FastQCFastQC Report
Thu 26 May 2016
SRR1512718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865066
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84730.29573489755558857No Hit
GTCCTAAAGTGTGTATTTCTCATTT73460.2563989799885936No Hit
CTGTAGGACGTGGAATATGGCAAGA71860.25081446640321725No Hit
GTATCAACGCAGAGTACTTTTTTTT70410.2457535009664699No Hit
CTTTAGGACGTGAAATATGGCGAGG61410.21434061204872767No Hit
GTCCTACAGTGTGCATTTCTCATTT47270.1649874732379638No Hit
GGTATCAACGCAGAGTACTTTTTTT42220.14736135223411956No Hit
TATCAACGCAGAGTACTTTTTTTTT40480.14128819371002274No Hit
CTGAAGGACCTGGAATATGGCGAGA32700.11413349640113002No Hit
CTGTAGGACCTGGAATATGGCGAGA31080.10847917639593642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2450.012.7648718
TAGGACC15600.011.9190574
TAGAAAT10600.011.5450114
AGGACCG1751.8189894E-1211.3839175
GGATCGT855.4033706E-511.1607046
CCAACGA2000.010.90135919
GGCGAGG19400.010.89647219
GTATTAA3250.010.8773261
GTACCGA700.001509737810.84182556
TGTAGGA48550.010.8250772
GACGTGG24550.010.7811027
CACCTTT10400.010.67692114
TCGAACT2050.010.63547119
GTCTACG650.00832983310.5751791
TAGGACT4850.010.5623974
CGGACTG909.65777E-510.5406645
GTCCTAC41850.010.5120051
GTAGGAC48400.010.5058183
AGGACGT47300.010.4693535
ACCTTTT11000.010.43965515
Produced by FastQC (version 0.11.5)