FastQCFastQC Report
Thu 26 May 2016
SRR1512722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092603
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94850.4532632324430386No Hit
GGTATCAACGCAGAGTACTTTTTTT66450.3175470932613592No Hit
TATCAACGCAGAGTACTTTTTTTTT65730.3141064024088659No Hit
GTCCTAAAGTGTGTATTTCTCATTT49880.2383634162810624No Hit
GTCCTACAGTGGACATTTCTAAATT48140.23004841338753698No Hit
CTGTAGGACGTGGAATATGGCAAGA47500.22699002151865405No Hit
CTTTAGGACGTGAAATATGGCGAGG46940.2243139286333815No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30030.14350548097274066No Hit
GTCCTACAGTGTGCATTTCTCATTT28580.1365763118948028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25510.12190558839875504No Hit
CTGAAGGACCTGGAATATGGCGAGA24620.11765251220608973No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21550.102981788710042No Hit
CTGTAGGACCTGGAATATGGCGAGA21380.10216940336986997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT402.7498632E-416.6343564
GGTATCA17600.011.8449581
ACCGCTC752.0765314E-411.398788
CCGCTCA752.0769924E-411.3985089
TAGGACC8150.011.3131114
AAGGCGT1950.011.2047346
CCACCTT8100.011.02087813
GGCGAGG13200.011.00518819
CACCTTT8200.010.76886214
CTAGTAC1158.7564877E-710.7451743
GCGCCAC1158.8595516E-710.73541613
TAGGACA8350.010.700634
AATCCCG1909.094947E-1210.49411219
TGTACTG3000.010.4563815
GTCCTAA15950.010.4443031
CTAGGAC3100.010.4252193
GTCGAGG1106.0879065E-610.35782319
CGAAATC1752.3646862E-1010.31071813
ATAGGAC3150.010.259743
GATATAC4850.010.2061921
Produced by FastQC (version 0.11.5)