FastQCFastQC Report
Thu 26 May 2016
SRR1512720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158703
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75220.3484499720433983No Hit
GTCCTACAGTGGACATTTCTAAATT73170.3389535290403543No Hit
GTCCTAAAGTGTGTATTTCTCATTT65990.3056928164735955No Hit
CTGTAGGACGTGGAATATGGCAAGA63910.2960574011339216No Hit
CTTTAGGACGTGAAATATGGCGAGG55570.2574230915508062No Hit
GGTATCAACGCAGAGTACTTTTTTT47960.22217044215901866No Hit
TATCAACGCAGAGTACTTTTTTTTT43460.2013245916645319No Hit
GTCCTACAGTGTGCATTTCTCATTT35700.16537708058959477No Hit
CTGAAGGACCTGGAATATGGCGAGA29900.13850909550781187No Hit
GTACATGGGAAGCAGTGGTATCAAC28350.13132885811526643No Hit
GTCCTTCAGTGTGCATTTCTCATTT25240.11692205921796561No Hit
ATTTAGAAATGTCCACTGTAGGACG25120.11636616987144596No Hit
CTGTAGGACCTGGAATATGGCGAGA24880.11525439117840666No Hit
GAATATGGCAAGAAAACTGAAAATC24550.11372569547547765No Hit
TTTCTAAATTTTCCACCTTTTTCAG23310.10798150556144129No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22180.10274688088171462No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21670.10038435115900612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11950.012.6227814
CCACCTT8400.011.85949613
TCCAACG2250.011.79085718
CACCTTT8750.011.71449714
AAATGTC9700.011.4430037
ACCTTTT8800.011.43223615
TTCCACC9400.011.1971811
TAGAAAT10500.011.0229134
GTGTAGG4550.011.0098991
CCAACGA1900.010.97311319
GGCGAGG17700.010.9223919
CTACACT3050.010.8866454
CCTACAC3350.010.7612983
GAAATGT10950.010.7431946
AATGTCC10300.010.6513198
GTTTAGG2900.010.4999751
AGGACCT24400.010.3811945
ATGTCCA11300.010.3807019
TTTAGAA11650.010.3419792
TCCACCT10150.010.18438112
Produced by FastQC (version 0.11.5)