FastQCFastQC Report
Thu 26 May 2016
SRR1512711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860342
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74950.2620316032138814No Hit
GGTATCAACGCAGAGTACTTTTTTT45850.1602955171094925No Hit
TATCAACGCAGAGTACTTTTTTTTT43700.1527789334282404No Hit
GTCCTACAGTGGACATTTCTAAATT42740.1494226914124255No Hit
CTGTAGGACGTGGAATATGGCAAGA40130.14029790843192877No Hit
GTCCTAAAGTGTGTATTTCTCATTT37530.13120808630576344No Hit
CTTTAGGACGTGAAATATGGCGAGG32320.11299348119910137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9500.013.181814
GGTATCA19050.012.7791551
TCGGTTT854.006295E-612.27760713
AATCCCG2250.012.21833319
CCGCTTA550.003100566812.07467313
TGCACCG1354.836693E-911.2437445
CTAGTAC1701.2732926E-1111.1612643
TGTAGGA27450.010.9559312
GTCCTAC25550.010.8727891
ATAGGAC3850.010.5959543
GTAGGAC26350.010.58523
GATATAC5050.010.4965791
GCACCGT1553.750756E-910.4049976
CTGTAGG25400.010.3959461
GGCGAGG11350.010.35672119
AGGACCT18400.010.157185
AAGTCGT2151.8189894E-1210.1487877
AGGACGT25400.010.0471595
TCCTACA28650.010.0003382
GGACGTG24850.09.9641176
Produced by FastQC (version 0.11.5)