##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512717_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176951 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.680442608405716 31.0 23.0 33.0 10.0 34.0 2 27.430949810964616 31.0 25.0 33.0 16.0 34.0 3 28.655379172765343 31.0 28.0 33.0 16.0 34.0 4 32.65788834197038 35.0 33.0 37.0 26.0 37.0 5 33.095857045170696 35.0 35.0 37.0 28.0 37.0 6 33.46042689784178 37.0 35.0 37.0 28.0 37.0 7 33.402286508694495 37.0 35.0 37.0 28.0 37.0 8 33.46915247723946 37.0 35.0 37.0 28.0 37.0 9 34.83112839147561 39.0 35.0 39.0 27.0 39.0 10 34.780475950969475 39.0 35.0 39.0 27.0 39.0 11 34.812004453210214 39.0 35.0 39.0 27.0 39.0 12 34.699436567185266 39.0 35.0 39.0 27.0 39.0 13 34.6732654802742 39.0 35.0 39.0 27.0 39.0 14 35.4759735746054 40.0 36.0 41.0 27.0 41.0 15 35.29579940209437 39.0 35.0 41.0 26.0 41.0 16 35.00014693333183 39.0 35.0 41.0 25.0 41.0 17 35.2226774643828 39.0 36.0 41.0 25.0 41.0 18 35.242473905205394 39.0 36.0 41.0 25.0 41.0 19 35.20776373120243 39.0 35.0 41.0 25.0 41.0 20 35.267373453667965 39.0 36.0 41.0 25.0 41.0 21 35.151906459980445 40.0 35.0 41.0 25.0 41.0 22 35.026538420240634 39.0 35.0 41.0 24.0 41.0 23 34.796847714904125 39.0 35.0 41.0 22.0 41.0 24 34.574057224881464 39.0 35.0 41.0 19.0 41.0 25 34.38378986273036 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.437235021925808 1101 2 1.0107639982342356 1101 3 0.19800790441537686 1101 4 -0.3491004237378519 1101 5 -0.7351835184242148 1101 6 -0.8862389260148973 1101 7 -0.9966886566311928 1101 8 -0.9926302114599039 1101 9 -1.2340546057454915 1101 10 -1.5324846250804427 1101 11 -1.4252583858682186 1101 12 -0.9166951797987579 1101 13 -1.078107799328997 1101 14 -2.0550414734649394 1101 15 -2.4745756246108 1101 16 -2.235172723658131 1101 17 -1.5555166034412764 1101 18 -1.5388644385364643 1101 19 -1.6806985921808888 1101 20 -1.5950612920472977 1101 21 -1.3127860951483896 1101 22 -1.7052429695037503 1101 23 -1.91280390575659 1101 24 -2.5760768499238935 1101 25 -2.652841707350902 1102 1 3.8931607053437176 1102 2 2.1292776665923974 1102 3 0.8046538366465121 1102 4 0.2115478001940474 1102 5 0.2864239459725795 1102 6 -0.7312217382591228 1102 7 -0.3988821416532886 1102 8 -0.21742150299698437 1102 9 -0.3577246008347075 1102 10 -1.0083808437741766 1102 11 -0.18233810431641473 1102 12 -0.9112114672435112 1102 13 -0.6847128083321081 1102 14 -0.8572595124238518 1102 15 -1.819722129767129 1102 16 -0.9809167079436136 1102 17 -0.9152317777742311 1102 18 -0.814441456051604 1102 19 -0.7641001308942634 1102 20 -0.6834145413516026 1102 21 -0.7158798911870221 1102 22 -0.6458633656405937 1102 23 -0.8562070813990843 1102 24 -0.8146592693051318 1102 25 -0.7156588623680875 1103 1 3.576087534612011 1103 2 1.4951313251289804 1103 3 0.5790440805489503 1103 4 -0.1238180534644826 1103 5 -0.036746785734735 1103 6 -0.2617095431371723 1103 7 -0.2647358001898752 1103 8 -0.17473857616771227 1103 9 0.09349491136041621 1103 10 -0.41691742914002816 1103 11 -0.2189234701700684 1103 12 -0.27096756480448647 1103 13 -0.39812744247844734 1103 14 -0.4060400002287281 1103 15 -0.7526489590354188 1103 16 -1.011404512821663 1103 17 -0.6713293387498354 1103 18 -0.5522463341003885 1103 19 -0.5750757406503624 1103 20 -0.3724389315955037 