FastQCFastQC Report
Thu 26 May 2016
SRR1512723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767735
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83210.47071535043431284No Hit
GGTATCAACGCAGAGTACTTTTTTT58280.32968742486854646No Hit
TATCAACGCAGAGTACTTTTTTTTT58080.32855603356837987No Hit
CTGTAGGACGTGGAATATGGCAAGA34930.19759749057409623No Hit
GTCCTACAGTGGACATTTCTAAATT33840.19143140798818825No Hit
GTCCTAAAGTGTGTATTTCTCATTT32490.18379451671206373No Hit
CTTTAGGACGTGAAATATGGCGAGG31970.1808528993316306No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25550.1445352385962828No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20020.1132522691466764No Hit
GTCCTACAGTGTGCATTTCTCATTT18590.10516282135048523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.013.1238881
TAGGACC6700.012.48531154
CTACACG657.982956E-411.6995224
TTAGGCT1001.9156105E-611.4070354
CGAGACG752.085715E-411.39316118
GGCGAGG9300.011.22981719
TCGAACT951.3694551E-510.99340119
CTGTAGG21350.010.9650021
TGGCGAG19350.010.941751518
TGTAGGA21250.010.9162032
GTAGGAC20900.010.5971453
GTCTTAT1803.45608E-1110.5737491
GCCTCGA909.568429E-510.5498216
GTCCTAA10350.010.3898571
AGGACGT21850.010.3547985
GGACGTG21300.010.3027566
TAGGGGT1201.5116548E-610.2980184
TTATACT2053.6379788E-1210.2014134
GACGTGG11100.010.1840477
TATGGCG20950.010.10667616
Produced by FastQC (version 0.11.5)