FastQCFastQC Report
Thu 26 May 2016
SRR1512716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276503
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98100.4309240971788748No Hit
CTGTAGGACGTGGAATATGGCAAGA73120.3211943933304722No Hit
GTCCTAAAGTGTGTATTTCTCATTT72640.3190858962188936No Hit
GTCCTACAGTGGACATTTCTAAATT70830.311135105027316No Hit
GGTATCAACGCAGAGTACTTTTTTT68490.3008561816083704No Hit
CTTTAGGACGTGAAATATGGCGAGG67590.2969027495241605No Hit
TATCAACGCAGAGTACTTTTTTTTT66730.2931250255325822No Hit
GTCCTACAGTGTGCATTTCTCATTT43320.19029186431996795No Hit
CTGAAGGACCTGGAATATGGCGAGA36850.16187108033681485No Hit
CTGTAGGACCTGGAATATGGCGAGA32650.14342173061050215No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31480.13828226890102935No Hit
TTTCTAAATTTTCCACCTTTTTCAG30560.1342409827705037No Hit
ATTTAGAAATGTCCACTGTAGGACG30470.1338456395620827No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26790.11768049503998018No Hit
GAATATGGCAAGAAAACTGAAAATC26540.116582319461033No Hit
GTCCTTCAGTGTGCATTTCTCATTT25950.11399062509471765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCAA350.00216017216.2974785
TCGCGAG350.002178963716.27635818
TAGGACC12400.012.879984
TCCAACG2450.012.01350118
CCAACGA1302.6411726E-911.68559119
AATGTCC10450.011.6345348
AAATGTC10550.011.434227
ACCTTTT11400.011.41010815
GGCGAGG18150.011.14235619
CACCTTT11950.011.12453514
TGGACCG600.005845579311.0913385
TACACCG951.3500054E-511.0079455
TTCCACC12050.010.95678211
CCACCTT12150.010.94213613
ATTTAGA11350.010.9023431
TAGAAAT11550.010.8647464
CCGTGCA700.001493821410.8554369
GGTATCA22950.010.7835811
CGAACCG803.7822154E-410.68441112
TGTAGGA43700.010.639052
Produced by FastQC (version 0.11.5)