1103 21 -0.5939286716748313 1103 22 -0.27391214612840287 1103 23 -0.350109520423473 1103 24 -0.6805129278417184 1103 25 -0.07541495992906988 1104 1 3.0373434049346884 1104 2 1.348335385119892 1104 3 -0.13247690112049426 1104 4 -0.7804231133039039 1104 5 -0.6778238952636002 1104 6 -0.9561876525145436 1104 7 -1.1219544034318076 1104 8 -1.2217769036938435 1104 9 -1.7547099060914846 1104 10 -1.8780596803140952 1104 11 -1.799971796181282 1104 12 -1.879928328325171 1104 13 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1107 11 0.2356796596762578 1107 12 -0.1835221961311504 1107 13 0.33823234886512665 1107 14 0.6079526685996939 1107 15 0.2712830866434146 1107 16 0.438334666651059 1107 17 0.0376090512120939 1107 18 0.13332394645791368 1107 19 0.2575409236665678 1107 20 -0.11866760775509988 1107 21 -0.5022185349202815 1107 22 0.06459060306094244 1107 23 0.39153717350011874 1107 24 0.2817033417198189 1107 25 0.08424949798718728 1108 1 3.3769467363858467 1108 2 1.9584073783440594 1108 3 0.5121094907485073 1108 4 0.41512318822066163 1108 5 0.2806035468594956 1108 6 0.28513081163001175 1108 7 -0.5996859110546211 1108 8 -0.7753483322652741 1108 9 -0.4221647338723997 1108 10 -0.5250105555258386 1108 11 -0.8655420954472319 1108 12 -0.5370429528311007 1108 13 -0.8250952917023966 1108 14 -0.6073980933551155 1108 15 -0.20234408098664147 1108 16 -0.6018978609369583 1108 17 -0.49408432766336574 1108 18 -0.07040043609595159 1108 19 -0.4364948093865024 1108 20 -0.4489356056531548 1108 21 -0.08547523486772945 1108 22 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15 -0.6426979622651814 2314 16 -0.9265398285583828 2314 17 -0.547763240075156 2314 18 -0.39384896245543644 2314 19 -0.4363910318854636 2314 20 -0.5035225711990279 2314 21 -0.555561370193594 2314 22 -0.8249757389561125 2314 23 -0.8794000873017396 2314 24 -0.9882309365286588 2314 25 -0.312245341931515 2315 1 3.5589432712411266 2315 2 1.9337182222087144 2315 3 0.8506999921753327 2315 4 1.1229554344497643 2315 5 0.7100267021795119 2315 6 0.6289445091432597 2315 7 0.5763994945711417 2315 8 0.4079476318602886 2315 9 0.7632969457036296 2315 10 0.9035463136139938 2315 11 0.9684876640309596 2315 12 0.596772783002578 2315 13 0.8161348346734698 2315 14 0.02934772808801256 2315 15 0.3461424660985628 2315 16 -0.03109170520927762 2315 17 0.3770683425363899 2315 18 0.16505105187795976 2315 19 0.19997074299833173 2315 20 -0.09528701209424639 2315 21 0.28154983647348075 2315 22 0.3275315813133588 2315 23 0.23334230064182293 2315 24 0.38642352627618237 2315 25 0.39428863845220974 2316 1 3.7476635196026287 2316 2 1.511641644078324 2316 3 0.49733676347577926 2316 4 0.19090990150737497 2316 5 -0.6835572923013444 2316 6 -0.5465125450133499 2316 7 -0.8286194774881821 2316 8 -0.7234252553421996 2316 9 -1.2028465520542184 2316 10 -1.1522832827985638 2316 11 -0.8552274101325494 2316 12 -0.2041758199639645 2316 13 -0.3949379490450511 2316 14 -0.731086904543929 2316 15 -0.7686902348327962 2316 16 -0.6283838749229744 2316 17 -0.773017894096931 2316 18 -0.3236347018302155 2316 19 -0.324606697498389 2316 20 -0.6520824588000096 2316 21 0.0787730168805183 2316 22 -0.28038494162558436 2316 23 -0.1747811902170895 2316 24 -0.8512446351587926 2316 25 -1.230668243231129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7335.0 3 1.0 4 65.0 5 244.0 6 326.0 7 353.0 8 284.0 9 283.0 10 271.0 11 297.0 12 289.0 13 317.0 14 308.0 15 309.0 16 310.0 17 319.0 18 360.0 19 450.0 20 546.0 21 723.0 22 835.0 23 1043.0 24 1187.0 25 1389.0 26 1513.0 27 1742.0 28 2025.0 29 2516.0 30 3174.0 31 4012.0 32 5249.0 33 7271.0 34 10270.0 35 15776.0 36 24936.0 37 33287.0 38 47336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.119042028645225 19.835642169523364 11.172223526649448 16.873092275181968 2 18.49377879923466 22.04365596023866 36.782071167366716 22.680494073159963 3 23.42117476596371 30.331329733645756 26.61335053938715 19.634144961003386 4 13.897691263014938 18.728497057492078 32.3590991398823 35.014712539610684 5 13.214037191882733 39.427129594532666 30.043166118839775 17.31566709474482 6 29.104912926518477 32.64165368823446 19.311907121619708 18.941526263627352 7 25.74664767536219 35.46739605381878 19.39496427781025 19.39099199300878 8 30.08918756587074 28.30373635838216 21.170997608821295 20.436078466925807 9 30.965154287591844 12.583205397658068 19.792545929379507 36.65909438537058 10 23.022980497448184 27.319432879614368 24.75798822936315 24.899598393574294 11 32.67786550071928 25.867947396440993 17.8998221628173 23.554364940022428 12 26.64442380024471 29.011646349753022 25.699007567861514 18.64492228214075 13 33.95927153255728 21.09949868298071 24.89194777239641 20.049282012065596 14 25.34821150414908 27.462120139341245 26.330963776940724 20.858704579568947 15 31.94418467572362 23.836566745447435 26.009555710472178 18.20969286835677 16 20.68002180430635 27.99298173889343 27.518283819387662 23.808712637412555 17 23.034990289939014 27.678784368505333 25.377897402700643 23.90832793885501 18 22.37980878154878 21.185259866781458 32.36723458244597 24.067696769223797 19 22.283811864090094 26.157164938822426 33.91745981417417 17.641563382913308 20 25.013587879611386 20.040423941843873 33.7726747741015 21.173313404443235 21 25.299001064625003 23.395134437221103 33.42979137880263 17.876073119351258 22 25.093225429857075 24.04819386355651 29.959082820451787 20.89949788613463 23 23.546981178057504 23.959579014185884 32.832132756754305 19.661307051002307 24 23.68383757645845 25.22746211799119 30.66501136601985 20.423688939530507 25 22.780940930950006 24.049541783867163 31.75074857091008 21.41876871427275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1257.0 1 1257.0 2 1059.5 3 862.0 4 862.0 5 862.0 6 1444.0 7 2026.0 8 2026.0 9 2026.0 10 1760.0 11 1494.0 12 1494.0 13 1494.0 14 1014.0 15 534.0 16 534.0 17 534.0 18 985.0 19 1436.0 20 1436.0 21 1436.0 22 2376.5 23 3317.0 24 3317.0 25 3317.0 26 5670.5 27 8024.0 28 8024.0 29 8024.0 30 8979.5 31 9935.0 32 9935.0 33 9935.0 34 10103.5 35 10272.0 36 10272.0 37 10272.0 38 9189.0 39 8106.0 40 8106.0 41 8106.0 42 11094.0 43 14082.0 44 14082.0 45 14082.0 46 24513.5 47 34945.0 48 34945.0 49 34945.0 50 32213.5 51 29482.0 52 29482.0 53 29482.0 54 24223.5 55 18965.0 56 18965.0 57 18965.0 58 15182.0 59 11399.0 60 11399.0 61 11399.0 62 11045.5 63 10692.0 64 10692.0 65 10692.0 66 7769.0 67 4846.0 68 4846.0 69 4846.0 70 3968.0 71 3090.0 72 3090.0 73 3090.0 74 2280.0 75 1470.0 76 1470.0 77 1470.0 78 991.5 79 513.0 80 513.0 81 513.0 82 327.0 83 141.0 84 141.0 85 141.0 86 91.0 87 41.0 88 41.0 89 41.0 90 27.0 91 13.0 92 13.0 93 13.0 94 10.