##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512720_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158703 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493245712819224 33.0 31.0 34.0 28.0 34.0 2 31.64233152962682 34.0 31.0 34.0 28.0 34.0 3 31.740029545518766 34.0 31.0 34.0 28.0 34.0 4 35.20612284320724 37.0 35.0 37.0 32.0 37.0 5 34.9748631469915 37.0 35.0 37.0 32.0 37.0 6 34.83594084040278 37.0 35.0 37.0 32.0 37.0 7 34.8167909156563 37.0 35.0 37.0 32.0 37.0 8 34.80611552399751 37.0 35.0 37.0 32.0 37.0 9 36.38324493920655 39.0 37.0 39.0 32.0 39.0 10 36.17693772603271 39.0 37.0 39.0 32.0 39.0 11 36.28716687751859 39.0 37.0 39.0 32.0 39.0 12 36.22678108104728 39.0 37.0 39.0 32.0 39.0 13 36.223312794766116 39.0 37.0 39.0 32.0 39.0 14 37.433106360624876 40.0 38.0 41.0 32.0 41.0 15 37.424621636232494 40.0 38.0 41.0 32.0 41.0 16 37.38488759222552 40.0 38.0 41.0 32.0 41.0 17 37.37016208343621 40.0 38.0 41.0 32.0 41.0 18 37.30798493354575 40.0 38.0 41.0 32.0 41.0 19 37.33361699131377 40.0 38.0 41.0 32.0 41.0 20 37.29834071662475 40.0 38.0 41.0 32.0 41.0 21 37.24336094404835 40.0 38.0 41.0 32.0 41.0 22 37.14804398752399 40.0 38.0 41.0 31.0 41.0 23 37.084292281059504 40.0 38.0 41.0 31.0 41.0 24 37.04789496285501 40.0 38.0 41.0 31.0 41.0 25 36.97561313436818 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6493039900709228 1101 2 0.5119787403089227 1101 3 0.6012253670199961 1101 4 0.5872318825291245 1101 5 0.6446317173195197 1101 6 0.7197169650980157 1101 7 0.7807613254082995 1101 8 0.7575462722066106 1101 9 0.8492719647525035 1101 10 0.9717287711129146 1101 11 0.8786184196943694 1101 12 0.8389113877486167 1101 13 0.8647980793428403 1101 14 1.0324241194376071 1101 15 0.9383085535965776 1101 16 1.1619793281884156 1101 17 1.1020364211222642 1101 18 1.2048491802344543 1101 19 1.1000379816448387 1101 20 1.073902404138252 1101 21 1.102795134946092 1101 22 1.158987294131343 1101 23 1.1671917218450218 1101 24 1.2298296877440364 1101 25 1.084869394284837 1102 1 0.6412595818123812 1102 2 0.6335105805031951 1102 3 0.6044969228621149 1102 4 0.5579617762147393 1102 5 0.6663936935717345 1102 6 0.7573571527836194 1102 7 0.6635371239427741 1102 8 0.6193453337785968 1102 9 0.6544344954020147 1102 10 0.8495742928723047 1102 11 0.7972940313903649 1102 12 0.8525341246263949 1102 13 0.7707114935924508 1102 14 0.9166082659340304 1102 15 1.0023445000017048 1102 16 1.004556852037382 1102 17 1.0852230760546604 1102 18 1.1606569389727994 1102 19 1.0697489702886358 1102 20 1.0700308002922938 1102 21 1.1379174741449987 1102 22 1.106497918510705 1102 23 1.033400783275816 1102 24 1.0732912823186638 1102 25 1.0258870286367596 1103 1 0.4159708440319143 1103 2 0.4587613983525536 1103 3 0.42574322459764247 1103 4 0.4528098072705191 1103 5 0.5193397722866564 1103 6 0.6611799960071991 1103 7 0.4396679198542799 1103 8 0.5393816255092645 1103 9 0.6507526200578937 1103 10 0.7010449220646535 1103 11 0.6705789043371695 1103 12 0.6237663938450453 1103 13 0.8226999333693925 1103 14 0.7589575449197454 1103 15 0.8715629423709075 1103 16 0.8287049925682197 1103 17 