FastQCFastQC Report
Thu 26 May 2016
SRR1512717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176951
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62233.516792784443151No Hit
GGTATCAACGCAGAGTACTTTTTTT52922.9906584308650417No Hit
TATCAACGCAGAGTACTTTTTTTTT45892.5933733067346325No Hit
GTACATGGGAAGCAGTGGTATCAAC28851.6303948550728733No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24741.3981271651474136No Hit
GTACTGGTTCACTATCGGTCAGTCA19621.1087815270894201No Hit
CCCATGTACTCTGCGTTGATACCAC19531.103695373295432No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17931.013274861402309No Hit
GAGTACATGGGAAGCAGTGGTATCA14500.8194358890314267No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13320.7527507615102487No Hit
GGATACCACGTGTCCCGCCCTACTC12440.7030194799690309No Hit
ATACAGGGTGACAGCCCCGTACACA11650.6583743522218015No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9450.5340461483687575No Hit
GGTATCAACGCAGAGTACATGGGAA9280.5244389689801131No Hit
ATCATTAACTGAATCCATAGGTTAA9120.5153969177908008No Hit
GTGGTATCAACGCAGAGTACATGGG9070.5125712767941407No Hit
ACGCAGAGTACATGGGAAGCAGTGG9050.5114410203954767No Hit
GGTATCAACGCAGAGTACATGGGGG8890.5023989692061644No Hit
TATCAACGCAGAGTACATGGGAAGC8610.48657537962486797No Hit
GGTTAATGAGGCGAACCGGGGGAAC8470.47866358483421967No Hit
GTGGTATCAACGCAGAGTACTTTTT8470.47866358483421967No Hit
CATGTACTCTGCGTTGATACCACTG8100.457753841458935No Hit
GTACATGGGGGTTAAGCGACTAAGC7840.44306050827630244No Hit
GTATCAACGCAGAGTACATGGGAAG7770.4391046108809783No Hit
GTATTTAGCCTTGGAGGATGGTCCC7070.39954563692773704No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6970.3938943549344169No Hit
GCGTTGATACCACTGCTTCCCATGT6790.38372204734644055No Hit
CCCATATTCAGACAGGATACCACGT6500.367333329565812No Hit
AGCGTACACGGTGGATGCCCTGGCA6490.36676820136648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6350.35885640657583173No Hit
ATCAACGCAGAGTACTTTTTTTTTT6320.35716102197783567No Hit
CGGTACTGGTTCACTATCGGTCAGT6280.35490050918050764No Hit
GTTCACTATCGGTCAGTCAGGAGTA6250.3532051245825116No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6250.3532051245825116No Hit
ACTTAGATGTTTCAGTTCCCCCGGT6120.3458584579911953No Hit
GTATCAACGCAGAGTACATGGGGGT5910.3339907658052229No Hit
TATTCAGACAGGATACCACGTGTCC5850.33059999660923084No Hit
CTATCGGTCAGTCAGGAGTATTTAG5790.32720922741323866No Hit
GCCTTGGAGGATGGTCCCCCCATAT5700.3221230736192505No Hit
GCCCAGAGCCTGAATCAGTGTGTGT5690.32155794541991856No Hit
TCACGGTACTGGTTCACTATCGGTC5680.3209928172205865No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5390.3046040994399579No Hit
CATCTAAGTACCCCGAGGAAAAGAA5360.30290871484196186No Hit
ACTCTGCGTTGATACCACTGCTTCC5360.30290871484196186No Hit
ACGCAGAGTACATGGGGGTTAAGCG5280.29838768924730574No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5240.2961271764499777No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5100.2882153816593294No Hit
GTTAATGATAGTGTGTCGAAACACA5090.2876502534599974No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT4770.2695661510813728No Hit
GTTATAACCGGCGATTTCCGAATGG4570.25826358709473246No Hit
GGTATCAACGCAGAGTACATGGGGA4420.24978666410475214No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4360.24639589490876007No Hit
GGTTCACTATCGGTCAGTCAGGAGT4320.24413538211143196No Hit
TATCAACGCAGAGTACATGGGGGTT3970.22435589513481133No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3960.22379076693547933No Hit
GTGCTAATCTGCGATAAGCGTCGGT3950.22322563873614729No Hit
GTACTTAGATGTTTCAGTTCCCCCG3940.22266051053681526No Hit
GGGAAGCAGTGGTATCAACGCAGAG3930.22209538233748327No Hit
CACTATCATTAACTGAATCCATAGG3930.22209538233748327No Hit
GATATGAACCGTTATAACCGGCGAT3910.22096512593881923No Hit
GGAGTATTTAGCCTTGGAGGATGGT3850.2175743567428271No Hit
GAGTACATGGGGGTTAAGCGACTAA3770.213053331148171No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3750.21192307474950695No Hit
CATGGGAAGCAGTGGTATCAACGCA3680.20796717735418277No Hit
GCTTCCCATGTACTCTGCGTTGATA3680.20796717735418277No Hit
TCATTAACCTATGGATTCAGTTAAT3590.2028810235601946No Hit
CAGTAGCGGCGAGCGAACGGGGAGC3560.2011856389621986No Hit
GGTGATATGAACCGTTATAACCGGC3480.19666461336754243No Hit
CAGTGGTATCAACGCAGAGTACATG3410.1927087159722183No Hit
GCAGTGGTATCAACGCAGAGTACAT3330.18818769037756217No Hit
ACATGGGAAGCAGTGGTATCAACGC3270.18479692118157004No Hit
ATCCATAGGTTAATGAGGCGAACCG3210.18140615198557794No Hit
