FastQCFastQC Report
Thu 26 May 2016
SRR1512719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399594
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67050.279422268933828No Hit
GGTATCAACGCAGAGTACTTTTTTT44080.18369774220138907No Hit
TATCAACGCAGAGTACTTTTTTTTT41170.17157069070851153No Hit
GTCCTACAGTGGACATTTCTAAATT24490.10205893163593507No Hit
GTCCTAAAGTGTGTATTTCTCATTT24010.10005859324535733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.011.3797891
GTCTTAT1603.074092E-1011.042711
TAGGACC5200.010.9472844
ACCGTCT700.001547112510.809958
GTATAAT3100.010.7656531
GTGTAGG3600.010.633721
GTCCTAG2600.010.1932711
GGACGTG16400.010.0661616
CCTTATA1804.0381565E-1010.01431752
GTATTAT2850.09.9879481
AGGACGT16750.09.9691095
CCGGTTT1054.187955E-59.9346512
ATAGGCC1451.6499507E-79.8143963
TGTAGGA17100.09.7091722
GTAGGAC15650.09.6994243
AGGACCT11550.09.69300655
GTTCTAA2550.09.6232781
GACGTGG8050.09.5465757
TAGGACG17600.09.54155354
GACGTGA9350.09.5383837
Produced by FastQC (version 0.11.5)