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.724759961797334 2 0.1678430752016095 3 0.15201948562031298 4 0.13054461404569628 5 0.10850461427174755 6 0.21248820294883894 7 0.4125435855123735 8 0.26504512548671666 9 0.24300512571276794 10 0.23170256172612758 11 0.2175743567428271 12 0.23452820272278768 13 0.23509333092211968 14 0.2294420489287995 15 0.28934563805799346 16 0.4741425592395635 17 0.4775333284355556 18 0.22548615153347534 19 0.1887528185768942 20 0.18253640838424195 21 0.20514153635752272 22 0.2808687150680132 23 0.2870851252606654 24 0.3096902532339461 25 0.34755384258919136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 176951.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.753445869195424 #Duplication Level Percentage of deduplicated Percentage of total 1 82.05864386072082 22.77410130488101 2 8.613317043372023 4.780984566348876 3 2.7855833842394624 2.319286130058604 4 1.3500305436774587 1.498719984628513 5 0.7961718590918346 1.1048256296940961 6 0.5966198330278966 0.9934953744256885 7 0.4377927102423132 0.8505179399946877 8 0.29932803909590716 0.6645907624144537 9 0.2341681938505396 0.5849076863086391 >10 2.0138464671146408 11.74223372572068 >50 0.3563429036856038 7.072014286440879 >100 0.38281409081653434 21.42740080587281 >500 0.05090612909794339 9.357392724539562 >1k 0.022398696803095093 11.938898339088222 >5k 0.0020362451639177356 2.8906307395832744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5115 2.8906307395832744 No Hit GGTATCAACGCAGAGTACTTTTTTT 3988 2.2537312589360896 No Hit TATCAACGCAGAGTACTTTTTTTTT 3074 1.7372040847466248 No Hit GTACATGGGAAGCAGTGGTATCAAC 2695 1.5230204971997898 No Hit GTACTGGTTCACTATCGGTCAGTCA 1796 1.0149702460003052 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1763 0.9963210154223485 No Hit CCCATGTACTCTGCGTTGATACCAC 1634 0.9234194777085182 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1623 0.9172030675158659 No Hit GAGTACATGGGAAGCAGTGGTATCA 1253 0.7081056337630192 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1177 0.6651558906137858 No Hit ATACAGGGTGACAGCCCCGTACACA 1092 0.6171199936705641 No Hit GGATACCACGTGTCCCGCCCTACTC 1031 0.5826471735113111 No Hit GTACATGGGGGTTAAGCGACTAAGC 873 0.4933569180168521 No Hit GTGGTATCAACGCAGAGTACATGGG 852 0.4814892258308797 No Hit GGTATCAACGCAGAGTACATGGGGG 831 0.46962153364490733 No Hit ATCATTAACTGAATCCATAGGTTAA 789 0.4458861492729626 No Hit ACGCAGAGTACATGGGAAGCAGTGG 754 0.426106662296342 No Hit GGTTAATGAGGCGAACCGGGGGAAC 749 0.4232810212996818 No Hit GGTATCAACGCAGAGTACATGGGAA 742 0.41932512390435767 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 720 0.4068923035190533 No Hit GTATTTAGCCTTGGAGGATGGTCCC 705 0.39841538052907305 No Hit CATGTACTCTGCGTTGATACCACTG 699 0.3950246113330809 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 654 0.3695938423631401 No Hit GTATCAACGCAGAGTACATGGGAAG 653 0.36902871416380806 No Hit TATCAACGCAGAGTACATGGGAAGC 652 0.36846358596447604 No Hit GTGGTATCAACGCAGAGTACTTTTT 650 0.367333329565812 No Hit GTATCAACGCAGAGTACATGGGGGT 649 0.36676820136648 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 624 0.3526399963831795 No Hit GCGTTGATACCACTGCTTCCCATGT 