FastQCFastQC Report
Thu 26 May 2016
SRR1512721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399239
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57310.2388674075404743No Hit
GGTATCAACGCAGAGTACTTTTTTT38850.16192634414495596No Hit
TATCAACGCAGAGTACTTTTTTTTT38260.1594672310678511No Hit
GTCCTACAGTGGACATTTCTAAATT38130.15892539259323477No Hit
GTCCTAAAGTGTGTATTTCTCATTT35170.14658814732504766No Hit
CTGTAGGACGTGGAATATGGCAAGA32120.13387578311289539No Hit
CTTTAGGACGTGAAATATGGCGAGG30230.12599828528962725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.012.4205431
TCCAACG1052.8144677E-711.73166518
TAGGACC8900.011.725384
GTCCTAC20700.010.8655291
ACGGTAC803.8191976E-410.6732073
GTACAAA3250.010.5771931
CCAGCGC1002.4355453E-510.4360273
TCCTACA24650.010.3917652
GTAGGAC24000.010.1988433
GGACGTG22700.010.1137856
TGTAGGA26200.010.1028842
GTGTAGG4200.010.0555291
AGGACCT16200.09.8382645
AGGACGT23250.09.8337325
CCTACAG24050.09.8226053
GTCCTAA11600.09.8216791
TAGGACA7300.09.7468144
GACGTGG10850.09.705497
GTATCAA28750.09.7021111
TTAGGAC15650.09.638853
Produced by FastQC (version 0.11.5)