0.7562297538188005 1103 18 0.8915713234809033 1103 19 0.7793367142241223 1103 20 0.759445997829431 1103 21 0.7917526359902638 1103 22 0.7110607359830041 1103 23 0.7655549567177218 1103 24 0.8383119668912471 1103 25 0.827484378954793 1104 1 0.41169952669114807 1104 2 0.4490321149524128 1104 3 0.47032289755231105 1104 4 0.4792108871208569 1104 5 0.47461176824216267 1104 6 0.535479904165534 1104 7 0.5416572869873235 1104 8 0.3887940489796975 1104 9 0.5118096521258408 1104 10 0.6685894615646077 1104 11 0.6203848996326045 1104 12 0.5598913182888978 1104 13 0.601558055918062 1104 14 0.7238556105587293 1104 15 0.684529180978771 1104 16 0.8027402533404526 1104 17 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-2.0508943131394872 2314 21 -1.9066246262530413 2314 22 -1.9177432186534205 2314 23 -1.856687979465157 2314 24 -1.978131714924082 2314 25 -2.1317895429519353 2315 1 -0.7275710365252017 2315 2 -0.575143062837224 2315 3 -0.7099900402907444 2315 4 -0.5154238288994009 2315 5 -0.5298078137376621 2315 6 -0.6034392144222593 2315 7 -0.6950051748257451 2315 8 -0.8272847183038934 2315 9 -1.0679378538264857 2315 10 -0.8801636316161989 2315 11 -0.9206748326921854 2315 12 -1.1646285560803733 2315 13 -1.1413305536874105 2315 14 -1.3491539880532812 2315 15 -1.3721107431310315 2315 16 -1.3595981055340474 2315 17 -1.355206766724372 2315 18 -1.230778452781614 2315 19 -1.2531762483442392 2315 20 -1.1739158273260273 2315 21 -0.9835651325754284 2315 22 -1.1725768060686619 2315 23 -1.170727078421649 2315 24 -1.0988070659687779 2315 25 -1.2660487038442128 2316 1 -1.480114349782177 2316 2 -1.3524143276477005 2316 3 -1.469519505248929 2316 4 -1.2916546127547974 2316 5 -1.3869482552062635 2316 6 -4.692317463221212 2316 7 -2.6710773119949494 2316 8 -2.06124638229344 2316 9 -2.022206354583311 2316 10 -1.7619227969889906 2316 11 -1.8831136225534237 2316 12 -1.9547406964669989 2316 13 -1.9178600759253683 2316 14 -2.139243429807067 2316 15 -2.218030595324848 2316 16 -2.1609148395146036 2316 17 -2.364222998836631 2316 18 -2.329072216355094 2316 19 -2.3843060979300006 2316 20 -2.5276703640058003 2316 21 -2.708060857310784 2316 22 -2.6091804385811486 2316 23 -2.6479579122445003 2316 24 -2.6177366593799007 2316 25 -2.5081114946609304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24623.0 3 2.0 4 469.0 5 4006.0 6 5008.0 7 3968.0 8 3149.0 9 2833.0 10 2546.0 11 2462.0 12 2254.0 13 2242.0 14 2203.0 15 2253.0 16 2330.0 17 2442.0 18 2652.0 19 3059.0 20 3804.0 21 5001.0 22 6262.0 23 7941.0 24 9702.0 25 11289.0 26 13048.0 27 14976.0 28 17237.0 29 20727.0 30 26921.0 31 34687.0 32 45137.0 33 59320.0 34 79162.0 35 108118.0 36 162345.0 37 302527.0 38 1161998.