##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512721_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2399239 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68181660934988 33.0 31.0 34.0 30.0 34.0 2 31.817477541837224 34.0 31.0 34.0 30.0 34.0 3 31.912552271782843 34.0 31.0 34.0 30.0 34.0 4 35.36657248402514 37.0 35.0 37.0 33.0 37.0 5 35.141936255621054 37.0 35.0 37.0 32.0 37.0 6 34.99123096948657 37.0 35.0 37.0 32.0 37.0 7 34.98311131154504 37.0 35.0 37.0 32.0 37.0 8 34.978921649739775 37.0 35.0 37.0 32.0 37.0 9 36.580149372363486 39.0 37.0 39.0 32.0 39.0 10 36.39341599565529 39.0 37.0 39.0 32.0 39.0 11 36.504409106387484 39.0 37.0 39.0 32.0 39.0 12 36.44719888264571 39.0 37.0 39.0 32.0 39.0 13 36.43944934206221 39.0 37.0 39.0 32.0 39.0 14 37.70069842979378 40.0 38.0 41.0 32.0 41.0 15 37.682275504858 40.0 38.0 41.0 32.0 41.0 16 37.64844811208887 40.0 38.0 41.0 32.0 41.0 17 37.61926010705895 40.0 38.0 41.0 32.0 41.0 18 37.55139067012499 40.0 38.0 41.0 32.0 41.0 19 37.58223169930132 40.0 38.0 41.0 32.0 41.0 20 37.535850325874165 40.0 38.0 41.0 32.0 41.0 21 37.49201934446714 40.0 38.0 41.0 32.0 41.0 22 37.392720775212474 40.0 38.0 41.0 32.0 41.0 23 37.338010927631636 40.0 38.0 41.0 32.0 41.0 24 37.29242897435395 40.0 38.0 41.0 32.0 41.0 25 37.223262876270354 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5678571511672388 1101 2 0.5192175637497733 1101 3 0.4449289313376106 1101 4 0.47188652628571504 1101 5 0.5044415827580551 1101 6 0.7164369095975971 1101 7 0.6877654669249651 1101 8 0.6677239092261544 1101 9 0.7526378567905283 1101 10 0.8244416284387697 1101 11 0.819444458061561 1101 12 0.8591051119832542 1101 13 0.8328227873997633 1101 14 0.9587642623180201 1101 15 0.8841892955340498 1101 16 0.8730622295228727 1101 17 0.8999633480951204 1101 18 0.915499266392473 1101 19 0.9936599309292902 1101 20 0.9822796645279368 1101 21 1.0698586925817963 1101 22 1.039718424928573 1101 23 1.0596008076168886 1101 24 1.0525223023516688 1101 25 0.9492677881773375 1102 1 0.4108640681925735 1102 2 0.47354106802351836 1102 3 0.4011636468865767 1102 4 0.43960399099589864 1102 5 0.3601121860706087 1102 6 0.5549944029221336 1102 7 0.5258122592221994 1102 8 0.47724016679391923 1102 9 0.44323459536503407 1102 10 0.7073151326086773 1102 11 0.6179924434734474 1102 12 0.524290462472365 1102 13 0.5979556329144984 1102 14 0.6763839763971902 1102 15 0.7843192080578518 1102 16 0.8075458012809023 1102 17 0.7701595724569401 1102 18 0.8691285571070964 1102 19 0.81567297655576 1102 20 0.8261465605475067 1102 21 0.8621092906495207 1102 22 0.9660506041342245 1102 23 0.8083241966477885 1102 24 0.8551591055633665 1102 25 0.8555927590409453 1103 1 0.37958867844501754 1103 2 0.38987779598743444 1103 3 0.4328109644320115 1103 4 0.4114555940234794 1103 5 0.36002479413279076 1103 6 0.5249570087312421 1103 7 0.4631537759912092 1103 8 0.4659287142170214 1103 9 0.6500083581205018 1103 10 0.7250515994891771 1103 11 0.6425995894029626 1103 12 0.6134809332008473 1103 13 0.6226124488711733 1103 14 0.6814931616995068 1103 15 0.7155566027949476 1103 16 0.6468521687394215 1103 17 0.6238058986500761 1103 18 0.6179260645251645 1103 19 0.7245475937039672 1103 20 0.6989996547000317 1103 21 0.6149201703995786 1103 22 0.7060730734207397 1103 23 0.6827300338830327 1103 24 0.811482253639447 1103 25 0.8310899030997234 1104 1 0.3979789473549289 1104 2 0.3893020597974477 1104 3 0.3647577483653244 1104 4 0.35157903026473747 1104 5 0.2830788757178837 1104 6 0.3923244679020783 1104 7 0.37270061052406334 1104 8 0.2405067739910578 1104 9 0.4172867687623807 1104 10 0.5505218090112152 1104 11 0.3961287407288694 1104 12 0.43735209745626946 1104 13 0.37355220213184737 1104 14 0.38057987332874177 1104 15 0.4651686175220746 1104 16 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17 -1.965229945892844 2314 18 -1.9617589931521664 2314 19 -1.8815314962323342 2314 20 -1.951414227076846 2314 21 -1.829680178073474 2314 22 -1.721817516126194 2314 