FastQCFastQC Report
Thu 26 May 2016
SRR1512712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416651
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85610.354250572383021No Hit
CTGTAGGACGTGGAATATGGCAAGA75860.3139054832493397No Hit
GTCCTACAGTGGACATTTCTAAATT73470.30401576396426294No Hit
GTCCTAAAGTGTGTATTTCTCATTT71530.2959881257161253No Hit
CTTTAGGACGTGAAATATGGCGAGG65850.2724845250720936No Hit
GGTATCAACGCAGAGTACTTTTTTT55820.23098080773765015No Hit
TATCAACGCAGAGTACTTTTTTTTT54010.223491104011295No Hit
CTGAAGGACCTGGAATATGGCGAGA37990.15720101909626172No Hit
GTCCTACAGTGTGCATTTCTCATTT36270.15008373157729438No Hit
ATTTAGAAATGTCCACTGTAGGACG29320.12132492445123437No Hit
TTTCTAAATTTTCCACCTTTTTCAG28950.11979388004308442No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28040.11602833839060749No Hit
GTCCTTCAGTGTGCATTTCTCATTT27880.11536626513302913No Hit
GAATATGGCAAGAAAACTGAAAATC26880.1112283072731644No Hit
GTACATGGGAAGCAGTGGTATCAAC26780.11081451148717793No Hit
CTGTAGGACCTGGAATATGGCGAGA26770.11077313190857928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11800.013.1299924
TCCAACG2100.013.11311918
TATTGCG657.9829234E-411.6998545
CCAACGT1302.6375346E-911.68678219
ACCTTTT9200.011.25034815
GGCGAAA1105.002421E-711.22196719
GGCGAGG18300.011.20781519
AATCCCG3750.011.14139819
TGGCGAA1201.2842611E-711.07832418
GATATAC7300.011.0781911
ACCGTTC700.001493426710.855838
ATAGGAC4500.010.7722763
AAATGTC9000.010.7653657
CCGGTTT1605.475158E-1010.6848812
ATTTAGA10500.010.4203261
CACCTTT10050.010.39374214
TGTAGGA40050.010.3260812
TGGCGAG38650.010.26961218
AGGACCT25300.010.2576045
GGTATCA24400.010.1380941
Produced by FastQC (version 0.11.5)