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.480196878321536 20.186940689222588 13.500472988615334 24.832389443840547 2 14.394333204534105 21.173342451206867 41.98609338963435 22.44623095462468 3 18.704703704029686 25.905184733610874 30.653776828030534 24.7363347343289 4 12.434646290855623 17.959750546409357 38.777321625951174 30.82828153678384 5 12.224642180317842 38.91722305867144 36.21111535131872 12.647019409692001 6 30.572246304478192 38.11454935904743 18.39118172356042 12.922022612913958 7 26.947281701130766 32.07547257236163 23.08260448116661 17.894641245341 8 24.146189912884893 38.68182388949202 20.905170516440016 16.266815681183072 9 26.360046668563307 14.750685525609791 20.97847370815838 37.910794097668514 10 15.339192306819061 29.23021391724752 34.97183494727712 20.4587588286563 11 33.240811492232105 22.021928139722498 26.304268346084818 18.432992021960576 12 23.797875308032143 24.785987746367795 32.70793965303926 18.708197292560797 13 29.378337368097945 22.567592925069206 26.06910451391704 21.984965192915805 14 19.90495374625671 23.794965828564653 28.212050063747146 28.088030361431493 15 22.064738129937655 33.33165019507418 23.54454093407688 21.059070740911285 16 20.383337888607336 28.359545460862474 30.254230614220795 21.002886036309395 17 20.579193862944656 29.803121337477823 28.97873247200849 20.638952327569037 18 20.45506449997522 27.038198753591892 32.595244415229104 19.911492331203796 19 23.140088136745835 26.741576260798787 28.799629034447268 21.31870656800811 20 23.436826128305867 27.621287061061256 29.433256388911666 19.50863042172121 21 21.79637495292312 26.816704289566466 28.379494539082035 23.00742621842838 22 22.741513259655967 29.21562187455268 28.178928804539417 19.863936061251934 23 20.7118811619755 28.96456807629396 29.46750896255761 20.856041799172928 24 22.75690541959686 27.12670524847559 30.02168431692549 20.094705015002067 25 21.589398819568974 28.107247731624035 29.631913236790798 20.671440212016197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 408.0 1 408.0 2 665.0 3 922.0 4 922.0 5 922.0 6 2251.5 7 3581.0 8 3581.0 9 3581.0 10 4730.0 11 5879.0 12 5879.0 13 5879.0 14 8483.5 15 11088.0 16 11088.0 17 11088.0 18 20014.5 19 28941.0 20 28941.0 21 28941.0 22 46686.0 23 64431.0 24 64431.0 25 64431.0 26 94839.5 27 125248.0 28 125248.0 29 125248.0 30 161627.5 31 198007.0 32 198007.0 33 198007.0 34 217221.5 35 236436.0 36 236436.0 37 236436.0 38 243992.0 39 251548.0 40 251548.0 41 251548.0 42 258896.5 43 266245.0 44 266245.0 45 266245.0 46 276209.0 47 286173.0 48 286173.0 49 286173.0 50 266096.5 51 246020.0 52 246020.0 53 246020.0 54 210641.0 55 175262.0 56 175262.0 57 175262.0 58 147122.0 59 118982.0 60 118982.0 61 118982.0 62 96943.0 63 74904.0 64 74904.0 65 74904.0 66 56749.5 67 38595.0 68 38595.0 69 38595.0 70 27673.0 71 16751.0 72 16751.0 73 16751.0 74 11537.5 75 6324.0 76 6324.0 77 6324.0 78 4230.5 79 2137.0 80 2137.0 81 2137.0 82 1370.0 83 603.0 84 603.0 85 603.0 86 391.5 87 180.0 88 180.0 89 180.0 90 103.0 91 26.0 92 26.0 93 26.0 94 18.5 95 11.0 96 11.0 97 11.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1510166058045039 2 0.01412885422404101 3 0.0 4 0.003011067293648084 5 0.0972806356409381 6 0.0380320961243858 7 0.02385691778813482 8 0.024273834798024554 9 0.023393676666035115 10 0.02464442769570432 11 0.03029596938532072 12 0.03006434882427087 13 0.01751051441536886 14 0.008106719636744841 15 0.0017139921517689094 16 0.0015286957029290273 17 6.022134587296168E-4 18 9.264822441994104E-4 19 5.558893465196463E-4 20 