CACACACACTGATTCAGGCTCTGGG3200.1808410237862459No Hit
GTCCCGCCCTACTCATCGAGCTCAC3150.1780153827895858No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3130.17688512639092177No Hit
GTACTCTGCGTTGATACCACTGCTT3120.17631999819158975No Hit
GGGTACTTAGATGTTTCAGTTCCCC3060.17292922899559765No Hit
TAAGTACCCCGAGGAAAAGAAATCA3010.17010358799893757No Hit
GGGTGACAGCCCCGTACACAAAAAT3000.16953845979960552No Hit
GCAGAGTACATGGGAAGCAGTGGTA3000.16953845979960552No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2970.1678430752016095No Hit
ATGGGAAGCAGTGGTATCAACGCAG2870.1621917932082893No Hit
TCGGTAAGGTGATATGAACCGTTAT2780.15710563941430114No Hit
GTGGTATCCTGTCTGAATATGGGGG2780.15710563941430114No Hit
ATACCACTGCTTCCCATGTACTCTG2760.1559753830156371No Hit
GCCCTACTCATCGAGCTCACAGCAT2730.15427999841764103No Hit
TTTGTGTACGGGGCTGTCACCCTGT2730.15427999841764103No Hit
CTATCATTAACTGAATCCATAGGTT2700.152584613819645No Hit
GATACCACTGCTTCCCATGTACTCT2680.15145435742098093No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2660.1503241010223169No Hit
AGCTCACAGCATGTGCATTTTTGTG2650.1497589728229849No Hit
CAACGCAGAGTACTTTTTTTTTTTT2650.1497589728229849No Hit
GGGTTTCCCCATTCGGAAATCGCCG2620.14806358822498886No Hit
GGTATCAACGCAGAGTACATGGGCA2580.14580307542766077No Hit
GGGAAACCCAGTGTGTTTCGACACA2580.14580307542766077No Hit
GCGTACACGGTGGATGCCCTGGCAG2560.14467281902899673No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2550.1441076908296647No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2520.14241230623166864No Hit
CCGTTATAACCGGCGATTTCCGAAT2500.14128204983300463No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2430.1373261524376805No Hit
CCTATGGATTCAGTTAATGATAGTG2430.1373261524376805No Hit
TTCCAGACGCTTCCACTAACACACA2420.13676102423834846No Hit
AAGCAGTGGTATCAACGCAGAGTAC2420.13676102423834846No Hit
AACGCAGAGTACTTTTTTTTTTTTT2390.13506563964035243No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2390.13506563964035243No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2350.13280512684302434No Hit
GGAGGATGGTCCCCCCATATTCAGA2330.1316748704443603No Hit
GAATATGGGGGGACCATCCTCCAAG2290.1294143576470322No Hit
GTCCCCCCATATTCAGACAGGATAC2280.1288492294477002No Hit
CTGCTTCCCATGTACTCTGCGTTGA2270.1282841012483682No Hit
ATGATAGTGTGTCGAAACACACTGG2240.12658871665037214No Hit
GAGTAGGGCGGGACACGTGGTATCC2230.1260235884510401No Hit
TACCACTGCTTCCCATGTACTCTGC2120.11980717825838792No Hit
AGGTTAATGAGGCGAACCGGGGGAA2110.1192420500590559No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2100.11867692185972387No Hit
GTGCATTTTTGTGTACGGGGCTGTC2100.11867692185972387No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2100.11867692185972387No Hit
GTTGATACCACTGCTTCCCATGTAC2090.11811179366039186No Hit
ATTCAGACAGGATACCACGTGTCCC2040.11528615266373178No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2040.11528615266373178No Hit
CCACTGCTTCCCATGTACTCTGCGT2040.11528615266373178No Hit
TCAACGCAGAGTACTTTTTTTTTTT2010.1135907680657357No Hit
AAGTACCCCGAGGAAAAGAAATCAA2010.1135907680657357No Hit
TAATGATAGTGTGTCGAAACACACT2000.1130256398664037No Hit
GGGGAAACCCAGTGTGTTTCGACAC1980.11189538346773967No Hit
CCACGTGTCCCGCCCTACTCATCGA1940.10963487067041158No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1920.10850461427174755No Hit
GTACATGGGCATCTAAGTACCCCGA1890.10680922967375149No Hit
CATTAACTGAATCCATAGGTTAATG1880.10624410147441947No Hit
CATTAACCTATGGATTCAGTTAATG1830.10341846047775938No Hit
CGGTGGATGCCCTGGCAGTCAGAGG1830.10341846047775938No Hit
CAGGGTGACAGCCCCGTACACAAAA1820.10285333227842736No Hit
GGATGGTCCCCCCATATTCAGACAG1790.1011579476804313No Hit
ATCAACGCAGAGTACATGGGAAGCA1770.10002769128176726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGG1101.8189894E-1013.81468919
CCGAATG1101.8189894E-1013.81468918
TAACTGA1800.013.1911086
ACTGAAT1950.012.6634658
GATAAGC905.3312215E-712.66346413
TTAACTG1900.012.5039055
AATCCAT2000.012.34687712
GAATCCA2000.012.34687711
AGCGTCG1001.4192119E-712.34687717
CGTCGGT1103.742025E-812.087852519
TGAATCC2050.012.04573410
ATAAGCG951.0257154E-611.99696514
ATCATTA2000.011.8988951
TTCCGAA1209.795258E-911.87199816
TCCGAAT1209.795258E-911.87199817
GCGTCGG1052.6763155E-711.75893118
ATTAACT2050.011.5889864
ACAGGGT2200.011.2307813
ATCCATA2250.010.97500213
AAATCGC700.001483246310.85439917
Produced by FastQC (version 0.11.5)