FastQCFastQC Report
Thu 26 May 2016
SRR1512718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865066
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90490.3158391464629436No Hit
CTGTAGGACGTGGAATATGGCAAGA87460.30526347386063707No Hit
GTCCTACAGTGGACATTTCTAAATT87350.3048795385516424No Hit
GTCCTAAAGTGTGTATTTCTCATTT75860.26477575036665824No Hit
CTTTAGGACGTGAAATATGGCGAGG75300.2628211706117765No Hit
TATCAACGCAGAGTACTTTTTTTTT60730.21196719377494272No Hit
GGTATCAACGCAGAGTACTTTTTTT59250.20680151870846952No Hit
GTCCTACAGTGTGCATTTCTCATTT50970.1779016609041467No Hit
CTGAAGGACCTGGAATATGGCGAGA39200.13682058284172163No Hit
CTGTAGGACCTGGAATATGGCGAGA39130.1365762603723614No Hit
TTTCTAAATTTTCCACCTTTTTCAG29270.10216169540247938No Hit
GTACATGGGAAGCAGTGGTATCAAC29180.10184756651330197No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29020.10128911515476433No Hit
ATTTAGAAATGTCCACTGTAGGACG28900.1008702766358611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT400.005286660514.2475128
GTCCTAG4100.012.3023991
TAGGACC15150.012.23434454
TAGAACG1103.7896825E-812.0974574
GGCGAGG18600.011.33356519
CACCTTT10050.011.14994614
TATGTCG2100.010.85221716
TGTAGGA45850.010.7405142
AGGACCT29400.010.7342645
CTGTAGG46100.010.5285121
GGACCGT1002.4035508E-510.4487236
TGGCGAG45600.010.37029318
GGACCTG28500.010.332076
ACCTTTT11150.010.30473915
TTAGGAC28700.010.2668813
GTAGGAC46450.010.2520513
AGGACGT46900.010.2352875
CCACCTT11100.010.09610313
GGACGTG46500.010.091246
GACGTGG23250.010.0907127
Produced by FastQC (version 0.11.5)