##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512714_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827557 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.525754326677635 31.0 31.0 34.0 27.0 34.0 2 30.71119751668484 31.0 31.0 34.0 27.0 34.0 3 30.78292168178612 31.0 31.0 34.0 27.0 34.0 4 34.348171356625265 37.0 35.0 37.0 30.0 37.0 5 34.04119050732754 37.0 35.0 37.0 30.0 37.0 6 33.962533589923595 37.0 35.0 37.0 30.0 37.0 7 33.91544340340684 37.0 35.0 37.0 30.0 37.0 8 33.87265185162487 37.0 35.0 37.0 30.0 37.0 9 35.3314977316713 39.0 35.0 39.0 30.0 39.0 10 35.03702976158883 38.0 35.0 39.0 28.0 39.0 11 35.18568285421467 39.0 35.0 39.0 29.0 39.0 12 35.07812068241921 38.0 35.0 39.0 29.0 39.0 13 35.090953113911084 39.0 35.0 39.0 29.0 39.0 14 36.105853333165534 40.0 36.0 41.0 28.0 41.0 15 36.091211929368 40.0 36.0 41.0 28.0 41.0 16 36.026944166447336 40.0 36.0 41.0 27.0 41.0 17 35.9897546287202 40.0 36.0 41.0 27.0 41.0 18 35.90370806491945 40.0 36.0 41.0 27.0 41.0 19 35.941185965745525 40.0 36.0 41.0 27.0 41.0 20 35.894736525317676 40.0 36.0 41.0 27.0 41.0 21 35.818681441946815 40.0 36.0 41.0 27.0 41.0 22 35.69536490517122 39.0 36.0 41.0 27.0 41.0 23 35.59479512814101 39.0 36.0 41.0 27.0 41.0 24 35.54655094204996 39.0 36.0 41.0 27.0 41.0 25 35.441924383206654 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7317375159244399 1101 2 0.7219614674292814 1101 3 0.7296753554377169 1101 4 0.6532434254321089 1101 5 0.7205623693379906 1101 6 0.9274337060688111 1101 7 0.7895395332736541 1101 8 0.9525284259811642 1101 9 1.0857459575199115 1101 10 1.0867434806544622 1101 11 1.0219802735868768 1101 12 1.175904894293268 1101 13 1.0552111951648016 1101 14 1.2633683566973843 1101 15 1.2046988519814192 1101 16 1.1326228890035779 1101 17 0.9747803251576457 1101 18 1.3045043023244531 1101 19 1.220278097211036 1101 20 1.3391546799916654 1101 21 1.343124202771719 1101 22 1.2762799388472814 1101 23 1.392127970559919 1101 24 1.3138635586610903 1101 25 1.3436678072893145 1102 1 0.6321343806687736 1102 2 0.6404253652059069 1102 3 0.6530940836164021 1102 4 0.5678184840320668 1102 5 0.605824147170587 1102 6 0.7152807611840757 1102 7 0.7846534938354708 1102 8 0.6799533679186354 1102 9 0.673077832807266 1102 10 1.0123199878953884 1102 11 0.7856795506637155 1102 12 0.900111977666576 1102 13 0.9542179987523625 1102 14 0.9470366026003632 1102 15 1.0279866892284488 1102 16 1.1452772017805017 1102 17 1.1495886442423782 1102 18 1.1981026850918894 1102 19 1.1365554634522468 1102 20 1.0632803428100672 1102 21 1.036708289916973 1102 22 0.9007097598376106 1102 23 1.2029522824688996 1102 24 1.2356138372510586 1102 25 1.0768496930162357 1103 1 0.4929091691061238 1103 2 0.4662300957990091 1103 3 0.5346850017416784 1103 4 0.6365158121262411 1103 5 0.5566671601717275 1103 6 0.6588595544425715 1103 7 0.6438974859662352 1103 8 0.6472951300069383 1103 9 0.8018937294881425 1103 10 0.7370651614368882 1103 11 0.6911881923001246 1103 12 0.805040851765014 1103 13 0.8947101986414054 1103 14 0.8649758702632084 1103 15 0.8735992690089844 1103 16 