FastQCFastQC Report
Thu 26 May 2016
SRR1512719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399594
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82700.34464163520995633No Hit
GGTATCAACGCAGAGTACTTTTTTT56250.23441465514582885No Hit
TATCAACGCAGAGTACTTTTTTTTT54200.2258715432694031No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26750.1114771915582386No Hit
CTGTAGGACGTGGAATATGGCAAGA25760.10735149362767202No Hit
GTCCTACAGTGGACATTTCTAAATT25580.10660136673120536No Hit
CTTTAGGACGTGAAATATGGCGAGG25330.10555952381944612No Hit
GTCCTAAAGTGTGTATTTCTCATTT24490.10205893163593507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.011.633311
TAGACAG3350.011.0663675
TAGGCCC1303.239802E-810.9681454
TAGGACC4700.010.92147354
GCATAAC1053.398738E-610.8754831
CGCCCTA909.56367E-510.550693516
AGGACGT15550.010.4532425
TAGGACA5650.010.4310634
GGACGTG15600.010.3523256
TGTAGGA16100.010.274772
TAGGACG16650.010.1051864
GTAGGAC14950.09.9823933
TAAGACT2002.5465852E-119.9810124
TTAGGAC9850.09.8432873
TGGCGAG15100.09.80984418
GACGTGA8350.09.7829837
GGCGAGG8200.09.72702219
CTGTAGG16450.09.7185171
GTATCAA39500.09.6846871
ACCGTCC1106.849941E-59.4985928
Produced by FastQC (version 0.11.5)