FastQCFastQC Report
Thu 26 May 2016
SRR1512717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176951
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51152.8906307395832744No Hit
GGTATCAACGCAGAGTACTTTTTTT39882.2537312589360896No Hit
TATCAACGCAGAGTACTTTTTTTTT30741.7372040847466248No Hit
GTACATGGGAAGCAGTGGTATCAAC26951.5230204971997898No Hit
GTACTGGTTCACTATCGGTCAGTCA17961.0149702460003052No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17630.9963210154223485No Hit
CCCATGTACTCTGCGTTGATACCAC16340.9234194777085182No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16230.9172030675158659No Hit
GAGTACATGGGAAGCAGTGGTATCA12530.7081056337630192No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11770.6651558906137858No Hit
ATACAGGGTGACAGCCCCGTACACA10920.6171199936705641No Hit
GGATACCACGTGTCCCGCCCTACTC10310.5826471735113111No Hit
GTACATGGGGGTTAAGCGACTAAGC8730.4933569180168521No Hit
GTGGTATCAACGCAGAGTACATGGG8520.4814892258308797No Hit
GGTATCAACGCAGAGTACATGGGGG8310.46962153364490733No Hit
ATCATTAACTGAATCCATAGGTTAA7890.4458861492729626No Hit
ACGCAGAGTACATGGGAAGCAGTGG7540.426106662296342No Hit
GGTTAATGAGGCGAACCGGGGGAAC7490.4232810212996818No Hit
GGTATCAACGCAGAGTACATGGGAA7420.41932512390435767No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7200.4068923035190533No Hit
GTATTTAGCCTTGGAGGATGGTCCC7050.39841538052907305No Hit
CATGTACTCTGCGTTGATACCACTG6990.3950246113330809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6540.3695938423631401No Hit
GTATCAACGCAGAGTACATGGGAAG6530.36902871416380806No Hit
TATCAACGCAGAGTACATGGGAAGC6520.36846358596447604No Hit
GTGGTATCAACGCAGAGTACTTTTT6500.367333329565812No Hit
GTATCAACGCAGAGTACATGGGGGT6490.36676820136648No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6240.3526399963831795No Hit
GCGTTGATACCACTGCTTCCCATGT6230.35207486818384753No Hit
GTTCACTATCGGTCAGTCAGGAGTA5850.33059999660923084No Hit
GCCTTGGAGGATGGTCCCCCCATAT5740.3243835864165786No Hit
GCCCAGAGCCTGAATCAGTGTGTGT5510.3113856378319422No Hit
CCCATATTCAGACAGGATACCACGT5500.31082050963261015No Hit
AGCGTACACGGTGGATGCCCTGGCA5260.2972574328486417No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5230.29556204825064564No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5220.2949969200513136No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5080.2870851252606654No Hit
ACTCTGCGTTGATACCACTGCTTCC4970.2808687150680132No Hit
ACGCAGAGTACATGGGGGTTAAGCG4970.2808687150680132No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4960.2803035868686811No Hit
ATCAACGCAGAGTACTTTTTTTTTT4710.2661753818853807No Hit
CTATCGGTCAGTCAGGAGTATTTAG4700.2656102536860487No Hit
ACCCTGTATCGCGCGCCTTTCCAGA4690.26504512548671666No Hit
TATTCAGACAGGATACCACGTGTCC4690.26504512548671666No Hit
CGGTACTGGTTCACTATCGGTCAGT4660.2633497408887206No Hit
CATCTAAGTACCCCGAGGAAAAGAA4530.2560030742974044No Hit
GGTATCAACGCAGAGTACATGGGGA4430.2503517923040842No Hit
GGTTCACTATCGGTCAGTCAGGAGT4190.23678871552011574No Hit
GTTAATGATAGTGTGTCGAAACACA4190.23678871552011574No Hit
TCACGGTACTGGTTCACTATCGGTC3990.22548615153347534No Hit
GTACTTAGATGTTTCAGTTCCCCCG3920.22153025413815122No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT3850.2175743567428271No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3800.214748715746167No Hit
GTGCTAATCTGCGATAAGCGTCGGT3790.214183587546835No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3770.213053331148171No Hit
GTTATAACCGGCGATTTCCGAATGG3690.20853230555351482No Hit
GATATGAACCGTTATAACCGGCGAT3670.20740204915485078No Hit
GCTTCCCATGTACTCTGCGTTGATA3400.1921435877728863No Hit
TATCAACGCAGAGTACATGGGGGTT3350.18931794677622618No Hit
GGGAAGCAGTGGTATCAACGCAGAG3340.1887528185768942No Hit
GAGTACATGGGGGTTAAGCGACTAA3240.18310153658357398No Hit
GTACTCTGCGTTGATACCACTGCTT3220.18197128018490996No Hit
CACTATCATTAACTGAATCCATAGG3200.1808410237862459No Hit
GCCCTACTCATCGAGCTCACAGCAT3160.17858051098891783No Hit
GGTGATATGAACCGTTATAACCGGC3080.1740594853942617No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3080.1740594853942617No Hit
