FastQCFastQC Report
Thu 26 May 2016
SRR1512722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092603
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75660.3615592637495024No Hit
GGTATCAACGCAGAGTACTTTTTTT50090.2393669511130396No Hit
TATCAACGCAGAGTACTTTTTTTTT47370.22636878567028723No Hit
GTCCTAAAGTGTGTATTTCTCATTT46200.22077766303498564No Hit
GTCCTACAGTGGACATTTCTAAATT45360.21676352370707677No Hit
CTGTAGGACGTGGAATATGGCAAGA41230.19702733867819172No Hit
CTTTAGGACGTGAAATATGGCGAGG39750.18995480748139998No Hit
GTCCTACAGTGTGCATTTCTCATTT27830.13299225892345562No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22200.10608796795187621No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21880.10455877201743474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT400.005312156414.23692117
ATTTCCG905.470047E-712.654736514
GCCGGTT1001.479093E-712.32680411
TAGGACC8500.012.2769284
TTAGACG701.1055885E-412.19720654
GCGCCAC1052.7660462E-711.74715313
GGTATCA14600.011.6401891
GACTGCG951.3819601E-510.9846197
TGGCGAA1900.010.97143118
TGCGCCA1053.5413213E-610.83805312
CGAATGG803.8507176E-410.66358419
CGGTTTC1252.2800123E-710.62665613
AATGTCC7850.010.5998768
GGTCTAG1302.4248766E-710.5791661
GTCCTAG2800.010.5251911
TTAGGAC17300.010.4737743
GTCCTAC24300.010.349051
GTCCTAA13800.010.3218061
GGCGAGG14700.010.31699719
AGGACGT30150.010.2261465
Produced by FastQC (version 0.11.5)