FastQCFastQC Report
Thu 26 May 2016
SRR1512713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257895
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT79610.352585040491254No Hit
GTCCTACAGTGGACATTTCTAAATT77780.3444801463309853No Hit
GTATCAACGCAGAGTACTTTTTTTT75070.3324778167275272No Hit
CTGTAGGACGTGGAATATGGCAAGA67840.3004568414385966No Hit
CTTTAGGACGTGAAATATGGCGAGG63900.2830069600224988No Hit
GGTATCAACGCAGAGTACTTTTTTT51410.22768995015268645No Hit
TATCAACGCAGAGTACTTTTTTTTT46470.20581116482387357No Hit
GTCCTACAGTGTGCATTTCTCATTT42080.186368276647054No Hit
GTACATGGGAAGCAGTGGTATCAAC39280.17396734569145156No Hit
CTGAAGGACCTGGAATATGGCGAGA33230.14717247701952482No Hit
ATTTAGAAATGTCCACTGTAGGACG29420.13029835311208005No Hit
GTCCTTCAGTGTGCATTTCTCATTT29240.12950115040779134No Hit
CTGTAGGACCTGGAATATGGCGAGA27690.12263634934308283No Hit
TTTCTAAATTTTCCACCTTTTTCAG26510.11741024272607894No Hit
GAATATGGCAAGAAAACTGAAAATC26350.11670161810004452No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25550.11315849496987239No Hit
CCCATGTACTCTGCGTTGATACCAC24610.10899532529192013No Hit
GGAATATGGCGAGAAAACTGAAAAT23870.1057179363965109No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23750.10518646792698509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13600.012.558444
GTATTAG2150.011.8802321
CCACCTT9800.011.51904713
GTGTAGG4750.011.3752011
GGCGAGG20150.011.14761719
AATGTCC11150.011.1106498
CACCTTT10200.010.97945514
TGTCGAG2350.010.88626818
GCGCAGA1458.560164E-910.8403171
TAGAAAT11500.010.8087534
GAAATGT10900.010.794376
AAATGTC11150.010.7225447
ATGTAGG3850.010.7171321
GCCGGTT2300.010.71668911
ATCCTAC3400.010.6908651
CCGGTTT2400.010.66635412
GATATAC7950.010.6272611
ACTGTTC8700.010.5437598
ATGTCCA11750.010.5435319
GTCCTAC40950.010.4837711
Produced by FastQC (version 0.11.5)