FastQCFastQC Report
Thu 26 May 2016
SRR1512720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158703
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95890.44420191198140735No Hit
CTGTAGGACGTGGAATATGGCAAGA75310.348866889053288No Hit
GTCCTACAGTGGACATTTCTAAATT71220.32992032715941005No Hit
TATCAACGCAGAGTACTTTTTTTTT67530.31282672975393094No Hit
GGTATCAACGCAGAGTACTTTTTTT67190.3112517099387919No Hit
GTCCTAAAGTGTGTATTTCTCATTT67140.3110200893777421No Hit
CTTTAGGACGTGAAATATGGCGAGG66190.30661929871779486No Hit
CTGAAGGACCTGGAATATGGCGAGA37410.17329850377749972No Hit
GTCCTACAGTGTGCATTTCTCATTT35090.16255130974478657No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32800.15194308804870332No Hit
GTACATGGGAAGCAGTGGTATCAAC32090.14865407608179543No Hit
CTGTAGGACCTGGAATATGGCGAGA30140.13962087420085117No Hit
ATTTAGAAATGTCCACTGTAGGACG29900.13850909550781187No Hit
TTTCTAAATTTTCCACCTTTTTCAG28400.1315604786763163No Hit
GAATATGGCAAGAAAACTGAAAATC26730.12382435193725122No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24950.11557865996387647No Hit
GTCCTTCAGTGTGCATTTCTCATTT23600.10932490481553044No Hit
GGAATATGGCGAGAAAACTGAAAAT23440.1085837190201709No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22450.10399763191138382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11950.013.0454534
CCGAGCT550.003071353512.0897299
CGGGGTG550.003072419712.089169512
TCCAACG1604.5474735E-1111.27544718
AAATGTC9400.011.216997
GGCGAGG16600.011.21109819
TTTAGAA11450.011.2078312
ACCTTTT9750.011.19962315
GAAATGT9400.011.1167096
CACCTTT10450.010.904489514
TAGAAAT11000.010.8883254
CTACACG700.001484387610.8636354
AATGTCC9850.010.8009778
CCACCTT10200.010.80011413
AGGACCT25900.010.6436735
TGTCCAC10050.010.58603210
TGTAGGA41500.010.5137572
ATTTAGA11150.010.4958221
CTGTAGG41250.010.379461
CTAGTAC1106.004584E-610.3698343
Produced by FastQC (version 0.11.5)