FastQCFastQC Report
Thu 26 May 2016
SRR1512714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827557
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA89540.489943678911246No Hit
GTATCAACGCAGAGTACTTTTTTTT72600.3972516315496589No Hit
GTCCTACAGTGGACATTTCTAAATT72590.3971969136940735No Hit
CTTTAGGACGTGAAATATGGCGAGG66880.3659530181548373No Hit
GTCCTAAAGTGTGTATTTCTCATTT64750.3542981149151572No Hit
TATCAACGCAGAGTACTTTTTTTTT55790.30527091631068143No Hit
GGTATCAACGCAGAGTACTTTTTTT53950.29520283088297655No Hit
CTGAAGGACCTGGAATATGGCGAGA41160.22521869358931076No Hit
GTCCTACAGTGTGCATTTCTCATTT35450.1939747980500745No Hit
CTGTAGGACCTGGAATATGGCGAGA33190.18160856268778486No Hit
ATTTAGAAATGTCCACTGTAGGACG31970.17493298430637183No Hit
GAATATGGCAAGAAAACTGAAAATC31430.1719782201047628No Hit
TTTCTAAATTTTCCACCTTTTTCAG31270.17110273441539717No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27530.15063825642647535No Hit
GGAATATGGCGAGAAAACTGAAAAT25550.13980412102057557No Hit
ATTCCAGGTCCTTCAGTGTGCATTT23510.12864167848116365No Hit
ACAGTGGACATTTCTAAATTTTCCA22850.1250303000125304No Hit
GTCCTTCAGTGTGCATTTCTCATTT22090.1208717429880436No Hit
ACCTGGAATATGGCGAGAAAACTGA20450.1118980146720458No Hit
GCCATATTCCACGTCCTACAGTGGA20260.1108583754159241No Hit
TTCCAGGTCCTTCAGTGTGCATTTC20210.11058478613799733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG500.001500114113.29954059
TAGGACC13350.012.9565364
TGTACCG604.0787316E-412.6717795
TAGAAAT10600.012.1039384
GTGCGTT550.00306959512.09049111
ACCTTTT9550.011.83275315
AATGTCC10900.011.4170368
GGTATCA16650.011.369521
TTTAGAA11800.011.3568962
GAAATGT11150.011.33206756
CACCTTT10000.011.30027914
AAATGTC10950.011.2781487
CCACCTT9650.011.21995413
TTCCACC9950.011.17046711
ACCGTTT600.0058778411.082958
AGTCGTC3100.011.0318778
CGGTTTC951.36427425E-510.99721113
ATTTAGA11300.010.9438021
TGTAGGA47350.010.9395422
TAGCACC1053.449979E-610.8615244
Produced by FastQC (version 0.11.5)