19 23.09662865499871 26.680221779488626 28.75745861207115 21.46569095344151 20 23.307340146910356 27.79398685289756 29.408152805707456 19.490520194484624 21 21.783323349968228 26.882486385906525 28.340345964566033 22.993844299559214 22 22.694665835218505 29.43945524973031 28.19474093865642 19.67113797639476 23 20.696471714911507 29.090099718341655 29.475308922943594 20.738119643803245 24 22.72152725528189 27.261199087659598 30.059303946723396 19.957969710335107 25 21.649139640271134 28.138615231398624 29.674172093793867 20.538073034536374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 346.0 1 346.0 2 608.0 3 870.0 4 870.0 5 870.0 6 2231.0 7 3592.0 8 3592.0 9 3592.0 10 4707.0 11 5822.0 12 5822.0 13 5822.0 14 8654.5 15 11487.0 16 11487.0 17 11487.0 18 20621.0 19 29755.0 20 29755.0 21 29755.0 22 48213.5 23 66672.0 24 66672.0 25 66672.0 26 98160.5 27 129649.0 28 129649.0 29 129649.0 30 167585.5 31 205522.0 32 205522.0 33 205522.0 34 224832.5 35 244143.0 36 244143.0 37 244143.0 38 252210.0 39 260277.0 40 260277.0 41 260277.0 42 267690.0 43 275103.0 44 275103.0 45 275103.0 46 281286.0 47 287469.0 48 287469.0 49 287469.0 50 267284.5 51 247100.0 52 247100.0 53 247100.0 54 210444.5 55 173789.0 56 173789.0 57 173789.0 58 145787.5 59 117786.0 60 117786.0 61 117786.0 62 95269.0 63 72752.0 64 72752.0 65 72752.0 66 55403.0 67 38054.0 68 38054.0 69 38054.0 70 27599.0 71 17144.0 72 17144.0 73 17144.0 74 11835.5 75 6527.0 76 6527.0 77 6527.0 78 4389.5 79 2252.0 80 2252.0 81 2252.0 82 1457.0 83 662.0 84 662.0 85 662.0 86 416.0 87 170.0 88 170.0 89 170.0 90 102.5 91 35.0 92 35.0 93 35.0 94 22.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15480284826316756 2 0.016067452348396988 3 0.0 4 0.003231697214550102 5 0.10036468109976021 6 0.04069207478602523 7 0.026035645165107868 8 0.026399780062521964 9 0.026354263200345198 10 0.026672881235582535 11 0.0318618035237334 12 0.0316342192128496 13 0.01984535190906823 14 0.01019577712759469 15 0.003231697214550102 16 0.003231697214550102 17 0.001638607038363432 18 0.002867562317136006 19 0.0014110227274796222 20 9.103372435352401E-5 21 0.0 22 8.19303519181716E-4 23 0.0 24 4.551686217676201E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2196988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.22477959703272 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66529914491329 53.918259170497464 2 11.859058963409543 15.470090142332058 3 2.881822274400771 5.638986680568295 4 1.0098831370156383 2.6347762012242 5 0.4798733920956395 1.5649818116959275 6 0.25660580014052914 1.0042234054491743 7 0.16172335867709028 0.7383859297783558 8 0.1081775119341152 0.5644683498606433 9 0.08300751710762926 0.4872732307417837 >10 0.4249739913848579 5.1191184056350085 >50 0.03370298841647481 1.519745103763243 >100 0.02867305808196277 3.9322440911774748 >500 0.003774160881680917 1.6871225094714826 >1k 0.0029354584635294463 3.470250855494662 >5k 4.892430772549076E-4 2.2500741123102506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8522 0.3878946994703658 No Hit CTGTAGGACGTGGAATATGGCAAGA 7511 0.3418771518096594 No Hit GTCCTACAGTGGACATTTCTAAATT 7151 0.3254910814260251 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7120 0.32408005869854545 No Hit CTTTAGGACGTGAAATATGGCGAGG 6930 0.31543185488496067 No Hit TATCAACGCAGAGTACTTTTTTTTT 6082 0.2768335557590665 No Hit GGTATCAACGCAGAGTACTTTTTTT 6042 0.275012881271996 No Hit GTCCTACAGTGTGCATTTCTCATTT 3837 0.1746482001722358 No Hit CTGAAGGACCTGGAATATGGCGAGA 3733 0.16991444650585255 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3110 0.14155744136972984 No Hit ATTTAGAAATGTCCACTGTAGGACG 2980 0.13564024928675078 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2938 0.13372854107532678 No Hit CTGTAGGACCTGGAATATGGCGAGA 2875 0.13086097875819075 No Hit GAATATGGCAAGAAAACTGAAAATC 2699 0.12285001101508064 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2552 0.11615903227509662 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2511 0.11429284092584939 No Hit GGAATATGGCGAGAAAACTGAAAAT 2495 0.1135645711310212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.5516862176762E-5 0.0 4 0.0 0.0 0.0 4.5516862176762E-5 0.0 5 0.0 0.0 0.0 4.5516862176762E-5 0.0 6 0.0 0.0 0.0 4.5516862176762E-5 0.0 7 0.0 0.0 0.0 4.5516862176762E-5 0.0 8 0.0 0.0 0.0 4.5516862176762E-5 0.0 9 0.0 0.0 0.0 9.1033724353524E-5 0.0 10 9.1033724353524E-5 0.0 0.0 9.1033724353524E-5 0.0 11 9.1033724353524E-5 0.0 0.0 9.1033724353524E-5 0.0 12 9.1033724353524E-5 0.0 0.0 9.1033724353524E-5 0.0 13 9.1033724353524E-5 0.0 0.0 1.3655058653028602E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCGC 25 0.006039497 18.99638 10 TAGGACC 1280 0.0 13.368091 4 CGAATGG 85 3.9679326E-6 12.287859 19 AAGTGCG 55 0.0030735226 12.088606 7 CACCTTT 975 0.0 11.687153 14 GGTATCA 2040 0.0 11.334406 1 ACCTTTT 1050 0.0 11.123666 15 CCTAATC 60 0.005848508 11.090564 3 AAATGTC 985 0.0 10.992829 7 CCACCTT 1005 0.0 10.866349 13 TCCAACG 245 0.0 10.464772 18 ATAGGAC 300 0.0 10.456818 3 TTTCCAC 1025 0.0 10.3785095 10 TAGAAAT 1100 0.0 10.370398 4 TTCCACC 1090 0.0 10.369584 11 AGGACCT 2650 0.0 10.295626 5 AATGTCC 1120 0.0 10.261437 8 GAAATGT 1050 0.0 10.223492 6 AGGACTG 400 0.0 10.219395 5 TGTAGGA 4220 0.0 10.183387 2 >>END_MODULE