FastQCFastQC Report
Thu 26 May 2016
SRR1512710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908659
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT109700.3771497449511957No Hit
TATCAACGCAGAGTACTTTTTTTTT74380.2557192163123969No Hit
GGTATCAACGCAGAGTACTTTTTTT72860.25049344044798655No Hit
CTGTAGGACGTGGAATATGGCAAGA67950.23361280920176616No Hit
GTCCTACAGTGGACATTTCTAAATT66060.22711496947562435No Hit
GTCCTAAAGTGTGTATTTCTCATTT62270.21408490991896953No Hit
CTTTAGGACGTGAAATATGGCGAGG59640.20504294246936472No Hit
GTCCTACAGTGTGCATTTCTCATTT37540.12906291180918766No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34780.1195740030027583No Hit
CTGAAGGACCTGGAATATGGCGAGA31760.10919121148267982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA456.678861E-414.8027281
TAGGACC12050.013.0154634
GGTATCA24350.010.7861481
TTAGGAC22650.010.7012543
GGCGAGG17950.010.63221419
CCACCTT9450.010.55228913
CACCTTT10000.010.53992414
AGGACGT38250.010.4378365
AGGACCT24850.010.366585
GGACGTG38000.010.2998236
TACACCG1201.5128717E-610.2980435
TGTAGGA39300.010.2806092
GACGTGA21450.010.2741757
TAGAAAT10350.010.1940164
GGACCTG24350.010.1045496
GTCCTAC34600.010.0936031
ACCTTTT10000.010.06463315
CACCGTC856.601837E-410.057967
ACCGCCC951.6463693E-49.999148
TTTAGGA25450.09.9734632
Produced by FastQC (version 0.11.5)