23 -1.8599772431530397 2314 24 -1.897953970750308 2314 25 -1.7916446018948662 2315 1 -0.5853069921129403 2315 2 -0.5810560477219369 2315 3 -0.6018885877852362 2315 4 -0.42930051538783687 2315 5 -0.5448299062729731 2315 6 -0.610538966476561 2315 7 -0.7839819245064703 2315 8 -0.8364027763348929 2315 9 -0.9739256956922162 2315 10 -0.8845396181910203 2315 11 -0.9517725955405041 2315 12 -1.2065356780948377 2315 13 -1.0673446112577807 2315 14 -1.370989668917808 2315 15 -1.4473283130980548 2315 16 -1.5120719708217294 2315 17 -1.401354961723122 2315 18 -1.2174188989037162 2315 19 -1.1498969455787957 2315 20 -1.1212927172354128 2315 21 -1.1121129077457326 2315 22 -1.104534190902399 2315 23 -1.1606911051397262 2315 24 -1.1597046989428534 2315 25 -1.3418415638155636 2316 1 -1.3530484160137846 2316 2 -1.1763459027944023 2316 3 -1.2090812877315606 2316 4 -1.0773522891642457 2316 5 -1.3115319508735723 2316 6 -4.742174158981321 2316 7 -2.6253423890553407 2316 8 -2.002993807418676 2316 9 -1.8838315171922417 2316 10 -1.6552588881856494 2316 11 -1.8135876055439226 2316 12 -1.9139430855022397 2316 13 -1.9401987565756897 2316 14 -2.154436455625465 2316 15 -2.1098740604264137 2316 16 -2.2230854876323463 2316 17 -2.2319434553550934 2316 18 -2.451775752463469 2316 19 -2.3483647150610523 2316 20 -2.550581986742074 2316 21 -2.6812889678638214 2316 22 -2.5849506745797513 2316 23 -2.679878144616616 2316 24 -2.591466764038252 2316 25 -2.5067612925034055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22826.0 3 2.0 4 452.0 5 3860.0 6 4946.0 7 3819.0 8 3062.0 9 2782.0 10 2549.0 11 2396.0 12 2311.0 13 2239.0 14 2244.0 15 2301.0 16 2317.0 17 2598.0 18 2695.0 19 3182.0 20 3956.0 21 5135.0 22 6581.0 23 8215.0 24 10125.0 25 12068.0 26 13714.0 27 15683.0 28 18132.0 29 22417.0 30 28694.0 31 37116.0 32 48781.0 33 63510.0 34 85760.0 35 116500.0 36 173035.0 37 313017.0 38 1350219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88215934749204 19.667367547505656 13.72929135061282 24.72118175438948 2 15.43163120892589 20.774532956210543 40.03514161905735 23.758694215806226 3 18.11278492888787 25.17998415330861 30.47387108995811 26.233359827845415 4 12.882009976858491 17.516647548800577 37.57381774893796 32.02752472540298 5 13.329403115969784 37.92918432552547 35.53896964887555 13.2024429096292 6 31.992858286522473 36.996519206906214 17.843567787903453 13.16705471866786 7 27.91510448303795 31.766237155629966 22.545889947507295 17.77276841382479 8 25.313044453458883 36.57009590093641 20.565108034991773 17.551751610612932 9 26.54500489240258 14.663074596736369 20.36619435282915 38.4257261580319 10 15.418407617129034 28.86315900064788 34.35391733657301 21.364516045650074 11 33.9834913074621 22.114960471028905 24.970168780910022 18.931379440598974 12 23.19629494357194 24.76009526144363 31.80563963756623 20.2379701574182 13 28.91114022804311 21.98817971140887 25.95840262269167 23.142277437856357 14 21.034926767128347 22.16494351166803 27.79101714012266 29.009112581080963 15 23.038986848774243 31.13533524955318 23.409490289967188 22.41618761170539 16 22.231030794713856 27.84532869174359 27.865418905975297 22.05822160756726 17 21.733740968362437 28.649865267869984 27.82630079547183 21.79009296829575 18 21.604505513101483 27.12744363315347 29.932468987501636 21.335581866243412 19 23.517939856061012 26.77530238065764 27.86115935613833 21.845598407143015 20 23.73882874479314 26.977065218206782 28.22158535334969 21.062520683650394 21 23.076150396021404 26.403663828405588 27.54473397606491 22.975451799508097 22 23.506286621246385 27.534425743011692 27.428933212516394 21.53035442322553 23 22.3991857418123 27.402772295715433 28.00250412735038 22.195537835121886 24 23.216682310535518 26.5875470358064 28.34881603977266 21.84695461388542 25 22.75321466515007 27.08979805680051 28.078611593092646 22.07837568495677 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 254.0 2 523.5 3 793.0 4 793.0 5 793.0 6 2029.5 7 3266.0 8 3266.0 9 3266.0 10 