FastQCFastQC Report
Thu 26 May 2016
SRR1512710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908659
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88510.30429830378879064No Hit
GTCCTACAGTGGACATTTCTAAATT65580.22546472446581053No Hit
GTCCTAAAGTGTGTATTTCTCATTT59680.20518046288684924No Hit
CTGTAGGACGTGGAATATGGCAAGA57870.19895766399567635No Hit
GGTATCAACGCAGAGTACTTTTTTT55270.19001883685918494No Hit
TATCAACGCAGAGTACTTTTTTTTT51300.17636993542384996No Hit
CTTTAGGACGTGAAATATGGCGAGG50010.17193490195997538No Hit
GTCCTACAGTGTGCATTTCTCATTT36110.12414655688411738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG500.001515510713.2819615
TAGGACC12400.012.5474774
TACGCTG752.1013091E-411.3845395
GACCGCG600.005937411.0683017
GGCGAGG17200.010.80249219
CCCGCAC803.821005E-410.672823
GGTATCA23300.010.618091
AGGACGT37950.010.5745725
TGGCGAG36800.010.557651518
TGTAGGA38650.010.5299662
TAGGACT4250.010.4916334
CTAGGAC4250.010.4914523
GACGTGG19000.010.4857597
GTGTAGG4350.010.3817441
GGACGTG37700.010.3678826
AGGACCT25000.010.359935
GTCCTAA18800.010.3397271
TTAGGAC22350.010.2722253
GTAGGAC38200.010.2071663
AATGTCC8900.010.2011858
Produced by FastQC (version 0.11.5)