FastQCFastQC Report
Thu 26 May 2016
SRR1512714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827557
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87050.4763189328704932No Hit
GTCCTAAAGTGTGTATTTCTCATTT77520.4241728164976523No Hit
CTGTAGGACGTGGAATATGGCAAGA76640.4193576452061414No Hit
CTTTAGGACGTGAAATATGGCGAGG59240.324148576487628No Hit
GTATCAACGCAGAGTACTTTTTTTT55170.3018784092643896No Hit
GTCCTACAGTGTGCATTTCTCATTT44040.24097743599789226No Hit
GGTATCAACGCAGAGTACTTTTTTT34710.18992567673675842No Hit
TATCAACGCAGAGTACTTTTTTTTT32770.17931041275320003No Hit
CTGAAGGACCTGGAATATGGCGAGA31610.17296314150529915No Hit
GTCCTTCAGTGTGCATTTCTCATTT29890.16355167034461854No Hit
CTGTAGGACCTGGAATATGGCGAGA28610.1565477848296934No Hit
ATTTAGAAATGTCCACTGTAGGACG27450.15020051358179254No Hit
GAATATGGCAAGAAAACTGAAAATC25960.142047553099575No Hit
TTTCTAAATTTTCCACCTTTTTCAG23980.1312134176936752No Hit
GGAATATGGCGAGAAAACTGAAAAT20490.1121168860943872No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20100.10998288972655847No Hit
GCCATATTCCACGTCCTACAGTGGA18310.10018839357678036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10950.012.9954694
GTCTTAT1453.6379788E-1212.8502171
AGCGGAC604.2324423E-412.6163188
ACCGTGC701.13242255E-412.1657338
GTGTAGG3250.012.06983951
GGTATCA11200.011.7330691
GGCGAGG19050.011.29751919
GTATTGG1405.0804374E-911.2077081
TGGCGAA855.4458716E-511.15161718
TATGTCG1451.2369128E-911.1275316
GTACCGT600.00593793411.0678096
CCACCTT9500.010.98610113
TAGAAAT10450.010.893814
ACCTTTT10050.010.85989915
CCTACAC2900.010.7957953
ATGTCGA1502.1682354E-910.75572517
GTATTAA2100.010.740721
TGGACTG2400.010.672535
AAGTCGT2850.010.6517267
AATGTCC10150.010.6275388
Produced by FastQC (version 0.11.5)