FastQCFastQC Report
Thu 26 May 2016
SRR1512723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767735
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66430.3757916203503353No Hit
GGTATCAACGCAGAGTACTTTTTTT43420.24562505126616827No Hit
TATCAACGCAGAGTACTTTTTTTTT41810.23651735129982718No Hit
GTCCTACAGTGGACATTTCTAAATT33860.19154454711820493No Hit
GTCCTAAAGTGTGTATTTCTCATTT31360.17740215586612246No Hit
CTGTAGGACGTGGAATATGGCAAGA30150.17055723850011456No Hit
CTTTAGGACGTGAAATATGGCGAGG28460.1609969820137068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18820.10646392134567681No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18080.1022777735350604No Hit
GTCCTACAGTGTGCATTTCTCATTT17920.10137266049492712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT508.8029876E-515.1821017
GTACTAG707.998229E-512.6268981
GGTATCA13700.012.3299141
TAGGACC7150.011.54554
GTCCTAT2800.011.2239091
GGCGAGG9700.011.04015219
GAGCCGA700.001506659210.8440496
GTCTTAG2001.8189894E-1210.8030131
TAGTACC803.8134813E-410.6746124
GGCTTAG1302.4327346E-710.5763761
AGGACGT22200.010.5570735
TGTAGGA21400.010.5526792
GTCCTAA10000.010.5083851
GTATAAG2250.010.4756481
GGACGTG21950.010.4179466
AGGACCT12900.010.3711995
GTATTAA2750.010.35660651
GACGTGA12000.010.2795487
TTAGGAC14350.010.2489493
AAATGTC5250.010.1214017
Produced by FastQC (version 0.11.5)