FastQCFastQC Report
Thu 26 May 2016
SRR1512712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416651
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71440.2956157095087375No Hit
GTCCTAAAGTGTGTATTTCTCATTT69440.287339793789008No Hit
GTATCAACGCAGAGTACTTTTTTTT65770.2721534884433044No Hit
CTGTAGGACGTGGAATATGGCAAGA63110.26114652053606413No Hit
CTTTAGGACGTGAAATATGGCGAGG55380.22916010627930966No Hit
GGTATCAACGCAGAGTACTTTTTTT42580.17619424567304093No Hit
TATCAACGCAGAGTACTTTTTTTTT38420.15898034097600355No Hit
GTCCTACAGTGTGCATTTCTCATTT32210.1332836226662435No Hit
CTGAAGGACCTGGAATATGGCGAGA30200.12496632736791535No Hit
GTCCTTCAGTGTGCATTTCTCATTT27190.11251107420972248No Hit
ATTTAGAAATGTCCACTGTAGGACG26510.10969726286501444No Hit
GAATATGGCAAGAAAACTGAAAATC25050.1036558443896119No Hit
GTACATGGGAAGCAGTGGTATCAAC24510.10142134714528493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG508.815502E-515.1800064
GTATACG400.00423758914.7299691
CCGACCA456.984406E-414.7154689
TGCATCG551.9947969E-413.7902510
TAGGACC11100.012.3935864
GTGTAGG4450.011.6957051
GTTTAGG3750.010.9983781
CCTACAC2850.010.9855313
AATGTCC12050.010.9118478
CTAATAC2000.010.9106293
AAATGTC12100.010.8988687
TAGACTG3150.010.8428615
GATATAC5750.010.7592821
GAAATGT12350.010.7550656
GTCCTAG3150.010.5993421
ACCTTTT9600.010.57725515
TTTAGAA13650.010.564842
GGCGAGG16200.010.47242719
AGGACCT25050.010.3775495
TAGAAAT13550.010.3627354
Produced by FastQC (version 0.11.5)