623 0.35207486818384753 No Hit GTTCACTATCGGTCAGTCAGGAGTA 585 0.33059999660923084 No Hit GCCTTGGAGGATGGTCCCCCCATAT 574 0.3243835864165786 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 551 0.3113856378319422 No Hit CCCATATTCAGACAGGATACCACGT 550 0.31082050963261015 No Hit AGCGTACACGGTGGATGCCCTGGCA 526 0.2972574328486417 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 523 0.29556204825064564 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 522 0.2949969200513136 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 508 0.2870851252606654 No Hit ACTCTGCGTTGATACCACTGCTTCC 497 0.2808687150680132 No Hit ACGCAGAGTACATGGGGGTTAAGCG 497 0.2808687150680132 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 496 0.2803035868686811 No Hit ATCAACGCAGAGTACTTTTTTTTTT 471 0.2661753818853807 No Hit CTATCGGTCAGTCAGGAGTATTTAG 470 0.2656102536860487 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 469 0.26504512548671666 No Hit TATTCAGACAGGATACCACGTGTCC 469 0.26504512548671666 No Hit CGGTACTGGTTCACTATCGGTCAGT 466 0.2633497408887206 No Hit CATCTAAGTACCCCGAGGAAAAGAA 453 0.2560030742974044 No Hit GGTATCAACGCAGAGTACATGGGGA 443 0.2503517923040842 No Hit GGTTCACTATCGGTCAGTCAGGAGT 419 0.23678871552011574 No Hit GTTAATGATAGTGTGTCGAAACACA 419 0.23678871552011574 No Hit TCACGGTACTGGTTCACTATCGGTC 399 0.22548615153347534 No Hit GTACTTAGATGTTTCAGTTCCCCCG 392 0.22153025413815122 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 385 0.2175743567428271 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 380 0.214748715746167 No Hit GTGCTAATCTGCGATAAGCGTCGGT 379 0.214183587546835 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 377 0.213053331148171 No Hit GTTATAACCGGCGATTTCCGAATGG 369 0.20853230555351482 No Hit GATATGAACCGTTATAACCGGCGAT 367 0.20740204915485078 No Hit GCTTCCCATGTACTCTGCGTTGATA 340 0.1921435877728863 No Hit TATCAACGCAGAGTACATGGGGGTT 335 0.18931794677622618 No Hit GGGAAGCAGTGGTATCAACGCAGAG 334 0.1887528185768942 No Hit GAGTACATGGGGGTTAAGCGACTAA 324 0.18310153658357398 No Hit GTACTCTGCGTTGATACCACTGCTT 322 0.18197128018490996 No Hit CACTATCATTAACTGAATCCATAGG 320 0.1808410237862459 No Hit GCCCTACTCATCGAGCTCACAGCAT 316 0.17858051098891783 No Hit GGTGATATGAACCGTTATAACCGGC 308 0.1740594853942617 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 308 0.1740594853942617 No Hit GGAGTATTTAGCCTTGGAGGATGGT 307 0.17349435719492967 No Hit GTCCCGCCCTACTCATCGAGCTCAC 305 0.17236410079626563 No Hit TCATTAACCTATGGATTCAGTTAAT 302 0.1706687161982696 No Hit GGGTGACAGCCCCGTACACAAAAAT 299 0.16897333160027353 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 295 0.16671281880294544 No Hit CAGTGGTATCAACGCAGAGTACATG 288 0.16275692140762132 No Hit GGGTACTTAGATGTTTCAGTTCCCC 285 0.16106153680962526 No Hit ACATGGGAAGCAGTGGTATCAACGC 283 0.15993128041096122 No Hit GCAGTGGTATCAACGCAGAGTACAT 282 0.15936615221162923 No Hit TCGGTAAGGTGATATGAACCGTTAT 275 0.15541025481630508 No Hit ATCCATAGGTTAATGAGGCGAACCG 274 0.15484512661697306 No Hit GCAGAGTACATGGGAAGCAGTGGTA 269 0.15201948562031298 No Hit GTGGTATCCTGTCTGAATATGGGGG 268 0.15145435742098093 No Hit ATACCACTGCTTCCCATGTACTCTG 264 0.14919384462365287 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 262 0.14806358822498886 No Hit CTATCATTAACTGAATCCATAGGTT 