0.7367565940321157 1103 17 0.7519176146937383 1103 18 0.8390785746125431 1103 19 0.8846688438820038 1103 20 0.8416706391165363 1103 21 0.8180941768400487 1103 22 0.9629048912403348 1103 23 0.9899928404457796 1103 24 0.8890331031838556 1103 25 0.9547552496593212 1104 1 0.3614974712927044 1104 2 0.45009968952752644 1104 3 0.3529244754728076 1104 4 0.29988694222543444 1104 5 0.38080967514416386 1104 6 0.533331334156145 1104 7 0.4575268570943507 1104 8 0.39412308422685527 1104 9 0.5675890288476424 1104 10 0.7596666995905821 1104 11 0.31654653020657975 1104 12 0.42293947111159724 1104 13 0.30857933670873194 1104 14 0.46110626662900955 1104 15 0.4776881348286537 1104 16 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2315 7 -1.058179385730952 2315 8 -1.295579967971129 2315 9 -1.3214311615771024 2315 10 -1.0623313252591657 2315 11 -1.1009192000925623 2315 12 -1.251973166473654 2315 13 -1.136530056227997 2315 14 -1.5061982441414656 2315 15 -1.455623111710615 2315 16 -1.511598059188728 2315 17 -1.382687923490323 2315 18 -1.2490577034007586 2315 19 -1.3074703650451696 2315 20 -1.3824686814845677 2315 21 -1.25506436221211 2315 22 -1.1507038412395048 2315 23 -1.0736173142963636 2315 24 -1.196316067479195 2315 25 -1.3058129493984012 2316 1 -1.6098182732856579 2316 2 -1.5623402233649841 2316 3 -1.6427043442608493 2316 4 -1.6544531282232455 2316 5 -1.648734616204301 2316 6 -4.461136637784811 2316 7 -2.5488880374835077 2316 8 -1.9380291632065827 2316 9 -2.112729244657835 2316 10 -1.824315005829547 2316 11 -1.8349733349173079 2316 12 -1.75075241123308 2316 13 -1.8970144092143784 2316 14 -2.294591596828994 2316 15 -2.110234231123158 2316 16 -2.386801118147872 2316 17 -2.2752598613291966 2316 18 -2.4590534874591583 2316 19 -2.576561642849036 2316 20 -2.600227134714295 2316 21 -2.802803229991646 2316 22 -2.7871834766405996 2316 23 -2.7384480896026844 2316 24 -2.7365433547609257 2316 25 -2.731574220371378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36883.0 3 0.0 4 734.0 5 5153.0 6 5985.0 7 4758.0 8 4033.0 9 3530.0 10 3280.0 11 2988.0 12 2830.0 13 2711.0 14 2747.0 15 2640.0 16 2943.0 17 2926.0 18 3231.0 19 3582.0 20 4566.0 21 5962.0 22 7493.0 23 9274.0 24 11124.0 25 13012.0 26 14500.0 27 16983.0 28 19663.0 29 23393.0 30 29643.0 31 38136.0 32 49742.0 33 64136.0 34 86742.0 35 121243.0 36 192439.0 37 364496.0 38 664056.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.73537979097513 22.051318855244496 14.927687167218256 23.285614186562125 2 15.775354685127166 22.45396960037213 40.99380241610004 20.776873298400666 3 20.33972127818722 26.054071090532332 30.918433734214585 22.687773897065863 4 14.122245380490838 19.0566893622563 38.0869494796503 28.734115777602554 5 12.276160080071794 39.629459893197456 36.16902682437919 11.92535320235156 6 30.858614082052533 38.82681854728581 17.72722023328329 12.587347137378362 7 27.06324613816889 32.37387275467618 22.94649531917502 17.61638578797992 8 23.754884469303093 40.14026772911855 20.538577730103654 15.5662700714747 9 26.033590423188546 14.760292396156622 21.258373936309653 