FastQCFastQC Report
Thu 26 May 2016
SRR1512721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399239
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74070.30872289088331756No Hit
TATCAACGCAGAGTACTTTTTTTTT54230.22603000368033363No Hit
GGTATCAACGCAGAGTACTTTTTTT53370.2224455337713333No Hit
GTCCTACAGTGGACATTTCTAAATT38100.1588003529452464No Hit
CTGTAGGACGTGGAATATGGCAAGA36720.15304852913778078No Hit
GTCCTAAAGTGTGTATTTCTCATTT36710.15300684925511798No Hit
CTTTAGGACGTGAAATATGGCGAGG36210.15092285512197826No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24570.10240747170248567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.013.3322271
TAGGACC8950.011.5762854
GGCGAGG11250.011.47887619
TAGGTCG700.001484473410.8636525
AGGACCT15950.010.4294475
GTCCTAA12200.010.2165261
TGGCGAG24450.010.13639818
TTAGGAC15700.010.049563
AGGACGT23200.09.9973695
GGACGTG22650.09.9403426
GGACCTG16100.09.912996
TGCCGGT1554.0739906E-89.80517110
GTAGGAC23700.09.7854343
CCTATAC2151.0913936E-119.7257443
AATGTCC6850.09.7069648
TGTAGGA25000.09.6587932
GACGTGA13750.09.6025827
GTCCTAC21250.09.5370151
TATGGCG26000.09.53211316
TGGACCG1106.794151E-59.5056955
Produced by FastQC (version 0.11.5)