FastQCFastQC Report
Thu 26 May 2016
SRR1512713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257895
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96340.42668060295097865No Hit
GTCCTAAAGTGTGTATTTCTCATTT82270.3643659248990763No Hit
GTCCTACAGTGGACATTTCTAAATT81370.3603799113776327No Hit
CTGTAGGACGTGGAATATGGCAAGA81340.3602470442602512No Hit
CTTTAGGACGTGAAATATGGCGAGG76160.3373053219923867No Hit
GGTATCAACGCAGAGTACTTTTTTT67760.30010252912557933No Hit
TATCAACGCAGAGTACTTTTTTTTT67300.2980652333257304No Hit
GTACATGGGAAGCAGTGGTATCAAC44330.1963333104506631No Hit
GTCCTACAGTGTGCATTTCTCATTT43480.19256874212485522No Hit
CTGAAGGACCTGGAATATGGCGAGA42220.18698832319483413No Hit
ATTTAGAAATGTCCACTGTAGGACG34740.15386012192772472No Hit
CTGTAGGACCTGGAATATGGCGAGA33830.14982981936715392No Hit
TTTCTAAATTTTCCACCTTTTTCAG33240.14721676605865197No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32760.1450908921805487No Hit
GTCCTTCAGTGTGCATTTCTCATTT30440.1348158351030495No Hit
CCCATGTACTCTGCGTTGATACCAC29950.1326456721858191No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29720.13162702428589462No Hit
GAATATGGCAAGAAAACTGAAAATC29300.12976688464255423No Hit
GGAATATGGCGAGAAAACTGAAAAT26140.11577154827837433No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23240.10292772693150036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG400.005285027414.2479897
TAGGACC14650.013.4969164
CCAACGA1800.012.6592719
CCACCTT11250.011.73404213
GTCGAGG1950.011.6854819
ACCTTTT11850.011.45820215
CACCTTT11600.011.37873714
AGGACCT29250.011.3754955
TCCACCT11850.011.22129912
TTCCACC11250.011.14484711
GTCGTAC600.00579646811.10390951
GGCGAGG20450.011.09620719
TGGACCG600.005846462711.0911065
GGACCTG28700.011.0882686
CCGTTCG600.005900715511.07735216
CGTTCGC600.005901688211.07710617
AAACGGG600.00590266111.0768619
TTAGACA2250.010.9849914
GCCGGTT2350.010.91311211
CTAGGAC3850.010.8642763
Produced by FastQC (version 0.11.5)