FastQCFastQC Report
Thu 26 May 2016
SRR1512709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068529
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68360.33047639167737075No Hit
GTCCTACAGTGGACATTTCTAAATT55260.2671463634302444No Hit
GTCCTAAAGTGTGTATTTCTCATTT50560.24442490291409982No Hit
CTGTAGGACGTGGAATATGGCAAGA47400.22914834648196858No Hit
GGTATCAACGCAGAGTACTTTTTTT44050.21295326292258895No Hit
CTTTAGGACGTGAAATATGGCGAGG41500.20062566200425522No Hit
TATCAACGCAGAGTACTTTTTTTTT41120.19878860774975843No Hit
GTCCTACAGTGTGCATTTCTCATTT32550.15735819995755437No Hit
CTGTAGGACCTGGAATATGGCGAGA21330.10311675591688588No Hit
CTGAAGGACCTGGAATATGGCGAGA21190.10243994645470283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG655.525383E-513.1356687
TAGGACC10500.012.6488534
AGGACGG1353.8016879E-1011.9461395
GGTATCA15550.011.6121281
TGTAGGA34150.010.9725682
GTGTAGG4050.010.90391
ACTATCG2350.010.89530711
TACGCTG700.001509371810.8418745
GGCGAGG15200.010.72653719
GTCCTAA16350.010.6838231
CTATCGG2400.010.66961812
GTATTAG1302.4590554E-710.5683971
AATGTCC7350.010.5537148
AGGACCT19950.010.509015
CGACTAA1553.7198333E-910.40976417
TGGCGAG32150.010.3757718
CTGTAGG31500.010.3431281
GTATTGG1905.2750693E-1110.3300111
GTCCTAG2850.010.3300111
GATCTAG951.1844437E-410.3300111
Produced by FastQC (version 0.11.5)