GGAGTATTTAGCCTTGGAGGATGGT3070.17349435719492967No Hit
GTCCCGCCCTACTCATCGAGCTCAC3050.17236410079626563No Hit
TCATTAACCTATGGATTCAGTTAAT3020.1706687161982696No Hit
GGGTGACAGCCCCGTACACAAAAAT2990.16897333160027353No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2950.16671281880294544No Hit
CAGTGGTATCAACGCAGAGTACATG2880.16275692140762132No Hit
GGGTACTTAGATGTTTCAGTTCCCC2850.16106153680962526No Hit
ACATGGGAAGCAGTGGTATCAACGC2830.15993128041096122No Hit
GCAGTGGTATCAACGCAGAGTACAT2820.15936615221162923No Hit
TCGGTAAGGTGATATGAACCGTTAT2750.15541025481630508No Hit
ATCCATAGGTTAATGAGGCGAACCG2740.15484512661697306No Hit
GCAGAGTACATGGGAAGCAGTGGTA2690.15201948562031298No Hit
GTGGTATCCTGTCTGAATATGGGGG2680.15145435742098093No Hit
ATACCACTGCTTCCCATGTACTCTG2640.14919384462365287No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2620.14806358822498886No Hit
CTATCATTAACTGAATCCATAGGTT2610.1474984600256568No Hit
CATGGGAAGCAGTGGTATCAACGCA2560.14467281902899673No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2560.14467281902899673No Hit
GGGAAACCCAGTGTGTTTCGACACA2510.14184717803233665No Hit
AGCTCACAGCATGTGCATTTTTGTG2480.14015179343434056No Hit
GTCCCCCCATATTCAGACAGGATAC2460.13902153703567655No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2330.1316748704443603No Hit
GGGTTTCCCCATTCGGAAATCGCCG2300.12997948584636426No Hit
CACACACACTGATTCAGGCTCTGGG2300.12997948584636426No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2260.12771897304903618No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2230.1260235884510401No Hit
GATCAACGCAGAGTACTTTTTTTTT2220.1254584602517081No Hit
GATACCACTGCTTCCCATGTACTCT2210.12489333205237607No Hit
ATATCAACGCAGAGTACTTTTTTTT2200.12432820385304406No Hit
ATGGGAAGCAGTGGTATCAACGCAG2180.12319794745438004No Hit
CTGCTTCCCATGTACTCTGCGTTGA2140.12093743465705195No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2120.11980717825838792No Hit
CCGTTATAACCGGCGATTTCCGAAT2100.11867692185972387No Hit
GCGTACACGGTGGATGCCCTGGCAG2100.11867692185972387No Hit
NTATCAACGCAGAGTACTTTTTTTT2090.11811179366039186No Hit
TAAGTACCCCGAGGAAAAGAAATCA2080.11754666546105984No Hit
GAGTAGGGCGGGACACGTGGTATCC2070.11698153726172782No Hit
GTACATGGGCATCTAAGTACCCCGA2060.11641640906239581No Hit
GTGTTTCGACACACTATCATTAACT2050.11585128086306379No Hit
GGAGGATGGTCCCCCCATATTCAGA2050.11585128086306379No Hit
GGTATCAACGCAGAGTACATGGGCA2040.11528615266373178No Hit
TTTGTGTACGGGGCTGTCACCCTGT2020.11415589626506772No Hit
GGGGAAACCCAGTGTGTTTCGACAC2010.1135907680657357No Hit
ACCATGTACTCTGCGTTGATACCAC2000.1130256398664037No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1980.11189538346773967No Hit
CCTATGGATTCAGTTAATGATAGTG1980.11189538346773967No Hit
AAGCAGTGGTATCAACGCAGAGTAC1970.11133025526840763No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1940.10963487067041158No Hit
ATGATAGTGTGTCGAAACACACTGG1940.10963487067041158No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1900.1073743578730835No Hit
GTTGATACCACTGCTTCCCATGTAC1900.1073743578730835No Hit
GTATCAACGCAGAGTACATGGGGAG1860.10511384507575544No Hit
TTCCAGACGCTTCCACTAACACACA1850.10454871687642342No Hit
GAATATGGGGGGACCATCCTCCAAG1830.10341846047775938No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1830.10341846047775938No Hit
AACGCAGAGTACTTTTTTTTTTTTT1780.1005928194810993No Hit
AAGTACCCCGAGGAAAAGAAATCAA1780.1005928194810993No Hit
CAACGCAGAGTACTTTTTTTTTTTT1780.1005928194810993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA451.1921766E-619.6564711
AAGTACA250.00493911219.656471
CCGCAGA250.00493911219.656471
ATACATG350.001720827916.8484041
ACCATGT601.84455E-514.7423531
GGGTATC500.001153181813.7595291
AGGGTGA2150.012.3561945
ATCGCCG550.00310574112.06167219
AATCGCC550.00310574112.06167218
GGAGCGA550.00313224612.0480049
GGGTGAC2300.011.5503546
ACAGGGT2350.011.3046023
CCCCCGG855.3922085E-511.14944518
GGAAATC600.00590933511.06594815
GGTGACA2450.010.8431897
GTGACAG2450.010.8186168
GATCAAC650.00822344510.5842541
AGCCCCG2550.010.415009513
CCGTACA2550.010.41205417
GACAGCC2550.010.409110
Produced by FastQC (version 0.11.5)