4328.5 11 5391.0 12 5391.0 13 5391.0 14 8323.0 15 11255.0 16 11255.0 17 11255.0 18 19843.0 19 28431.0 20 28431.0 21 28431.0 22 44960.0 23 61489.0 24 61489.0 25 61489.0 26 88783.0 27 116077.0 28 116077.0 29 116077.0 30 147742.5 31 179408.0 32 179408.0 33 179408.0 34 202757.0 35 226106.0 36 226106.0 37 226106.0 38 243714.0 39 261322.0 40 261322.0 41 261322.0 42 276890.0 43 292458.0 44 292458.0 45 292458.0 46 302163.0 47 311868.0 48 311868.0 49 311868.0 50 301739.0 51 291610.0 52 291610.0 53 291610.0 54 261429.5 55 231249.0 56 231249.0 57 231249.0 58 198687.5 59 166126.0 60 166126.0 61 166126.0 62 137200.0 63 108274.0 64 108274.0 65 108274.0 66 83570.0 67 58866.0 68 58866.0 69 58866.0 70 43330.5 71 27795.0 72 27795.0 73 27795.0 74 19507.5 75 11220.0 76 11220.0 77 11220.0 78 7736.5 79 4253.0 80 4253.0 81 4253.0 82 2773.5 83 1294.0 84 1294.0 85 1294.0 86 814.0 87 334.0 88 334.0 89 334.0 90 209.0 91 84.0 92 84.0 93 84.0 94 49.5 95 15.0 96 15.0 97 15.0 98 8.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1534653279644087 2 0.015588276115885077 3 0.0 4 0.0036261497916631065 5 0.10107371545727624 6 0.0389706902897127 7 0.025841527250932486 8 0.025758167485606893 9 0.025924887016258072 10 0.02688352431750234 11 0.031051512583781773 12 0.03280206765561914 13 0.020089703443466868 14 0.010336610900372994 15 0.002959271669058397 16 0.002542472842430454 17 0.001417116010535007 18 0.0018755947198257445 19 0.0011670367145582411 20 4.167988266279433E-5 21 0.0 22 7.085580052675035E-4 23 0.0 24 1.25039647988383E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2399239.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.37706980391998 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34805513042538 57.13066769000573 2 11.785559912709626 16.352952254846738 3 3.0341052929150094 6.314920040970231 4 1.1196396830930628 3.107092817967451 5 0.5209263623251863 1.8070172300868281 6 0.3022663656316865 1.258221284868403 7 0.19575805504032134 0.9506784174448387 8 0.13316395259569422 0.7390819867677896 9 0.09697176827687906 0.6054855423079133 >10 0.4213741385453451 4.982687399823213 >50 0.022126792753957982 1.0594192187819973 >100 0.016505548657279243 2.2193333881754818 >500 0.0023446251558041373 1.1799339952425774 >1k 0.0010220160935529659 1.534790072706077 >5k 1.8035578121522927E-4 0.7577186600046552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7407 0.30872289088331756 No Hit TATCAACGCAGAGTACTTTTTTTTT 5423 0.22603000368033363 No Hit GGTATCAACGCAGAGTACTTTTTTT 5337 0.2224455337713333 No Hit GTCCTACAGTGGACATTTCTAAATT 3810 0.1588003529452464 No Hit CTGTAGGACGTGGAATATGGCAAGA 3672 0.15304852913778078 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3671 0.15300684925511798 No Hit CTTTAGGACGTGAAATATGGCGAGG 3621 0.15092285512197826 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2457 0.10240747170248567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.167988266279433E-5 0.0 0.0 0.0 0.0 6 4.167988266279433E-5 0.0 0.0 0.0 0.0 7 4.167988266279433E-5 0.0 0.0 0.0 0.0 8 4.167988266279433E-5 0.0 0.0 0.0 0.0 9 4.167988266279433E-5 0.0 0.0 0.0 0.0 10 4.167988266279433E-5 0.0 0.0 4.167988266279433E-5 0.0 11 4.167988266279433E-5 0.0 0.0 4.167988266279433E-5 0.0 12 4.167988266279433E-5 0.0 0.0 4.167988266279433E-5 0.0 13 4.167988266279433E-5 0.0 0.0 4.167988266279433E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1620 0.0 13.332227 1 TAGGACC 895 0.0 11.576285 4 GGCGAGG 1125 0.0 11.478876 19 TAGGTCG 70 0.0014844734 10.863652 5 AGGACCT 1595 0.0 10.429447 5 GTCCTAA 1220 0.0 10.216526 1 TGGCGAG 2445 0.0 10.136398 18 TTAGGAC 1570 0.0 10.04956 3 AGGACGT 2320 0.0 9.997369 5 GGACGTG 2265 0.0 9.940342 6 GGACCTG 1610 0.0 9.91299 6 TGCCGGT 155 4.0739906E-8 9.805171 10 GTAGGAC 2370 0.0 9.785434 3 CCTATAC 215 1.0913936E-11 9.725744 3 AATGTCC 685 0.0 9.706964 8 TGTAGGA 2500 0.0 9.658793 2 GACGTGA 1375 0.0 9.602582 7 GTCCTAC 2125 0.0 9.537015 1 TATGGCG 2600 0.0 9.532113 16 TGGACCG 110 6.794151E-5 9.505695 5 >>END_MODULE