FastQCFastQC Report
Thu 26 May 2016
SRR1512715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62320.3002146592621425No Hit
GGTATCAACGCAGAGTACTTTTTTT44270.21326224270755856No Hit
GTCCTACAGTGGACATTTCTAAATT41710.2009299332128364No Hit
GTCCTAAAGTGTGTATTTCTCATTT40500.19510099005322162No Hit
CTGTAGGACGTGGAATATGGCAAGA38080.18344310373399209No Hit
TATCAACGCAGAGTACTTTTTTTTT38070.18339493065002835No Hit
CTTTAGGACGTGAAATATGGCGAGG33370.16075358118706187No Hit
GTCCTACAGTGTGCATTTCTCATTT23710.11421838207807122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG957.611561E-812.97081519
TCGAACT1103.9373845E-812.06376619
TAGGACC8750.011.9252834
TTATACC1950.011.6751034
TGTCGAG1105.136353E-711.19881418
GGTATCA15000.011.0680861
GCGCCAC1303.326022E-810.94832713
GTATAGT2450.010.8261681
CTCGAAC1252.3206303E-710.6130318
GGCGAGG10300.010.49095719
CTAAGCT1106.1543396E-610.3483864
GTGTAGG2850.010.3407841
GTACTAA1251.6738431E-610.2166951
TGTAGGA25450.010.1385482
AGGACCT17500.010.1364915
AATGTCC7100.010.1236378
GTCTTAC1853.5288394E-1010.08925151
TTAGACA1606.324626E-910.0788974
GTCTAGG2155.456968E-1210.0522041
TTTAGAA8700.010.0314372
Produced by FastQC (version 0.11.5)