FastQCFastQC Report
Thu 26 May 2016
SRR1512711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860342
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95380.33345662861294206No Hit
TATCAACGCAGAGTACTTTTTTTTT64640.22598696239820273No Hit
GGTATCAACGCAGAGTACTTTTTTT61920.21647761002006055No Hit
CTGTAGGACGTGGAATATGGCAAGA46600.16291758118434788No Hit
GTCCTACAGTGGACATTTCTAAATT43030.15043655618803625No Hit
GTCCTAAAGTGTGTATTTCTCATTT39900.13949380878230644No Hit
CTTTAGGACGTGAAATATGGCGAGG39750.13896939596733537No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30220.1056517017895063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGT400.005260705514.2580844
GGTATCA19600.012.7696521
GCACCGT1750.011.9425286
TAGGACC10050.011.7280444
GTCCTAC24250.010.7919611
TATACCG909.462626E-510.56191355
GTATCAA40300.010.531961
GATATAC5300.010.4143341
GTAGGAC29050.010.2416083
TGTAGGA29600.010.1817192
CTGTAGG29750.010.172311
CGTGCCG1252.5779063E-69.87817310
AATCCCG2800.09.83454219
AGGACGT27050.09.8044245
GGACGTG27100.09.7801726
AGTACCG1752.5502231E-99.7773155
TCCTACA27000.09.7184912
TGGCGAG25350.09.55160618
TAGGACA8000.09.5053894
GGATCGT900.00111439749.4997386
Produced by FastQC (version 0.11.5)