FastQCFastQC Report
Thu 26 May 2016
SRR1512716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276503
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75200.3303312141473128No Hit
GTCCTACAGTGGACATTTCTAAATT70010.30753308912836924No Hit
GTCCTAAAGTGTGTATTTCTCATTT66930.29400356599573996No Hit
CTGTAGGACGTGGAATATGGCAAGA61470.27001941135153346No Hit
CTTTAGGACGTGAAATATGGCGAGG57870.25420568301469404No Hit
GGTATCAACGCAGAGTACTTTTTTT50640.22244644527154148No Hit
TATCAACGCAGAGTACTTTTTTTTT44940.1974080420715457No Hit
GTCCTACAGTGTGCATTTCTCATTT42040.18466920535575837No Hit
CTGAAGGACCTGGAATATGGCGAGA29880.13125394519576736No Hit
CTGTAGGACCTGGAATATGGCGAGA27360.12018433535997976No Hit
ATTTAGAAATGTCCACTGTAGGACG25850.11355135486313876No Hit
GTCCTTCAGTGTGCATTTCTCATTT25240.11087180645050765No Hit
TTTCTAAATTTTCCACCTTTTTCAG24690.10845582017682384No Hit
GAATATGGCAAGAAAACTGAAAATC23820.10463416916208763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12600.013.0270554
GGTATCA19500.011.5313411
GGCGAGG17550.011.23376119
AAATGTC11250.011.13248357
GGACCTA1451.2441888E-911.1237586
TAGAAAT11450.011.1037574
TACACCG600.005933019311.069235
GTTCTAA3200.011.0466721
AGGACCT25300.011.0254795
TTTAGAA12050.010.8660642
CACCTTT10150.010.84548514
CCACCTT10250.010.82962513
ACCTTTT9950.010.7773615
GTCGAGG1855.456968E-1210.75936219
TTGGCGA803.8074024E-410.67673217
TGGCGAA1701.4551915E-1010.59056318
TGTCGAG2250.010.52862918
GTCCTAA21600.010.5011581
TGTAGGA43700.010.4651592
ATAGGAC4000.010.4369343
Produced by FastQC (version 0.11.5)