37.94774324434518 10 15.261949273871183 29.922330267115584 34.99543569918083 19.8202847598324 11 33.173996542169064 21.75483551769224 27.087710579879015 17.983457360259685 12 24.059494861762616 24.84316020891175 32.92308433254907 18.174260596776566 13 29.63381912646984 22.852361462490716 26.18056707059132 21.33325234044812 14 19.453134533523254 24.61359732477196 28.10603726648255 27.827230875222238 15 21.50884423573393 35.286711266318584 23.04759788959671 20.15684660835078 16 19.911005175313896 28.98175781194426 30.869778859023313 20.23745815371853 17 20.088348416475288 31.061532543527164 29.1695872641703 19.680531775827248 18 19.941691887369476 27.428670234682496 33.666423533802124 18.963214344145907 19 23.48542800017072 26.83281296339463 28.823952994890416 20.857806041544237 20 23.554025157095047 28.328270104992683 29.45731895493216 18.66038578298011 21 21.269541798149113 27.227276632137876 28.499795081630836 23.003386488082178 22 22.74769512442689 30.135116131584606 28.30867084095059 18.80851790303791 23 20.116581863110152 30.054219923099524 29.914142212801025 19.9150560009893 24 22.97214259254294 27.437064890452117 30.458092415175013 19.13270010182993 25 21.22155423880076 29.164890616270796 29.937889762125064 19.67566538280338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 183.0 1 183.0 2 437.5 3 692.0 4 692.0 5 692.0 6 1635.5 7 2579.0 8 2579.0 9 2579.0 10 3718.0 11 4857.0 12 4857.0 13 4857.0 14 7050.0 15 9243.0 16 9243.0 17 9243.0 18 16862.0 19 24481.0 20 24481.0 21 24481.0 22 41007.5 23 57534.0 24 57534.0 25 57534.0 26 86940.5 27 116347.0 28 116347.0 29 116347.0 30 150519.5 31 184692.0 32 184692.0 33 184692.0 34 201389.5 35 218087.0 36 218087.0 37 218087.0 38 221937.0 39 225787.0 40 225787.0 41 225787.0 42 229962.5 43 234138.0 44 234138.0 45 234138.0 46 234319.5 47 234501.0 48 234501.0 49 234501.0 50 213953.0 51 193405.0 52 193405.0 53 193405.0 54 162899.0 55 132393.0 56 132393.0 57 132393.0 58 110086.5 59 87780.0 60 87780.0 61 87780.0 62 70773.5 63 53767.0 64 53767.0 65 53767.0 66 40685.0 67 27603.0 68 27603.0 69 27603.0 70 20027.5 71 12452.0 72 12452.0 73 12452.0 74 8545.0 75 4638.0 76 4638.0 77 4638.0 78 3162.5 79 1687.0 80 1687.0 81 1687.0 82 1100.0 83 513.0 84 513.0 85 513.0 86 331.5 87 150.0 88 150.0 89 150.0 90 94.5 91 39.0 92 39.0 93 39.0 94 23.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12169251082182389 2 0.012694542495801773 3 0.0 4 0.0019698428010726885 5 0.06544255528008154 6 0.027796670637359053 7 0.01723612450938603 8 0.018439917332263783 9 0.016962535231459262 10 0.018221045909922372 11 0.021887142234140986 12 0.02046447798892182 13 0.012913413918143183 14 0.006785014092583706 15 0.002133996367828746 16 0.0019151249454873362 17 0.0010943571117070494 18 0.0013132285340484592 19 6.018964114388772E-4 20 5.471785558535247E-5 21 0.0 22 9.849214005363443E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1827557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.53495285838495 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44976481329573 56.85396073612713 2 10.232587844061426 