FastQCFastQC Report
Thu 26 May 2016
SRR1512715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80660.38856409525167546No Hit
GGTATCAACGCAGAGTACTTTTTTT56510.27222609747919885No Hit
TATCAACGCAGAGTACTTTTTTTTT55570.26769782758660554No Hit
CTGTAGGACGTGGAATATGGCAAGA45730.2202955129662673No Hit
GTCCTACAGTGGACATTTCTAAATT43470.20940839599045785No Hit
GTCCTAAAGTGTGTATTTCTCATTT42160.20309772199120554No Hit
CTTTAGGACGTGAAATATGGCGAGG40130.19331858594656257No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26850.12934473044269137No Hit
GTCCTACAGTGTGCATTTCTCATTT24530.11816857496309942No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22500.10838943891845645No Hit
CTGAAGGACCTGGAATATGGCGAGA22300.10742597723918129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG1101.8735591E-1013.81628912
TAGGACC9400.012.5389614
ACCGGGG1251.4224497E-912.15364715
TCCAACG1103.8440703E-812.08459418
GCGAACC1501.4551915E-1112.03197511
AACCGGG1451.2278178E-911.13291814
GGTCTAG1201.2442433E-711.1034881
GAACCGG1502.1445885E-910.76337613
GGTATCA20100.010.6536661
TGTAGGA27600.010.6084342
CGGGGGA1355.7332727E-810.55004217
AGGACCT16950.010.4869715
CCGGTTT1002.4030667E-510.44856712
CGGTTTC1002.4075438E-510.44680713
GGCGAAC1553.6707206E-910.41817910
TGGCGAG24300.010.35467118
TCAATAC1752.3283064E-1010.3200853
GGCGAGG11150.010.30403219
AGGACGT25950.010.2564935
GTCCTAA12900.010.2550491
Produced by FastQC (version 0.11.5)