261 0.1474984600256568 No Hit CATGGGAAGCAGTGGTATCAACGCA 256 0.14467281902899673 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 256 0.14467281902899673 No Hit GGGAAACCCAGTGTGTTTCGACACA 251 0.14184717803233665 No Hit AGCTCACAGCATGTGCATTTTTGTG 248 0.14015179343434056 No Hit GTCCCCCCATATTCAGACAGGATAC 246 0.13902153703567655 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 233 0.1316748704443603 No Hit GGGTTTCCCCATTCGGAAATCGCCG 230 0.12997948584636426 No Hit CACACACACTGATTCAGGCTCTGGG 230 0.12997948584636426 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 226 0.12771897304903618 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 223 0.1260235884510401 No Hit GATCAACGCAGAGTACTTTTTTTTT 222 0.1254584602517081 No Hit GATACCACTGCTTCCCATGTACTCT 221 0.12489333205237607 No Hit ATATCAACGCAGAGTACTTTTTTTT 220 0.12432820385304406 No Hit ATGGGAAGCAGTGGTATCAACGCAG 218 0.12319794745438004 No Hit CTGCTTCCCATGTACTCTGCGTTGA 214 0.12093743465705195 No Hit CCCTGGCAGTCAGAGGCGATGAAGG 212 0.11980717825838792 No Hit CCGTTATAACCGGCGATTTCCGAAT 210 0.11867692185972387 No Hit GCGTACACGGTGGATGCCCTGGCAG 210 0.11867692185972387 No Hit NTATCAACGCAGAGTACTTTTTTTT 209 0.11811179366039186 No Hit TAAGTACCCCGAGGAAAAGAAATCA 208 0.11754666546105984 No Hit GAGTAGGGCGGGACACGTGGTATCC 207 0.11698153726172782 No Hit GTACATGGGCATCTAAGTACCCCGA 206 0.11641640906239581 No Hit GTGTTTCGACACACTATCATTAACT 205 0.11585128086306379 No Hit GGAGGATGGTCCCCCCATATTCAGA 205 0.11585128086306379 No Hit GGTATCAACGCAGAGTACATGGGCA 204 0.11528615266373178 No Hit TTTGTGTACGGGGCTGTCACCCTGT 202 0.11415589626506772 No Hit GGGGAAACCCAGTGTGTTTCGACAC 201 0.1135907680657357 No Hit ACCATGTACTCTGCGTTGATACCAC 200 0.1130256398664037 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 198 0.11189538346773967 No Hit CCTATGGATTCAGTTAATGATAGTG 198 0.11189538346773967 No Hit AAGCAGTGGTATCAACGCAGAGTAC 197 0.11133025526840763 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 194 0.10963487067041158 No Hit ATGATAGTGTGTCGAAACACACTGG 194 0.10963487067041158 No Hit ATTTAGCCTTGGAGGATGGTCCCCC 190 0.1073743578730835 No Hit GTTGATACCACTGCTTCCCATGTAC 190 0.1073743578730835 No Hit GTATCAACGCAGAGTACATGGGGAG 186 0.10511384507575544 No Hit TTCCAGACGCTTCCACTAACACACA 185 0.10454871687642342 No Hit GAATATGGGGGGACCATCCTCCAAG 183 0.10341846047775938 No Hit GCTCTGGGCTGCTCCCCGTTCGCTC 183 0.10341846047775938 No Hit AACGCAGAGTACTTTTTTTTTTTTT 178 0.1005928194810993 No Hit AAGTACCCCGAGGAAAAGAAATCAA 178 0.1005928194810993 No Hit CAACGCAGAGTACTTTTTTTTTTTT 178 0.1005928194810993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCAA 45 1.1921766E-6 19.656471 1 AAGTACA 25 0.004939112 19.65647 1 CCGCAGA 25 0.004939112 19.65647 1 ATACATG 35 0.0017208279 16.848404 1 ACCATGT 60 1.84455E-5 14.742353 1 GGGTATC 50 0.0011531818 13.759529 1 AGGGTGA 215 0.0 12.356194 5 ATCGCCG 55 0.003105741 12.061672 19 AATCGCC 55 0.003105741 12.061672 18 GGAGCGA 55 0.003132246 12.048004 9 GGGTGAC 230 0.0 11.550354 6 ACAGGGT 235 0.0 11.304602 3 CCCCCGG 85 5.3922085E-5 11.149445 18 GGAAATC 60 0.005909335 11.065948 15 GGTGACA 245 0.0 10.843189 7 GTGACAG 245 0.0 10.818616 8 GATCAAC 65 0.008223445 10.584254 1 AGCCCCG 255 0.0 10.4150095 13 CCGTACA 255 0.0 10.412054 17 GACAGCC 255 0.0 10.4091 10 >>END_MODULE