13.616494996478199 3 2.2010775941346203 4.393457818901831 4 0.7730011373462155 2.057263769312336 5 0.36125937042212114 1.2018187590342828 6 0.2037447392373408 0.8133687972180244 7 0.13459858635164462 0.6268857418398353 8 0.08865690447844807 0.47190263680351047 9 0.07361356963294288 0.4408087846738763 >10 0.4083489724874258 5.0765549184546535 >50 0.03518186676608043 1.6111057840491094 >100 0.02981180895359715 4.136450492183473 >500 0.004667737171083889 2.1182894695861822 >1k 0.003111824780722593 3.9875799454996224 >5k 5.73230880659425E-4 2.594057349837906 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 8954 0.489943678911246 No Hit GTATCAACGCAGAGTACTTTTTTTT 7260 0.3972516315496589 No Hit GTCCTACAGTGGACATTTCTAAATT 7259 0.3971969136940735 No Hit CTTTAGGACGTGAAATATGGCGAGG 6688 0.3659530181548373 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6475 0.3542981149151572 No Hit TATCAACGCAGAGTACTTTTTTTTT 5579 0.30527091631068143 No Hit GGTATCAACGCAGAGTACTTTTTTT 5395 0.29520283088297655 No Hit CTGAAGGACCTGGAATATGGCGAGA 4116 0.22521869358931076 No Hit GTCCTACAGTGTGCATTTCTCATTT 3545 0.1939747980500745 No Hit CTGTAGGACCTGGAATATGGCGAGA 3319 0.18160856268778486 No Hit ATTTAGAAATGTCCACTGTAGGACG 3197 0.17493298430637183 No Hit GAATATGGCAAGAAAACTGAAAATC 3143 0.1719782201047628 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3127 0.17110273441539717 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2753 0.15063825642647535 No Hit GGAATATGGCGAGAAAACTGAAAAT 2555 0.13980412102057557 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 2351 0.12864167848116365 No Hit ACAGTGGACATTTCTAAATTTTCCA 2285 0.1250303000125304 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2209 0.1208717429880436 No Hit ACCTGGAATATGGCGAGAAAACTGA 2045 0.1118980146720458 No Hit GCCATATTCCACGTCCTACAGTGGA 2026 0.1108583754159241 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 2021 0.11058478613799733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.471785558535247E-5 0.0 4 0.0 0.0 0.0 5.471785558535247E-5 0.0 5 0.0 0.0 0.0 5.471785558535247E-5 0.0 6 0.0 0.0 0.0 5.471785558535247E-5 0.0 7 0.0 0.0 0.0 5.471785558535247E-5 0.0 8 0.0 0.0 0.0 5.471785558535247E-5 0.0 9 0.0 0.0 0.0 2.1887142234140988E-4 0.0 10 0.0 0.0 0.0 2.1887142234140988E-4 0.0 11 0.0 0.0 0.0 2.1887142234140988E-4 0.0 12 0.0 0.0 0.0 2.1887142234140988E-4 0.0 13 0.0 0.0 0.0 2.1887142234140988E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGCG 50 0.0015001141 13.2995405 9 TAGGACC 1335 0.0 12.956536 4 TGTACCG 60 4.0787316E-4 12.671779 5 TAGAAAT 1060 0.0 12.103938 4 GTGCGTT 55 0.003069595 12.090491 11 ACCTTTT 955 0.0 11.832753 15 AATGTCC 1090 0.0 11.417036 8 GGTATCA 1665 0.0 11.36952 1 TTTAGAA 1180 0.0 11.356896 2 GAAATGT 1115 0.0 11.3320675 6 CACCTTT 1000 0.0 11.300279 14 AAATGTC 1095 0.0 11.278148 7 CCACCTT 965 0.0 11.219954 13 TTCCACC 995 0.0 11.170467 11 ACCGTTT 60 0.00587784 11.08295 8 AGTCGTC 310 0.0 11.031877 8 CGGTTTC 95 1.36427425E-5 10.997211 13 ATTTAGA 1130 0.0 10.943802 1 TGTAGGA 4735 0.0 10.939542 2 TAGCACC 105 3.449979E-6 10.861524 4 >>END_MODULE