9.264822441994106E-5 21 0.0 22 5.558893465196463E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2158703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.93682100712058 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68594731175443 49.354942554309766 2 13.484443612114857 16.70367140768337 3 3.6013725374052377 6.691726986876836 4 1.3142860112182495 3.256107897159488 5 0.6090766600573604 1.8862136036793782 6 0.32697963963246707 1.215124764773334 7 0.1957871098094385 0.8488501823038058 8 0.13515690758710128 0.6696951354478584 9 0.09394844384643074 0.523698115537251 >10 0.4747089251281873 5.23086143164636 >50 0.03895617510358453 1.6612772505839652 >100 0.031247434779184964 4.1082652125459775 >500 0.0042693166585279305 1.8091507562408904 >1k 0.003295612859214463 3.672300473460851 >5k 5.243020457841192E-4 2.3681142277508345 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9589 0.44420191198140735 No Hit CTGTAGGACGTGGAATATGGCAAGA 7531 0.348866889053288 No Hit GTCCTACAGTGGACATTTCTAAATT 7122 0.32992032715941005 No Hit TATCAACGCAGAGTACTTTTTTTTT 6753 0.31282672975393094 No Hit GGTATCAACGCAGAGTACTTTTTTT 6719 0.3112517099387919 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6714 0.3110200893777421 No Hit CTTTAGGACGTGAAATATGGCGAGG 6619 0.30661929871779486 No Hit CTGAAGGACCTGGAATATGGCGAGA 3741 0.17329850377749972 No Hit GTCCTACAGTGTGCATTTCTCATTT 3509 0.16255130974478657 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3280 0.15194308804870332 No Hit GTACATGGGAAGCAGTGGTATCAAC 3209 0.14865407608179543 No Hit CTGTAGGACCTGGAATATGGCGAGA 3014 0.13962087420085117 No Hit ATTTAGAAATGTCCACTGTAGGACG 2990 0.13850909550781187 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2840 0.1315604786763163 No Hit GAATATGGCAAGAAAACTGAAAATC 2673 0.12382435193725122 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2495 0.11557865996387647 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2360 0.10932490481553044 No Hit GGAATATGGCGAGAAAACTGAAAAT 2344 0.1085837190201709 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2245 0.10399763191138382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.632411220997052E-5 8 0.0 0.0 0.0 0.0 4.632411220997052E-5 9 0.0 0.0 0.0 0.0 9.264822441994104E-5 10 0.0 0.0 0.0 0.0 9.264822441994104E-5 11 0.0 0.0 0.0 0.0 9.264822441994104E-5 12 0.0 0.0 0.0 4.632411220997052E-5 9.264822441994104E-5 13 0.0 0.0 0.0 9.264822441994104E-5 9.264822441994104E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1195 0.0 13.045453 4 CCGAGCT 55 0.0030713535 12.089729 9 CGGGGTG 55 0.0030724197 12.0891695 12 TCCAACG 160 4.5474735E-11 11.275447 18 AAATGTC 940 0.0 11.21699 7 GGCGAGG 1660 0.0 11.211098 19 TTTAGAA 1145 0.0 11.207831 2 ACCTTTT 975 0.0 11.199623 15 GAAATGT 940 0.0 11.116709 6 CACCTTT 1045 0.0 10.9044895 14 TAGAAAT 1100 0.0 10.888325 4 CTACACG 70 0.0014843876 10.863635 4 AATGTCC 985 0.0 10.800977 8 CCACCTT 1020 0.0 10.800114 13 AGGACCT 2590 0.0 10.643673 5 TGTCCAC 1005 0.0 10.586032 10 TGTAGGA 4150 0.0 10.513757 2 ATTTAGA 1115 0.0 10.495822 1 CTGTAGG 4125 0.0 10.37946 1 CTAGTAC 110 6.004584E-6 10.369834 3 >>END_MODULE